Purification process of tetracycline

Abstract

The invention discloses a purification process of tetracycline. The purification process comprises the following steps: 1) preparing a tetracycline crude product: conducting fermentation cultivation of streptomyces aureus spores in a fermentation tank, adding oxalic acid and conducting acidifying treatment; conducting filtration so as to obtain a filtrate, adding calcium salt to the filtrate under an alkaline condition, conducting precipitation, and conducting acid pickling on a precipitate so as to obtain the tetracycline crude product; 2) drying the tetracycline crude product; 3) preparing a tetracycline-inorganic salt dissolved solution, namely, dissolving the dried tetracycline crude product by virtue of a saturated inorganic salt solution, so that the tetracycline-inorganic salt dissolved solution is obtained; and 4) precipitating the tetracycline-inorganic salt dissolved solution, namely, dropping ammonia water to the tetracycline-inorganic salt dissolved solution to regulate pH value to 3-5, decreasing temperature to 0-10 DEG C, keeping the dissolved solution for 1-2h until a tetracycline-inorganic salt precipitate is separated out, and then sequentially conducting vacuum filtration and vacuum drying treatment, so that a finished tetracycline product is obtained. The purification process provided by the invention has fewer steps, so that the loss of the tetracycline in a purifying process is reduced.

Description

technical field

[0001] The invention relates to the technical field of drug synthesis, in particular to a purification process of tetracycline. Background technique

[0002] Tetracycline is not yellow crystal, odorless. Bitter. Hygroscopic. When exposed to light, the color gradient becomes darker. Soluble in water, in methanol. Ethanol, insoluble in ether, hydroxyl. It is an antibacterial substance isolated from the culture solution of the actinomycetes Streptomycesaureofaciens, and it is effective against Gram-positive bacteria, negative bacteria, Rickettsia, filter viruses, spirochetes and even protozoa. It has a good inhibitory effect, but it is ineffective against Mycobacterium tuberculosis and Proteus.

[0003] Tetracycline is the first generation of tetracycline antibiotics, which has broad-spectrum antibacterial characteristics and can resist various microorganisms such as Gram-negative and Gram-positive bacteria, chlamydia, mycoplasma and protozoan parasites. ...