Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for acquiring monkey haploid neural stem cells

A neural stem cell, haploid technology, applied in the field of bioengineering

Active Publication Date: 2018-05-18
NANKAI UNIV
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] To sum up, the problem existing in the prior art is that there is a technical gap in obtaining monkey haploid neural stem cell data through in vitro differentiation.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for acquiring monkey haploid neural stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Preparation of a pluripotent monkey embryonic stem cell culture medium

[0027] After improvement, the culture medium for the growth of haploid monkey stem cells contains D / F12 medium with 20% KOSR and 0.1mM non-essential amino acids, and other supplements in the medium are 0.1mM β-mercaptoethanol, 100U / ml penicillin, 0.1 mg / ml streptomycin, 2mM L-Glutamine, 5ng / ml fibroblast growth factor (bFGF), 10ng / ml human leukemia inhibitory factor (LIF), 0.5μM PD0325901, 3μM CHIR99021, 10μM SB203580 and 10 μM of SP600125.

[0028] 2. Obtaining haploid neural stem cells from rhesus monkeys

[0029] The macaque haploid embryonic stem cells in culture were digested into single cells with 0.05% trypsin, resuspended with EB culture medium and cultured in a suspension culture dish. On the first day of differentiation, 10 μM Y-27632 was added to the EB culture medium to reduce cell apoptosis. On the 5th day of differentiation, the EB culture medium was replaced with neural inducti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of bioengineering and discloses a method for acquiring monkey haploid neural stem cells. The method includes: performing optimization culture on the basisof an original common monkey stem cell culture medium, using 0.05% of trypsin / EDTA to treat haploid embryonic stem cells, cloning to a unicellular cell, and using an EB culture medium for suspension culture to form embryoid body balls; starting from the first day of differentiation, adding ROCK inhibitor Y-27632 into the culture medium to inhibit cell apoptosis; on the fifth day, replacing the EBculture medium with a neural induced culture medium; planting EB balls with neuroderm formed preliminarily in a culture dish with base glue paved at the bottom, and adding the neural induced culture medium for differentiation; picking neural garland, and planting in a culture dish with 30ug / ml polyornithine and 3ug / ml laminin paved at the bottom; after culturing for 14 days, performing flow cytometry, and separating haploid cells. By the method, the monkey haploid neural stem cells are acquired in vitro for the first time and have ability of differentiation in a neural downstream direction.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a method for obtaining monkey haploid neural stem cells. Background technique [0002] Because monkey haploid embryonic stem cell lines are difficult to establish (only one research group has established the cell line reported in the literature). Monkey haploid embryonic stem cells have weak differentiation ability, and there will be serious diploidization in the process of haploid neural differentiation. So there is no report to prove that monkey haploid neural stem cells can be obtained by in vitro differentiation. [0003] To sum up, the problem existing in the prior art is that there is a technical gap in obtaining monkey haploid neural stem cell data through in vitro differentiation. Contents of the invention [0004] Aiming at the problems existing in the prior art, the present invention provides a method for obtaining monkey haploid neural stem cells. Since the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/0797
CPCC12N5/0623C12N2500/32C12N2500/44C12N2501/11C12N2501/115C12N2501/235C12N2501/727C12N2533/32C12N2533/52
Inventor 帅领王海松张文豪
Owner NANKAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com