Kasugamycin water-aqua extracting process

Abstract

The invention relates to a kasugamycin water-aqua extracting process, which comprises the following steps: carrying out plate-frame filtering on streptomyces microaureus fermentation liquor so as to remove mycelia; carrying out continuous centrifugalization on the filtrate; feeding the obtained centrifugate into an ion-exchange column and carrying out ion-exchange, and desorbing the obtained product at room temperature by using ammonium chloride saturated solution; starting a nanofiltration device to carry out ammonium removal treatment; concentrating the filtrate, so that the kasugamycin content is 0.5-4%; and sequentially carrying out re-filtrating, color blending and packaging on the obtained product. Compared with original processes, according to the invention, continuous centrifugalization is newly increased for removing undissolved substances left out by plate-frame filtering, thereby saving a process of rinsing by using a plenty of water, shortening the production cycle, and reducing the production cost; according to the invention, a step of ammonium removal by using a nanofiltration membrane is added, so that ammonium salts in desorption liquid can be effectively and quickly removed, and then used for recycling; and kasugamycin is subjected to certain-procedure concentration, so that the concentration efficiency is increased, the product appearance is improved, and the product quality is enhanced.

Description

technical field [0001] The invention belongs to the field of microorganism-derived biological fungicides, and in particular relates to a method for extracting and preparing a kasugamycin aqueous solution. Background technique [0002] Kasugamycin is a metabolite produced by actinomycetes. It is a systemic antibiotic with both therapeutic and preventive effects. The pure product is a white needle-like crystalline solid, stable at room temperature, stable under acidic and neutral conditions, and easily decomposed under alkaline conditions. Kasugamycin is an ideal agent for preventing and treating various bacterial and fungal diseases, and has the functions of prevention, treatment and growth regulation. It is not only a special antibiotic for the control of rice blast, but also effective against rice thin strip disease, citrus gum disease, sand skin disease, kiwi fruit canker, pepper bacterial scab, early blight of celery, bean blight, rhomboid flax spot, Cucumber anthracnos...

AI Technical Summary

Problems solved by technology

However, since the kasugamycin fermentation broth contains a large amount of insoluble mycelium, protein and some macromolecular impurities, in the actual production process, although plate and frame filtration has removed most of the large particle impurities, there are still many small particle impurities that need to be decomposed. Repeat plate and frame filtration many times, the time period is long, and a small amount of impurities are still easily adsorbed on the resin, requiring several times the volume of water to clean the adsorbed resin, resulting in a large amount of waste water; at the same time, the filtrate is directly concentrated, and the steam consumption is large. The production cost is high, the cycle is long, and there are problems such as easy impurity precipitation after the finished product is mixed