Purification process of tetracycline

A tetracycline and process technology, applied in the field of drug synthesis, can solve problems such as tetracycline loss, and achieve the effects of less loss and fewer steps

Inactive Publication Date: 2017-02-15
CHINA CHENGDU ANIMAL HUSBANDRY IND BIOPHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a purification process of tetracycline, which sol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A purification process for tetracycline, comprising the following steps:

[0023] 1) Preparation of crude tetracycline: ferment Streptomyces aureus spores in a fermenter, add oxalic acid for acidification, filter to obtain the filtrate, add calcium salt to the filtrate under alkaline conditions for precipitation, and pickle the precipitate to obtain Crude tetracycline;

[0024] The specific steps of the fermentation culture are: inoculating Streptomyces aureus spores on the spore culture medium and culturing for 5 days until the spores mature, then transferring the prepared spores to a seed tank for cultivation, and after the cultivation of the seed tank is completed, transfer to fermentation culture;

[0025] The spore medium is composed of the following components: bran 3.0%, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.2%, agar 2.4%, and the balance is water;

[0026] The seed tank is provided with a medium, and the medium is composed of the following co...

Embodiment 2

[0032] A purification process for tetracycline, comprising the following steps:

[0033] 1) Preparation of crude tetracycline: ferment Streptomyces aureus spores in a fermenter, add oxalic acid for acidification, filter to obtain the filtrate, add calcium salt to the filtrate under alkaline conditions for precipitation, and pickle the precipitate to obtain Crude tetracycline;

[0034] The specific steps of the fermentation culture are: inoculating Streptomyces aureus spores on the spore culture medium and culturing for 5 days until the spores mature, then transferring the prepared spores to a seed tank for cultivation, and after the cultivation of the seed tank is completed, transfer to fermentation culture;

[0035] The spore medium is composed of the following components: bran 3.8%, magnesium sulfate 0.3%, potassium dihydrogen phosphate 0.4%, agar 3.2%, and the balance is water;

[0036] The seed tank is provided with a medium, and the medium is composed of the following co...

Embodiment 3

[0042] A purification process for tetracycline, comprising the following steps:

[0043] 1) Preparation of crude tetracycline: ferment Streptomyces aureus spores in a fermenter, add oxalic acid for acidification, filter to obtain the filtrate, add calcium salt to the filtrate under alkaline conditions for precipitation, and pickle the precipitate to obtain Crude tetracycline;

[0044] The specific steps of the fermentation culture are: inoculating Streptomyces aureus spores on the spore medium and culturing for 5 days until the spores mature, then transferring the prepared spores to a seed tank for cultivation, and then transferring to fermentation culture after the cultivation of the seed tank is completed;

[0045] The spore medium is composed of the following components: bran 3.3%, magnesium sulfate 0.2%, potassium dihydrogen phosphate 0.3%, agar 2.8%, and the balance is water;

[0046] The seed tank is provided with a medium, and the medium is composed of the following co...

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PUM

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Abstract

The invention discloses a purification process of tetracycline. The purification process comprises the following steps: 1) preparing a tetracycline crude product: conducting fermentation cultivation of streptomyces aureus spores in a fermentation tank, adding oxalic acid and conducting acidifying treatment; conducting filtration so as to obtain a filtrate, adding calcium salt to the filtrate under an alkaline condition, conducting precipitation, and conducting acid pickling on a precipitate so as to obtain the tetracycline crude product; 2) drying the tetracycline crude product; 3) preparing a tetracycline-inorganic salt dissolved solution, namely, dissolving the dried tetracycline crude product by virtue of a saturated inorganic salt solution, so that the tetracycline-inorganic salt dissolved solution is obtained; and 4) precipitating the tetracycline-inorganic salt dissolved solution, namely, dropping ammonia water to the tetracycline-inorganic salt dissolved solution to regulate pH value to 3-5, decreasing temperature to 0-10 DEG C, keeping the dissolved solution for 1-2h until a tetracycline-inorganic salt precipitate is separated out, and then sequentially conducting vacuum filtration and vacuum drying treatment, so that a finished tetracycline product is obtained. The purification process provided by the invention has fewer steps, so that the loss of the tetracycline in a purifying process is reduced.

Description

technical field [0001] The invention relates to the technical field of drug synthesis, in particular to a purification process of tetracycline. Background technique [0002] Tetracycline is not yellow crystal, odorless. Bitter. Hygroscopic. When exposed to light, the color gradient becomes darker. Soluble in water, in methanol. Ethanol, insoluble in ether, hydroxyl. It is an antibacterial substance isolated from the culture solution of the actinomycetes Streptomycesaureofaciens, and it is effective against Gram-positive bacteria, negative bacteria, Rickettsia, filter viruses, spirochetes and even protozoa. It has a good inhibitory effect, but it is ineffective against Mycobacterium tuberculosis and Proteus. [0003] Tetracycline is the first generation of tetracycline antibiotics, which has broad-spectrum antibacterial characteristics and can resist various microorganisms such as Gram-negative and Gram-positive bacteria, chlamydia, mycoplasma and protozoan parasites. ...

Claims

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Application Information

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IPC IPC(8): C12P29/00C07C231/24C07C237/26C12R1/49
CPCC12P29/00C07C231/24C07C237/26
Inventor 廖成斌
Owner CHINA CHENGDU ANIMAL HUSBANDRY IND BIOPHARM
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