Method for increasing fermentation level of tetracycline

Abstract

The present invention relates to a method for increasing the fermentation level of tetracycline. The method is characterized in that during a fermentation process of tetracycline production using Streptomyces aureus as a fermentation strain, the carbon source and the nitrogen source of a material supplementation culture medium and other substances are subjected to separated sterilization, and the separated material supplementation is performed according to the requirements of the strain on the carbon source and the nitrogen source. According to the present invention, the carbon source and the nitrogen source in the material supplementation culture medium during the fermentation production process using the Streptomyces aureus as the fermentation strain are respectively subjected to the sterilization treatment, the quality of the material obtained through the method is good, the adverse effect of the toxic substance produced from the sugar and nitrogen mixed sterilization on the bacteria is reduced, the utilization efficiency of the sugar water is increased, and the fermentation level is increased; and during the material supplementation process, the material supplementation amount is flexibly controlled according to the requirements of the strain on the carbon source and the nitrogen source, such that the requirements of the bacterial growth reproduction and synthesis at the growth stage can be completely met, the high degree utilization of the carbon source and the nitrogen source can be ensured, the supplementation amount during the process can be reduced, the fermentation unit can be increased, and the material consumption can be reduced.

Description

Technical field [0001] The present invention relates to biology and new medical technology, in particular to a method for improving the fermentation level of tetracycline. Background technique [0002] Tetracycline is an antibacterial substance produced by fermentation of Streptomyces aureus as a fermentation strain, which has a good inhibitory effect on Gram-positive bacteria, negative bacteria, Rickettsia, filtering viruses, spirochetes and protozoa. Is a kind of spectrum antibiotic. [0003] In the prior art, during the fermentation process of using Streptomyces aureus to make a growing strain, a mixed sugar water containing a large amount of organic carbon and nitrogen source needs to be supplemented throughout the whole process. Due to the complex structure of the nitrogen source material, a higher temperature is required for sterilization. The mixture of carbon source and nitrogen source is prone to produce substances such as 5-hydroxymethyl furfural during high temperature ...

AI Technical Summary

Problems solved by technology

[0003] In the prior art, during the fermentation process using Streptomyces aureus as the starting strain, mixed sugar water containing a large amount of organic carbon and nitrogen sources needs to be added throughout the whole process. Due to the complex structure of nitrogen source substances, higher temperatures are required for sterilization, and the mixed sugar water The mixture of carbon source and nitrogen source is easy to produce substances such as 5-hydroxy-methylfurfural during high-temperature sterilization. This substance has a certain toxic effect on the growth of bacteria, affects the vitality of bacteria, and ultimately affects the fermentation level.