Kasugamycin water-aqua extracting process
A technology of kasugamycin and extraction process, which is applied in the direction of biocide, disinfectant, bactericide, etc., can solve the problems of easy impurity precipitation, high production cost, and large steam consumption, so as to reduce production cost and improve product appearance , Improve the effect of concentration efficiency
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Embodiment 1
[0014] A kasugamycin water extraction process, comprising the following steps: (1) Plate and frame filtration of Streptomyces aureus fermentation broth to remove mycelium; (2) Continuous centrifugation of the filtrate to remove small molecule insolubles, the continuous centrifugation speed is 1200r / min; (3) enter the separation column for separation, absorb kasugamycin molecules and remove other soluble impurities; (4) use saturated ammonium chloride solution to desorb at room temperature; (5) start the sodium filter device, operating pressure 1.5MPa, The temperature is 25°C, and the ammonium removal treatment is carried out for 30 minutes; (6) Concentrate the filtrate so that the content of kasugamycin is between 0.5%; (7) Complete refiltration, color matching, and packaging.
Embodiment 2
[0016] A kasugamycin water extraction process, comprising the following steps: (1) Streptomyces aureus fermented liquid plate-and-frame filtration to remove mycelium; (2) Filtrate continuous centrifugation to remove small molecule insoluble matter, the continuous centrifugation speed is 1200r / min; (3) enter the separation column for separation, absorb kasugamycin molecules and remove other soluble impurities; (4) use ammonium chloride saturated solution to desorb at room temperature; (5) start the sodium filter device, operating pressure 2MPa, The temperature is 28°C, and the ammonium removal treatment is carried out for 35 minutes; (6) Concentrate the filtrate so that the content of kasugamycin is between 2%; (7) Complete refiltration, color matching, and packaging.
Embodiment 3
[0018] A kasugamycin water extraction process, comprising the following steps: (1) Streptomyces aureus fermented liquid plate-and-frame filtration to remove mycelium; (2) Filtrate continuous centrifugation to remove small molecule insoluble matter, the continuous centrifugation speed is 1200 r / min; (3) enter the separation column for separation, absorb kasugamycin molecules and remove other soluble impurities; (4) use saturated ammonium chloride solution to desorb at room temperature; (5) start the sodium filter device, operating pressure 2MPa, temperature 25°C, deammonium treatment for 35 minutes; (6) Concentrate the filtrate so that the content of kasugamycin is between 4%; (7) Complete multiple filtration, color matching, and packaging.
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