7results about How to "High bacterial activity" patented technology

The invention belongs to the technical field of functional microorganism screening and application, and particularly relates to a thermophilic halogenobacter nounudi and application thereof. The halogenobacillusrupiensis is halogenobacillusrupiensis S2-5, the halogenobacillusrupiensis S2-5 is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the halogenobacillusrupiensis S2-5 is CCTCC NO (China Center for Type Culture Collection): M 20241447. The strain S2-5 provided by the invention is screened out under high-temperature composting, and has the advantages of good thermal stability, no inhibition of metabolic activity by the temperature of high-temperature composting, and strong adaptability. The compost inoculant obtained by solid state fermentation of the anaerobic bacillus tubingensis S2-5 strain provided by the invention has the characteristics of being easy to preserve and transport, high in strain activity and the like. And the fertilizer prepared from the composting inoculant can shorten the composting period when the composting inoculant is applied to high-temperature composting treatment of organic solid wastes.

The invention belongs to the technical field of fermentation engineering, and particularly relates to lactobacillus reuteri with high beta-glucosidase activity and application of the lactobacillus reuteri in fermentation of sea-buckthorn juice. The preservation number of the strain is CGMCC NO: 33026. The strain is an adaptive evolutionary probiotic, can improve the activity and tolerance of the probiotic, has a good application effect in fermentation of sea-buckthorn juice, can maintain high bacterial activity, and has a good application prospect in fermentation of sea-buckthorn juice. According to the sea-buckthorn juice, the viable count of the sea-buckthorn juice is kept at 9.12-9.37 log (cfu / mL), compared with unfermented sea-buckthorn juice, the fermented sea-buckthorn juice has higher total phenol content and total flavone content, the antioxidant activity of the fermented sea-buckthorn juice is improved, the total phenol content can reach 545 mg / 100 mL and is increased by 95% compared with the unfermented sea-buckthorn juice, meanwhile, the method is a safe method without introduction of exogenous genes, can be applied to food, and has wide application prospects. Good application prospects are realized.

The invention discloses a growth-promoting rhizosphere strain W16 and application thereof, the growth-promoting rhizosphere strain W16 has the preservation number of CGMCC No.31198 and has the capability of promoting plant growth, experiments prove that the strain W16 has the growth-promoting characteristics of IAA production, siderophore production, phosphate solubilization, nitrogen fixation and polyglutamic acid production at the same time, the comprehensive growth-promoting capability is high, and when the concentration of tryptophan in a culture medium is increased, the growth-promoting rhizosphere strain W16 can promote the growth of plants. The IAA generation capability of the strain W16 is enhanced, and the strain W16 has the phosphorus dissolving and nitrogen fixing capabilities at the same time; the relative content of the iron-producing carrier of the strain W16 is 65.52%; when the pH value is equal to 9, the strain W16 still has relatively strong bacterial activity and can grow relatively well; the strain W16 has a high polyglutamic acid production capability reaching 207.16 [mu] g / mL, a tobacco seedling pot experiment is carried out, and the result proves that the strain W16 can promote the growth of tobacco under saline-alkali stress.

The invention discloses a vegetable protein fermentation production process, belongs to the field of vegetable protein deep processing, and aims to solve the problems of low protein hydrolysis degree, low small molecule peptide content, low survival number of probiotics and poor product stability of a traditional vegetable protein fermentation process. The process comprises the following steps: raw material pretreatment: performing ultrasonic-assisted crushing to prepare a suspension and adjusting the pH value; ultrasonic-assisted enzymolysis: adding a compound enzyme preparation and carrying out ultrasonic treatment; enzyme deactivation; fermentation strain preparation: culturing aspergillus oryzae, lactobacillus plantarum and streptococcus thermophilus composite strains; performing segmented temperature-controlled fermentation: performing fermentation at 28-30 DEG C for 12-16 hours, and then performing fermentation at 33-35 DEG C for 8-12 hours; and post-processing. Through the synergistic effect of ultrasonic-assisted crushing, ultrasonic-assisted enzymolysis and segmented temperature-controlled fermentation, the protein hydrolysis degree is larger than or equal to 45%, the proportion of small molecule peptide is higher than 25%, the survival number of probiotics is larger than or equal to 1 * 10 < 6 > CFU / g, the nutritive value and fermentation efficiency of the product are remarkably improved, and the process is stable and easy to industrialize.

The present invention relates to a fermentation medium capable of increasing the yield of avermectin, and belongs to the technical field of microbial fermentation medium. The fermentation medium of the present invention comprises a basic culture solution and a replenishing solution, wherein the basic culture solution comprises 12-15 g / L of peanut meal extract I, 20-25 g / L of peanut meal biochar, 20-30 g / L of gelatinized potato starch, 8-10 g / L of yeast extract powder, 0.3-0.5 g / L of magnesium sulfate heptahydrate, 0.3-0.5 g / L of diammonium hydrogen phosphate, and 1-1.2 g / L of calcium lactate; the replenishing solution comprises 15-18 g / L of peanut meal extract II, 10-12 g / L of erythritol, 3-5 g / L of potassium chloride, 10-12 g / L of ammonium sulfate, and 5-8 g / L of brown algae polysaccharide. When the culture medium provided in the present invention is used for avermectin fermentation production, the yield of avermectin can be significantly increased, and the product has certain stability.

The invention discloses a mother strain culture medium for enhancing vigor of a pilose antler mushroom strain. The mother strain culture medium is prepared from the following raw materials in parts by weight: 200 parts of potatoes, 20 parts of glucose, 12 parts of agar powder, 50 parts of kiwi-fruit juice and 1300 parts of water. Or the mother strain culture medium comprises the following raw materials in parts by weight: 150-250g of potatoes, 15-25g of glucose, 10-15g of agar powder and 1200-1400ml of water, and the content of vitamin C in the culture medium is 0.4-0.6 mol. According to the mother strain culture medium, the strain activity of mother strains can be effectively improved, the cultivation period of the pilose antler mushrooms can be shortened in the further cultivation process, the finished product quality and yield of the pilose antler mushrooms are improved, and the good practical application and popularization value is achieved.

The invention relates to the technical field of compound fertilizers, and provides a high-temperature-resistant bacteria-containing compound fertilizer and a preparation method thereof.The preparation method comprises the following steps that nanoscale freeze-dried bacterial powder is prepared from bacillus, bacillus stearothermophilus and rhodopseudomonas palustris; mixing the nano TiO2 modified SiO2 composite aerogel, diatomite, the modified charcoal, sepiolite and pullulan to prepare a microbial inoculum protection composite carrier; and mixing the freeze-dried bacterial powder with a carrier, and drying to obtain the micron-sized capsule bacterial agent. Uniformly mixing a reinforcing agent, a plasticizer and a stabilizer to prepare a performance regulator; the preparation method comprises the following steps: melting choline chloride-containing urea and monoammonium phosphate to prepare molten slurry; mixing the molten slurry with a compound fertilizer to form uniform slurry; and mixing the slurry with a micron-sized capsule microbial inoculum and a performance regulator, and granulating to obtain the high-temperature-resistant bacterium-containing compound fertilizer. By optimizing the adaptability of a microbial agent embedding carrier and a melting system, the technical bottleneck of conflict between high-temperature inactivation of the microbial agent and physical properties of a product is solved.