Method for increasing fermentation level of tetracycline

A fermentation level and tetracycline technology, applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of affecting the vitality of the bacteria, affecting the fermentation level, and the toxicity of the growth of the bacteria, so as to reduce the amount of supplementation in the process , improve the fermentation level, improve the effect of dissolved oxygen

Inactive Publication Date: 2016-10-26
NINGXIA QIYUAN PHARMA
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, during the fermentation process using Streptomyces aureus as the starting strain, mixed sugar water containing a large amount of organic carbon and nitrogen sources needs to be added throughout the whole process. Due to the complex structure of nitrogen source substances, higher temperatures are required for ste

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Preparation of slant spores: inoculate Streptomyces aureus on a solid medium under aseptic conditions, cultivate at a constant temperature of 35°C for 4 to 5 days, and then inoculate the seed medium.

[0026] 2. Seed culture: inoculate the slant spores grown in step 1 aseptically in a sterile seed culture medium, the inoculum is 15% of the 500ml eggplant-shaped bottle, cultured at a constant temperature of 32℃, and the aeration ratio is 0.6~0.8v / v / min, stirring speed of 400-600r / min, culture for 20-26 hours.

[0027] 3. Fermentation culture: Inoculate the seeds cultivated in step 2 aseptically in a sterile fermentation medium, the inoculum amount is 10-20%, and the culture temperature is controlled 0-40hr: 33℃, 41-80hr: 32℃, 80hr ~ Put the tank at 31℃, the process ventilation ratio: 0.8~1.5v / min, ensure that the dissolved oxygen in the process is not less than 20%, and cultivate for 6~8 days.

[0028] 4. Sugar water preparation and sterilization:

[0029] 4.1 Sugar water c...

experiment example 2

[0045] 1. Preparation of slant spores: inoculate Streptomyces aureus on a solid medium under aseptic conditions, cultivate at a constant temperature of 35°C for 4 to 5 days, and then inoculate the seed medium.

[0046] 2. Seed culture: inoculate the slant spores grown in step 1 aseptically into the seed culture medium, the inoculum is 15% of the 500ml eggplant-shaped bottle, cultured at a constant temperature of 32℃, and the aeration ratio is 0.6~0.8v / v / min, the stirring speed is 400-600r / min, culture for 20-26 hours.

[0047] 3. Fermentation culture: inoculate the seeds cultivated in step 2 aseptically into the fermentation medium, the inoculum amount is 10-20%, and the culture temperature is controlled 0-40hr: 33℃, 41-80hr: 32℃, 80hr~ Tank 31℃, process ventilation ratio: 0.8-1.5v / min, ensure that the process oxygen is not less than 20%, and cultivate for 6-8 days.

[0048] 4. Sugar water preparation and sterilization:

[0049] 4.1 Sugar water composition: starch 400g / l, amylase 0....

experiment example 3

[0065] 1. Preparation of slant spores: inoculate Streptomyces aureus on a solid medium under aseptic conditions, cultivate at a constant temperature of 35°C for 4 to 5 days, and inoculate the seed medium.

[0066] 2. Seed culture: inoculate the slant spores grown in step 1 aseptically into the seed culture medium, the inoculum is 15% of the 500ml eggplant-shaped bottle, cultured at a constant temperature of 32℃, and the aeration ratio is 0.6~0.8v / v / min, the stirring speed is 400-600r / min, culture for 20-26 hours.

[0067] 3. Fermentation culture: inoculate the seeds cultivated in step 2 into the fermentation medium aseptically, the inoculum amount is 10-20%, and the culture temperature is controlled 0-40hr: 32℃, 41-80hr: 31℃, 80hr~ Tank 30℃, process ventilation ratio: 0.8~1.5v / min, ensure that the dissolved oxygen in the process is not less than 20%, culture for 6~8 days.

[0068] 4. Sugar water preparation and sterilization:

[0069] 4.1 Sugar water composition: starch 500g / l, amyl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention relates to a method for increasing the fermentation level of tetracycline. The method is characterized in that during a fermentation process of tetracycline production using Streptomyces aureus as a fermentation strain, the carbon source and the nitrogen source of a material supplementation culture medium and other substances are subjected to separated sterilization, and the separated material supplementation is performed according to the requirements of the strain on the carbon source and the nitrogen source. According to the present invention, the carbon source and the nitrogen source in the material supplementation culture medium during the fermentation production process using the Streptomyces aureus as the fermentation strain are respectively subjected to the sterilization treatment, the quality of the material obtained through the method is good, the adverse effect of the toxic substance produced from the sugar and nitrogen mixed sterilization on the bacteria is reduced, the utilization efficiency of the sugar water is increased, and the fermentation level is increased; and during the material supplementation process, the material supplementation amount is flexibly controlled according to the requirements of the strain on the carbon source and the nitrogen source, such that the requirements of the bacterial growth reproduction and synthesis at the growth stage can be completely met, the high degree utilization of the carbon source and the nitrogen source can be ensured, the supplementation amount during the process can be reduced, the fermentation unit can be increased, and the material consumption can be reduced.

Description

Technical field [0001] The present invention relates to biology and new medical technology, in particular to a method for improving the fermentation level of tetracycline. Background technique [0002] Tetracycline is an antibacterial substance produced by fermentation of Streptomyces aureus as a fermentation strain, which has a good inhibitory effect on Gram-positive bacteria, negative bacteria, Rickettsia, filtering viruses, spirochetes and protozoa. Is a kind of spectrum antibiotic. [0003] In the prior art, during the fermentation process of using Streptomyces aureus to make a growing strain, a mixed sugar water containing a large amount of organic carbon and nitrogen source needs to be supplemented throughout the whole process. Due to the complex structure of the nitrogen source material, a higher temperature is required for sterilization. The mixture of carbon source and nitrogen source is prone to produce substances such as 5-hydroxymethyl furfural during high temperature ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P29/00C12R1/49
CPCC12P29/00
Inventor 章惠霞孙瑞君张志
Owner NINGXIA QIYUAN PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap