Kasugamycin water-aqua extracting process

A technology of kasugamycin and extraction process, which is applied in the direction of biocide, disinfectant, bactericide, etc., can solve the problems of easy impurity precipitation, high production cost, and large steam consumption, so as to reduce production cost and improve product appearance , Improve the effect of concentration efficiency

Inactive Publication Date: 2015-07-15
SHANXI XINYUAN HUAKANG CHEM
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the kasugamycin fermentation broth contains a large amount of insoluble mycelium, protein and some macromolecular impurities, in the actual production process, although plate and frame filtration has removed most of the large particle impurities, there are still many small particle impurities that need to be decomposed. Repeat plate and frame filtration many times, the time period is long, and a sm

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A kasugamycin water extraction process, comprising the following steps: (1) Plate and frame filtration of Streptomyces aureus fermentation broth to remove mycelium; (2) Continuous centrifugation of the filtrate to remove small molecule insolubles, the continuous centrifugation speed is 1200r / min; (3) enter the separation column for separation, absorb kasugamycin molecules and remove other soluble impurities; (4) use saturated ammonium chloride solution to desorb at room temperature; (5) start the sodium filter device, operating pressure 1.5MPa, The temperature is 25°C, and the ammonium removal treatment is carried out for 30 minutes; (6) Concentrate the filtrate so that the content of kasugamycin is between 0.5%; (7) Complete refiltration, color matching, and packaging.

Embodiment 2

[0016] A kasugamycin water extraction process, comprising the following steps: (1) Streptomyces aureus fermented liquid plate-and-frame filtration to remove mycelium; (2) Filtrate continuous centrifugation to remove small molecule insoluble matter, the continuous centrifugation speed is 1200r / min; (3) enter the separation column for separation, absorb kasugamycin molecules and remove other soluble impurities; (4) use ammonium chloride saturated solution to desorb at room temperature; (5) start the sodium filter device, operating pressure 2MPa, The temperature is 28°C, and the ammonium removal treatment is carried out for 35 minutes; (6) Concentrate the filtrate so that the content of kasugamycin is between 2%; (7) Complete refiltration, color matching, and packaging.

Embodiment 3

[0018] A kasugamycin water extraction process, comprising the following steps: (1) Streptomyces aureus fermented liquid plate-and-frame filtration to remove mycelium; (2) Filtrate continuous centrifugation to remove small molecule insoluble matter, the continuous centrifugation speed is 1200 r / min; (3) enter the separation column for separation, absorb kasugamycin molecules and remove other soluble impurities; (4) use saturated ammonium chloride solution to desorb at room temperature; (5) start the sodium filter device, operating pressure 2MPa, temperature 25°C, deammonium treatment for 35 minutes; (6) Concentrate the filtrate so that the content of kasugamycin is between 4%; (7) Complete multiple filtration, color matching, and packaging.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a kasugamycin water-aqua extracting process, which comprises the following steps: carrying out plate-frame filtering on streptomyces microaureus fermentation liquor so as to remove mycelia; carrying out continuous centrifugalization on the filtrate; feeding the obtained centrifugate into an ion-exchange column and carrying out ion-exchange, and desorbing the obtained product at room temperature by using ammonium chloride saturated solution; starting a nanofiltration device to carry out ammonium removal treatment; concentrating the filtrate, so that the kasugamycin content is 0.5-4%; and sequentially carrying out re-filtrating, color blending and packaging on the obtained product. Compared with original processes, according to the invention, continuous centrifugalization is newly increased for removing undissolved substances left out by plate-frame filtering, thereby saving a process of rinsing by using a plenty of water, shortening the production cycle, and reducing the production cost; according to the invention, a step of ammonium removal by using a nanofiltration membrane is added, so that ammonium salts in desorption liquid can be effectively and quickly removed, and then used for recycling; and kasugamycin is subjected to certain-procedure concentration, so that the concentration efficiency is increased, the product appearance is improved, and the product quality is enhanced.

Description

technical field [0001] The invention belongs to the field of microorganism-derived biological fungicides, and in particular relates to a method for extracting and preparing a kasugamycin aqueous solution. Background technique [0002] Kasugamycin is a metabolite produced by actinomycetes. It is a systemic antibiotic with both therapeutic and preventive effects. The pure product is a white needle-like crystalline solid, stable at room temperature, stable under acidic and neutral conditions, and easily decomposed under alkaline conditions. Kasugamycin is an ideal agent for preventing and treating various bacterial and fungal diseases, and has the functions of prevention, treatment and growth regulation. It is not only a special antibiotic for the control of rice blast, but also effective against rice thin strip disease, citrus gum disease, sand skin disease, kiwi fruit canker, pepper bacterial scab, early blight of celery, bean blight, rhomboid flax spot, Cucumber anthracnos...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01N43/16A01N25/02A01P1/00A01P3/00C07H15/207C07H1/08
Inventor 马爱民
Owner SHANXI XINYUAN HUAKANG CHEM
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap