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Bevel solid culture medium and culture method for producing tetracycline through fermentation of streptomyces aureus

A technology of solid medium and Streptomyces aureus, which is applied in the field of biology and medicine, can solve problems such as difficult selection, high quality requirements of origin, and fluctuations in fermentation production, and achieve the effects of fast metabolism, good fermentation level, and stable fermentation process

Inactive Publication Date: 2018-02-02
NINGXIA QIYUAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the defects of the above-mentioned prior art, to provide a method that can make up for the raw material bran in the original formula, which has high requirements on the quality of the origin of wheat, difficult selection, and easy to cause fluctuations in fermentation production, so as to ensure the appearance of the prepared slanted spores Full colonies, pure color, good quality single spores, used for fermentation production, strong cell viability, strong adaptability to various adverse conditions, Streptomyces aureus fermenting and producing tetracycline slant solid medium with stable and improved fermentation level

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1 (repeat three groups)

[0013] 1. Preparation of blank slant

[0014] Slant solid medium composition: bran 2.0%, dipotassium hydrogen phosphate 0.005%, diammonium hydrogen phosphate 0.008%, magnesium sulfate 0.003%, corn steep liquor 0.5%, calcium carbonate 0.003%, agar 1.8%, and the rest is water.

[0015] Preparation of slant solid medium: Dissolve the above materials (except calcium carbonate) and put them in a fixed container, add calcium carbonate to fully dissolve, stir evenly, control the pH at about 7.5, and divide them into 250ml eggplant-shaped bottles, each The bottle contains 40-60ml. Sterilize, control the sterilization temperature at 117-121°C, pressure at 0.008-0.012MPa, sterilize for 20-25 minutes, spread it into a slope and cool it for later use.

[0016] 2. Incline inoculation and culture

[0017] Take 1 original strain of Streptomyces aureus, absorb 0.2ml and pour it into sterilized purified water under aseptic conditions to make a bacter...

Embodiment 2

[0025] 1. Preparation of blank slant

[0026] The composition of the solid medium on the slope: 2.5% bran, 0.006% dipotassium hydrogen phosphate, 0.01% diammonium hydrogen phosphate, 0.005% magnesium sulfate, 0.8% corn steep liquor, 0.003% calcium carbonate, 2.0% agar, and the rest is water.

[0027] Preparation of slant solid medium: Dissolve the above materials (except calcium carbonate) and put them in a fixed container, add calcium carbonate to fully dissolve, stir evenly, control the pH at about 7.5, and divide them into 250ml eggplant-shaped bottles, each The bottle contains 40-60ml. Sterilize, control the sterilization temperature at 117-121°C, pressure at 0.008-0.012MPa, sterilize for 20-25 minutes, spread it into a slope and cool it for later use.

[0028] 2. Incline inoculation and cultivation are the same as in Example 1.

[0029] 3. Seed culture and fermentation culture are the same as in Example 1.

[0030] 4. The above experimental group was repeated for three...

Embodiment 3

[0033] 1. Preparation of blank slant

[0034] Slope solid medium composition: bran 2.5%, dipotassium hydrogen phosphate 0.008%, diammonium hydrogen phosphate 0.013%, magnesium sulfate 0.006%, corn steep liquor 1.0%, calcium carbonate 0.003%, agar 2.2%, and the rest is water.

[0035] Preparation of slant solid medium: Dissolve the above materials (except calcium carbonate) and put them in a fixed container, add calcium carbonate to fully dissolve, stir evenly, control the pH at about 7.5, and divide them into 250ml eggplant-shaped bottles, each The bottle contains 40-60ml. Sterilize, control the sterilization temperature at 117-121°C, pressure at 0.008-0.012MPa, sterilize for 20-25 minutes, spread it into a slope and cool it for later use.

[0036] 2. Incline inoculation and cultivation are the same as in Example 1.

[0037] 3. The cultivation of seeds and fermentation is the same as in Example 1.

[0038] 4. The above experimental group was repeated for three groups, and t...

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Abstract

The invention relates to a bevel solid culture medium and culture method for producing tetracycline through fermentation of streptomyces aureus. The culture medium is prepared from, by weight, 2.0-2.5% of bran, 0.005-0.008% of dipotassium phosphate, 0.008-0.013% of diammonium hydrogen phosphate, 0.003-0.006% of magnesium sulfate, 0.5-1.0% of corn steep liquor, 0.003% of calcium carbonate, 1.8-2.5%of agar and the balance water. By changing the raw material composition in the streptomyces aureus bevel solid culture medium, the defect that culture quality fluctuation is caused by raw material quality fluctuation in the original formula is overcome, it is ensured that the prepared bevel spores are stable in both appearance and culture activity, and the fermentation level is also stably improved.

Description

technical field [0001] The invention belongs to the technical field of biology and medicine, and in particular relates to a slant solid medium and a culture method for fermenting and producing tetracycline by Streptomyces aureus. Background technique [0002] Tetracycline is a class of macrolide antibiotics, which is mainly fermented and produced with Streptomyces aureus as the production strain. The traditional fermentation method is to use freeze-dried tube bacteria to prepare a bacterial suspension, directly inoculate it on a slant solid medium for cultivation, and produce a large number of spores, then inoculate the spore suspension into the seed medium, and finally inoculate the well-growing The seed liquid is aseptically inoculated in the fermentation medium for fermentation production. For a long time, as the medium for Streptomyces aureus slant culture, bran is used as the main carbon source and trace element supply, inorganic phosphate is used as the supply of phos...

Claims

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Application Information

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IPC IPC(8): C12P29/00C12N1/20C12R1/49
CPCC12N1/20C12P29/00
Inventor 章惠霞孙瑞君张志郭惠芬
Owner NINGXIA QIYUAN PHARMA
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