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Peptidoglycan immunopotentiator and its production process

An immunopotentiator and production method technology, applied in pharmaceutical formulations, peptide/protein components, animal feed, etc., can solve the problems of high cost, immature technology, and large difference in product batch quality, and achieve low production cost, Wide application range and high safety effect

Inactive Publication Date: 2006-03-01
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production of peptidoglycan immune enhancer for fish and shrimp is currently limited to small-scale production in the laboratory, which has high costs, large quality differences between product batches, and the overall quality is unstable
In aquaculture, the research and application of immune activators are still in the initial stage in the world, many technologies are immature, and there is a lack of effective technical indicators to evaluate the effect of their application

Method used

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  • Peptidoglycan immunopotentiator and its production process
  • Peptidoglycan immunopotentiator and its production process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The production and application effect of peptidoglycan preparation for the cultivation of prawn seedlings:

[0018] In the embodiment of the present invention, the main equipment conditions used are a 250-liter fermenter with a total volume of 250 liters and a working volume of 240 liters. The components of the fermentation broth used contained 1% glucose, 1% lactose, 1% tryptone, 0.6% yeast extract, 0.6% beef extract, 0.1% Tween-80, 1% Na 2 HPO 4 , 0.6% KH 2 PO 4 and 0.05% MgSO 4 , pH 6.5.

[0019] Take 1 bottle of freeze-dried powder of Bifidobacterium thermophilum stored at -70°C, which is selected from Bifidobacterium thermophilum, Bifidobacterium bifidum, or Bifidobacterium adolescent Bifidobacterium adolescentis, or Bifidobacterium infantis, or Bifidobacterium longum. After being activated by the slant medium, select a single colony and inoculate it in the liquid medium, and after culturing at 37°C for 24 hours, expand the culture to form a seed liquid. When t...

Embodiment 2

[0023] Production and application effect of peptidoglycan immune enhancer for turbot indoor cement pond culture:

[0024] This example is carried out in the same steps as in Example 1, except that the fermentation is terminated after 24 hours of fermentation, nitrogen is introduced into the tank, and the fermentation pressure is reached to two secondary fermentation tanks with a volume of 1000 liters, and each tank is inoculated The content is 12.5%, and the secondary fermentation is carried out. Finally, an immune enhancer with the following physical and chemical parameters is obtained: color: light brown liquid, viscosity: 4.2, specific gravity: 1.030; the immune enhancer contains: peptidoglycan with a relative molecular weight of less than 20KD as an active ingredient according to the W / V ratio: 1.8%, protein with molecular weight less than 20KD: 6%, fat: 0.4%, ribonucleic acid: 0.015%, total solid content: 9%; the rest: water; about 16kg (calculated by the weight of wet ba...

Embodiment 3

[0029] The production and application effect of peptidoglycan preparation for Penaeus vannamei culture production:

[0030] This example is carried out by basically the same steps as in Example 1, except that while the first fermenter is fermenting, the mixed fermentation liquid is injected into another 5-ton fermenter, heated and pressurized to 0.07KPa / 106 Sterilize at ℃ for 20 minutes; turn off all air switches, and connect the nitrogen pipe directly to the air intake end of the air filter; when the pressure in the fermenter drops to 0.02KPa, start feeding nitrogen into the fermenter to maintain positive pressure; the temperature in the fermenter drops When it reaches 37°C, it can be connected to the fermented liquid that has been fermented in the first fermenter; the culture conditions after inoculation, the separation and digestion of the bacteria are the same as in Example 1, and finally obtain the following physical and chemical parameters: color: light brown Liquid, vis...

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Abstract

The peptidoglycan immunopotentiator is light brown, has viscosity of 3.2-5.0 and specific weight of 1.021-1.042, and contains peptidoglycan as active component in 1.5-2.0 wt%, protein in 5-8 wt%, fat in 0.3-0.5 wt%, RNA in 0.01-0.02 wt% and total solids in 8-10 wt% except water. The production process of the immunopotentiator includes the following steps: culturing strain, the first seed culturing and the second stage of seed tank culturing; fermentation in the primary fermenting tank while introducing nitrogen; terminating fermentation after reaching OD value 1.0-1.4; expanding fermentation in n fermenting tanks to obtain target fermented liquid; separation and post-treatment via adding digestive buffering liquid, lysozyime, cysteine, EDTA and papain; and obtaining the required liquid peptidoglycan preparation.

Description

technical field [0001] The invention relates to the improvement of the technology for industrialized production of feed additives for cultured fish and shrimp, in particular to a peptidoglycan immune enhancer and a production method thereof. The invention utilizes the microbial fermentation technology to industrially produce the polysaccharide immune enhancer for fish and shrimp feed. Background technique: [0002] In the current aquaculture, there is a common phenomenon of excessive pursuit of output and neglect of quality. As a result, the breeding density is too high and the water pollution is aggravated, which causes diseases to occur frequently. Especially in recent years, large-scale outbreaks of viral diseases in aquaculture animals have caused huge losses to the aquaculture industry. Therefore, controlling the occurrence of diseases is of great significance to the sustainable development of aquaculture. In the field of aquatic disease prevention and control, antibi...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61P37/04C12P21/00C12P1/00A23K1/16A23K20/147A23K20/153A23K20/158
Inventor 宋晓玲王秀华黄倢
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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