32 results about "Edta treatment" patented technology

The present invention provides a process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonic stem cells, which comprises: (a) treating embryoid bodies differentiated from embryonic stem cells with 0.005-0.015% trypsin and 0.05-0.15 mM ethylenediaminetetraacetate (EDTA) to obtain vascular endothelial cell clusters; and (b) treating the vascular endothelial cell clusters with 0.1-0.5% trypsin and 0.5-2 mM EDTA so as to separate the vascular endothelial cell clusters into single cells.

The invention provides a kit for detecting cadmium ions based on a direct competition enzyme-linked immunoadsordent assay. The kit comprises a detection plate coated with a goat anti-mouse IgG secondary antibody, a Cd-ITCBE chelate hapten with the mass concentration of 20mu g / mL to 50mu g / mL, an anti-cadmium ion monoclonal antibody solution with the mass concentration of 10mu g / mL to 20mu g / mL, 1.0mol / L to 3.0mol / L of a stopping solution, a sample diluting solution, a substrate color developing solution, a washing solution, a cadmium ion standard product solution and an EDTA (Ethylene Diamine Tetraacetic Acid) treatment solution with the mass concentration of 10mg / mL to 20mg / mL, wherein the detection plate is sealed and packaged in vacuum; the coating concentration of the goat anti-mouse IgG secondary antibody is 50mu g / mL to 200mu g / mL; the anti-cadmium ion monoclonal antibody solution is prepared from Cd-ITCBE-cBSA immunogen immunized Balb / C mice. The kit provided by the invention has the characteristics of high sensitivity and high specificity and also has the advantage of short detection time.