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Kasugamycin raw powder, and preparation method and application thereof

A technology for kasugamycin and raw powder, applied in the field of kasugamycin raw powder and its preparation, can solve the problems of unstable physicochemical properties and use effect of products, affecting the popularization and use of kasugamycin, crop, human or animal injury and the like , to reduce the number of miscellaneous algae, improve the control effect, and reduce the production cost.

Inactive Publication Date: 2020-05-22
枣庄市杰诺生物酶有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When using actinomycetes to prepare, the process of adding chemical reagents to the fermentation broth, such as saturated ammonium chloride, to purify kasugamycin is often adopted, but the content of kasugamycin prepared by the above method is generally low, which cannot It meets the needs of current agricultural production, and will also produce a lot of impurities, resulting in unstable physical and chemical properties and use effects of the product, and may even cause phytotoxicity; in addition, the chemical reagents added in the preparation process are relatively difficult to decompose, and are easy to cause in the product. residues, thereby causing harm to crops, humans or animals
And above-mentioned these shortcoming or defective can influence the popularization and use of kasugamycin and its products

Method used

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  • Kasugamycin raw powder, and preparation method and application thereof
  • Kasugamycin raw powder, and preparation method and application thereof
  • Kasugamycin raw powder, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation method of kasugamycin former powder of the present invention comprises the following steps:

[0035] (1) Pick Streptomyces aureus strains and inoculate them in the shake flask seed fermentation medium, and cultivate them for 24 to 36 hours at 27 to 29° C. at a rotating speed of 180 to 200 rpm to obtain a bacterial concentration of 10 7 ~10 8 CFU / mL shake flask seed liquid, including 1.5% soybean cake powder and 0.5% yeast powder in the shake flask seed fermentation medium.

[0036] (2) Inoculate the shake flask seed liquid into the fermentation medium of the primary seed tank according to the volume ratio of 8 to 10%, at 27 to 29°C, at a speed of 80 to 100rpm, and with an air volume of 18 to 20m 3 24~30h of fermenting and cultivating under / h, obtains primary seed liquid, comprises 0.5% yeast powder, 1.5% soybean cake powder or soybean meal, 1% soybean oil and 0.2L / m 3 defoamer.

[0037] (3) Inoculate the primary seed liquid into the secondary seed ta...

Embodiment 2

[0043] Before preparing kasugamycin, various culture media and seed tanks were sterilized at 121-123° C. for 35-40 minutes.

[0044] In this example, Streptomyces aureus was purchased from Biological Company.

[0045] The concrete preparation method of described kasugamycin former powder is as follows:

[0046] 1. Shake flask seed solution preparation

[0047]Pick Streptomyces microaureus from the inclined surface of the test tube and inoculate it in the seed fermentation medium of the shake flask, and cultivate it at 28°C and 180rpm for 24-36h, and the concentration of the prepared bacteria is 10 7 ~10 8 CFU / mL shake flask seed solution, wherein the formula of shake flask seed fermentation medium is: yeast powder 0.5%, soybean cake powder 1.5%, maltose 0.5%, corn steep liquor 0.2%, potassium dihydrogen phosphate 0.1%, sodium chloride 0.2 %, magnesium phosphate 0.04%, soybean oil 1%.

[0048] 2. Preparation of seed liquid in primary seed tank

[0049] Take the shake flask...

Embodiment 3

[0061] 1. During the fermentation process of Streptomyces aureus, check and record the pH, stirring speed and air flow in the seed tank and fermenter every 6 to 12 hours. The results are shown in Table 2 and Table 3. The results show that: in the fermentation process, by gradually increasing the stirring speed and air flow, the oxygen required by Streptomyces aureus can be effectively maintained, and the stability of the pH of the fermented liquid can be well controlled by supplementing ammonia, effectively ensuring the production of kasugamycin. Quality and potency.

[0062] Table 2 The determination of each index in the seed tank fermentation process

[0063]

[0064] Table 3 The determination of each index in the fermenter fermentation process

[0065]

[0066]

[0067] 2. During the fermentation process of Streptomyces aureus strains, test the bacterial concentration, reducing sugar / total sugar content in the seed tank and fermenter every 6 hours; measure the con...

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Abstract

The invention provides kasugamycin raw powder, and a preparation method and application thereof. The preparation method of the kasugamycin raw powder comprises the following steps: carrying out shake-flask culture, primary seeding tank fermentation culture, secondary seeding tank fermentation culture and fermentation tank fermentation culture on streptomyces microaureus strains; and adjusting thepH value of the obtained fermentation product with an acid solution, performing tank sterilization and filtering, performing evaporating to obtain an aqueous kasugamycin solution, adding sodium benzoate, carrying out concentrating, and then carrying out spray drying and crushing to obtain the kasugamycin raw powder. The kasugamycin raw powder prepared by the invention is prepared by fermenting streptomyces microaureofaciens step by step and strictly controlling a production process; the kasugamycin raw powder is high in content, high in quality, safe to use and free of toxic and side effects;and the preparation method is simple and efficient. The kasugamycin raw powder prepared by the invention can be used for preventing and treating plant pathogenic bacteria and preparing a purifying agent for purifying an aquaculture water body, and has wide application prospects.

Description

technical field [0001] The invention belongs to the field of pesticides, and in particular relates to a kasugamycin raw powder and a preparation method and application thereof. Background technique [0002] Kasugamycin is a metabolite produced by actinomycetes, and its pure product is white needle-like or flaky crystal, stable at room temperature, stable under acidic and neutral conditions, easily destroyed and invalidated in alkaline solution, and insoluble in Organic solvents. Kasugamycin has the functions of prevention, treatment and growth regulation, so it can be used to prevent and treat a variety of bacterial and fungal diseases, and the toxicity of kasugamycin to humans, livestock and poultry is very low, and will not cause poisoning to humans or animals symptom. Kasugamycin products generally exist in the form of kasugamycin-water hydrochloride hydrate, which not only has excellent control and therapeutic effects on rice blast, but also has excellent effects on wa...

Claims

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Application Information

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IPC IPC(8): A01N63/28A01N37/10A01P1/00A01P3/00A01P13/00C12P19/46C02F1/50C02F103/20C12R1/465
CPCA01N37/10C02F1/50C02F2103/20C12P19/46
Inventor 何增国许向阳宋在伟刘红梅
Owner 枣庄市杰诺生物酶有限公司
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