70results about How to "High bacterial activity" patented technology

The invention relates to a complex microorganism fungicide for treating animal manure and a preparation method and an application thereof. The adopted technical scheme is that the complex microorganism fungicide consists of saccharomyces cerevisiae, powdery pichia, bacillus subtilis, bacillus cereus, bacillus megaterium and lactobacillus plantarum. Microbial organic fertilizer can be prepared by treating the animal manure with the complex microorganism fungicide. The complex microorganism fungicide for treating the animal manure prepared by the invention has the advantages of easy obtainment of raw materials and simple manufacturing method. Preparing the microbial organic fertilizer by mixing the complex microorganism fungicide for treating the animal manure with the animal manure, organic materials and cornmeal not only can treat the animal manure, but also effectively utilizes rural wastes; and the prepared microbial organic fertilizer can not pollute crops and cultivated land because no chemical substance exists in the prepared microbial organic fertilizer.

The invention relates to a method capable of improving methane yield in a sludge anaerobic digestion process and enhancing heavy metal stabilization process. According to the method, corn stalk is utilized as a raw material to prepare stalk activated carbon under nitrogen protection and pyrolysis temperature of 550 DEG C; the stalk activated carbon is utilized as a carrier to react with a KMnO4 solution to finally obtain a nano MnO loaded activated carbon function material; in a sludge anaerobic digestion acid production stage, the nano MnO loaded activated carbon function material is added into an anaerobic reaction detection according to a proportion, cumulative gas production of methane in the sludge anaerobic digestion process is obviously improved, free-state heavy metal in the sludge can be effectively adsorbed and processed in a complexation mode at the same time, biological validity and mobility of the heavy metal are reduced, and the heavy metal stabilization process is enhanced. The method disclosed by the invention can be applied to residual sludge anaerobic digestion of urban sewage treatment plants, effectively improves the cumulative gas production of the methane, reduces biological validity of the heavy metal in the sludge anaerobic digestion process and enhances the heavy metal stabilization process.

The preparation process of acidophilic lactobacillus milk tablet adopts inducing mutation breeding method to breed high temperature mutant acidophilic lactobacillus strain, i. e., acidophilic lactobacillus LA201 strain. Meanwhile, matter capable of lowering the death rate of acidophilic lactobacillus is extracted from the Chinese herbal medicine extract liquid and microbe culture liquid. The breeding of high temperature acidophilic lactobacillus strain and the protecting agent make it possible to produce acidophilic lactobacillus milk tablet in available milk powder producing high temperature spray drying apparatus and conventional tabletting.

The invention relates to a bio-floating bed and an on-site three-dimensional bio-remediation method for a micro-polluted water body. The bio-floating bed relating in the invention comprises a floating bed body, and a combined biological stuffing is arranged on the floating bed body. Also, plants can be grown on the floating bed body. Efficient microflora obtained by enriching and domesticating bottom sediment of a to-be-treated water body is suspended on the used combined biological stuffing, and has the advantages of being high in biological activity, strong in adaptability and good in treatment effect. The invention further relates to an on-site three-dimensional bio-remediation method for the micro-polluted water body. The method comprises the steps of: covering the bottom sediment of the micro-polluted water body with about 1-2cm coal ash, then spraying the film-suspended biological stuffing on the coal ash by about 1-2cm; then covering the water surface of the micro-polluted water body with the bio-floating bed, wherein the coverage area takes up 10%-20% of the water surface area of the micro-polluted water body. Through the remediation method, the micro-polluted water body can be continuously purified for three to four months.

Novel conjugates composed of a saccharide-containing moiety (e.g., aminoglycosides) covalently linked to a moiety containing two or more basic amino acid residues (e.g., a polyarginine) and processes of preparing same are disclosed. Further disclosed are pharmaceutical compositions containing these conjugates and uses of these conjugates as antiviral and antibacterial agents.

The invention discloses a process method for treating dehydroacetic acid mixed wastewater. The method comprises: preparing a compound bacterial agent, wherein pseudomonas, acinetobacter and bacillus are cultured and fermented respectively, fermentation broths obtained through fermentation are adsorbed to prepare bacterial powders, and the bacterial powders are mixed according to the mass ratio of20%-30% of pseudomonas, 10%-30% of acinetobacter and 40%-70% of bacillus to form a compound bacterial agent; pre-treating wastewater, wherein wastewater enters an adjusting tank and is diluted to adjust the COD of the wastewater to 1300-1700 mg/L and the pH value to 6.5-7.0; and mixing the dehydroacetic acid wastewater, a biological synergistic nutritional agent and water according to a ratio of (1-2):(1-2):(6-8) to form mixed water, adding a composite strain into the mixed water, mixing and activating at 20-35 DEG C by controlling DO at 1-6 mg/L in an aeration manner and the pH value at 6-9,and reacting for 5-50 hours to finish activation, wherein the mass ratio of the compound bacterial agent to the mixed water is (0.5-10):110. The invention also discloses an application of the method in treatment of dehydroacetic acid wastewater. According to the invention, the wastewater treatment efficiency can be improved, the wastewater treatment cost is reduced, and the COD removal rate is increased.

The invention discloses a preservation method of a liquid bacterial agent for sewage treatment. The preservation method comprises the following main steps: 1) regulating the pH value of the liquid bacterial agent to 6-6.5 by using a buffer solution; 2) removing dissolved oxygen from the liquid bacterial agent by using nitrogen; 3) adding a deoxidizer and a bacterium inhibitor; 4) covering with liquid paraffin, and sealing for preserving. By adopting the preservation method, 12 months later, the viable bacteria amount and the degrading activity can respectively reach 65% and 75% above.

The invention discloses a lactic acid bacteria freeze-drying protecting agent and application thereof. The protecting agent is composed of a skimmed milk powder solution and an ectoine solution, content of skimmed milk powder is 5-30wt% of the protecting agent, and content of ectoine is 0.08-0.5wt% of the protecting agent. Different from conventional protecting agents having complex formulas, the freeze-drying protecting agent is simple in formula composition, and bacteria activity after freeze-drying is higher than that of other commonly-used protecting agents, so that viable count is guarantee. Experimental study finds that the freeze-drying protecting agent plays an unexpected role in protecting lactic acid bacteria, the lactic acid bacteria after being mixed with the protecting agent are freeze-dried, obtained bacterial powder is high in activity and stability, and freeze-drying has little impact on the lactic acid bacteria, so that long-time storage and transportation of the bacterial powder are facilitated.

The invention provides a selenium-enriched biofertilizer and a preparation method of the selenium-enriched biofertilizer by complexing active bacteria and nano-selenium elements. According to the selenium-enriched biofertilizer, by means of chemical and physical mutagenesis and the like, the active bacteria and the nano-selenium elements are complexed through a reasonable coordination mode. A new strain contains the nano-selenium elements which have low toxicity and good biological activity, thereby the strain can be fully absorbed by human body, and the requirement of the human body for the selenium can be met.

The invention relates to a direct-injection old yeast steamed bun fermentation agent capable of improving steamed bun flavor. The direct-injection old yeast steamed bun fermentation agent is composedof short lactobacillus powder, lactobacillus plantarum powder, active dry yeast and fungal amylase in the mass ratio of 0.5-1:0.5-1:0.5-2:0.002-0.01. The fermentation agent can directly replace the traditional old yeast fermentation agent, and is directly used for dough fermentation and steamed bun making to improve the fermentation speed of the traditional old yeast and give steamed buns rich unique flavor. The fermentation agent is simple in preparation procedure, high in vitality and stable in quality, facilitates quality monitoring and can be used for industrial production of the steamed buns.

The invention discloses fermented fruit and vegetable juice containing rich black corn anthocyanin as well as a preparation method and device thereof. The fermented fruit and vegetable juice is prepared from the following raw materials: fruit and vegetable juice, black corn anthocyanin extract, a sweetener, a stabilizing agent, a compound fermentation agent and distilled water; the preparation method comprises the following steps: S1: chopping fruits and vegetables, and mixing a complex enzyme, fruit and vegetable blocks and de-ionized water; pulping and filtering to obtain raw fruit and vegetable juice; S2: decompressing and concentrating the raw fruit and vegetable juice; adding a compound fermentation agent and carrying out four phases of fermented to obtain fermented fruit and vegetable juice; S3: adding the black corn anthocyanin into the fermented fruit and vegetable juice; carrying out reverse osmosis dehydration treatment and high-pressure homogenization treatment to obtain homogenized fruit and vegetable juice; S4: sterilely filling the homogenized fruit and vegetable juice to obtain the fermented fruit and vegetable juice containing rich black corn anthocyanin. Totally, the prepared fermented fruit and vegetable juice has high black corn anthocyanin content; the loss of nutrient components of the fruit and vegetable juice is less and the nutrient value is high.

The invention discloses multifunctional fermentation equipment which is characterized in that a first fermentation tank is connected with a second fermentation tank; stirring rods are arranged in the fermentation tanks and are connected with a variable-speed motor which is connected with a motor; the motor is connected with an electrical appliance switch; and brackets and filter screens are arranged in the fermentation tanks. The structure of the first fermentation tank can be identical with that of the second fermentation tank; and the second fermentation tank can also be used for a fermenting and filtering and pH value adjusting tank. Multiple functions can be realized by one tank, and the multifunctional fermentation equipment is used for fermenting feed for feeding pigs, chickens, ducks, cattle, sheep and poultry. The multifunctional fermentation equipment has the advantages of simple structure, easiness in manufacture and convenience in mounting and using; the fermented mixed strain is high in activity, strong in vitality, low in cost and proper in the pH value; the feed prepared from fermented materials is popular among livestock and can be used for promoting the adjustment in the bodies of the livestock and enabling the livestock to quickly grow; and the multifunctional fermentation equipment can also be used for spraying pigsties and mews, deodorizing, eradicating odor and deinsectizing. The multifunctional fermentation equipment can be used for fermenting single strain or various mixed strains.

The invention provides an agricultural probiotic agent produced by harmless solid fermentation of traditional Chinese medicine waste residues and a preparation method of the agricultural probiotic agent. Peanut shells, rice husks, corn steep liquor, soybean meal, magnesium sulfate, ammonium sulfate, glucose, sodium chloride and traditional Chinese medicine residues are used as base materials to bereasonably proportioned; solid fermentation of bacillus subtilis, bacillus licheniformis, bacillus megatherium, bacillus mucilaginosus, lactic acid bacteria, saccharomycetes, and fungal spore powderis carried out; the thallus number of bacillus subtilis and bacillus licheniformis reaches more than 10 billion cfu/g; the thallus number of bacillus megatherium and bacillus mucilaginosus reaches more than 5 billion cfu/g; the thallus number of lactic acid bacteria reaches more than 10 billion cfu/g; the thallus number of saccharomycetes reaches more than 5 billion cfu/g; the thallus number of fungal spore powder reaches more than 5 billion cfu/g. The preparation method has the advantages that the problem that environment pollution caused by the traditional Chinese medicine residues is avoided, and the low-cost probiotic agent is developed.

The invention discloses formula milk powder with the functions of lowering three highs and a preparation method thereof, and belongs to the technical field of milk powder processing. The formula milkpower is prepared from, by mass, 100 parts of skim milk, 0.01-0.1 part of compound lactobacillus, 1-5 parts of jerusalem artichoke powder, 0.1-0.5 part of oligosaccharides, 1-5 parts of instant soybean powder, 0.01-0.1 part of hawthorn powder, 0.001-0.01 part of conjugated linoleic acid, 0.001-0.01 part of linseed oil, 0.001-0.01 part of walnut oil, and 0.001-0.005 part of L-carnitine. The formulaof the milk powder adopts organic compounding of multiple-source functional factors having the functions of reducing blood lipid, blood pressure, blood sugar and the like. The preparation method adopts a wet process for production with different raw materials added in stages and lactic acid bacteria added after embedding. According to the formula milk powder and the preparation method, the healtheffect of added nutrients can be maximized, the sensory performance is good, storage resistance is good, the bacterial activity is high and the formula milk powder has remarkable effects on loweringthe three highs.

The invention discloses a method for improving the aroma of Cantonese high-salt dilute fermented soy sauce. The method includes the steps that S1, a refrigerated salt-tolerant aroma former mother strain is inoculated into a mother-strain flask culture medium for culture and then inoculated into a production flask culture medium for culture to obtain a salt-tolerant aroma former stock strain; S2, the salt-tolerant aroma former stock strain after treatment in S1 is inoculated into a seed tank culture medium for culture and then inoculated into a fermentation tank culture medium for culture untilthe viable count of the culture solution reaches 108 or above; S3, the pH of the culture solution after treatment in S2 is regulated and maintained, and standing is carried out, wherein a ventilatedstate is maintained in the standing process; S4, the strain in the culture solution after treatment in S3 is added into soy sauce which is being fermented. By means of the method, it can be ensured that the salt-tolerant aroma former added in the soy sauce fermentation process has high strain activity, and thus the aroma of the prepared soy sauce is effectively improved.

The invention belongs to the technical field of edible fungi strain preparation and relates to a preparation method of an agaricus bisporus liquid strain. The preparation method comprises propagation expanding culture using a slant culture medium, first-level and second-level culture media and a fermentation culture medium; the formulas of the culture mediums provided by the invention can provide the nutritional ingredients required in the growth process of the agaricus bisporus strain, the growth cycle of the agaricus bisporus liquid strain is shortened, and the strain activity is enhanced; according to the invention, by adding a little green tea extract, the generation of mould, sundry fungi and the like can be effectively prevented in the growth process of agaricus bisporus; and moreover, the growth of agaricus bisporus strain is promoted, the mycelia grow fast, the quality is good, and the purity is high.

The invention relates to the technical field of edible mushroom cultivation, in particular to a method for preparing a lucid ganoderma culture medium and lucid ganoderma mycelia. The lucid ganoderma culture medium comprises the following raw material components in parts by weight: 50-150 parts of bits of wood, 10-40 parts of wheat bran, 0.1-10 parts of gypsum powder, 0.1-10 parts of sugar and 0.0001-0.001 part of vitamin B2. The method for preparing the lucid ganoderma culture medium and the lucid ganoderma mycelia, which is provided by the invention, is scientific and reasonable in raw material formula matching, the lucid ganoderma mycelia obtained through inoculation of the culture medium are relatively high in activity and relatively high in inoculation rate, and furthermore the planting yield of the lucid ganoderma is increased. Meanwhile, the method for preparing the lucid ganoderma culture medium and the lucid ganoderma mycelia, which is provided by the invention, has the advantages of being high in efficiency, low in cost, simple to operate and good in practicability.

The invention relates to a method for enhancing anaerobic ammonia oxidizing bacteria culture and improving bacteria activity by using ferrous sulfide, which comprises the following steps: adding ferrous sulfide particles and anaerobic granular sludge into an up-flow anaerobic sludge bed reactor, wherein the addition amount of the ferrous sulfide particles is 1-10 g/L to-be-treated wastewater containing ammoniacal nitrogen and nitrite nitrogen; and conducting stirring under the anaerobic dark condition of 30-34 DEG C to enable anaerobic ammonia oxidation sludge in the reactor to fully react with pollutants, and adjusting the water inlet flow rate to enable the hydraulic retention time (HRT) to be 24-48 hours. By adding ferrous sulfide, culture of anaerobic ammonia oxidizing bacteria can beaccelerated, the starting time of the reactor is shortened, the activity of the anaerobic ammonia oxidizing bacteria is improved, and obtained particles are more compact and better in stability. The method disclosed by the invention is simple in process and mild in reaction conditions, and can be carried out under normal anaerobic ammonia oxidation reaction conditions; and the method is safe and harmless to the environment and convenient for engineering application.

The invention discloses a preparation method of instant-brew-yogurt milk powder, and belongs to the technical field of food processing. The method comprises the steps that: (1) white granulated sugar is added into raw material milk; sterilization is carried out after homogenization; and concentration is carried out, such that concentrated milk is obtained; (2) activated yogurt fermentation bacteria are inoculated into an enrichment culturing liquid; after one time of culturing, centrifugation is carried out and the supernatant is removed; enrichment culturing liquid is added into sediment bacterial sludge, and secondary culturing is carried out; centrifugation is carried out, such that the supernatant is removed; the concentrated milk with a volume of 1/3 of that of the enrichment culturing liquid is added into the bacterial sludge; homogenization is carried out, such that high-bacteria concentrated yogurt fermentation broth is obtained; and (3) the concentrated milk is mixed with the high-bacteria concentrated yogurt fermentation broth; homogenization is carried out, and spray drying is carried out, such that milk powder used for processing instant-brew yogurt is obtained. The milk powder has the advantages of simple production process, low cost, environment friendliness, and suitability for large-scale industrial productions. The prepared product is convenient for storage, transportation, and drinking. When the instant-brew yogurt is to be prepared, warm boiled water is added into the milk powder, and fermentation is carried out under maintained temperature, such that the yogurt can be prepared. The yogurt is nutritious, healthy, safe, and convenient. The yogurt is suitable for people of all ages.