Mother strain culture medium for enhancing vigor of pilose antler mushroom strain

Abstract

The invention discloses a mother strain culture medium for enhancing vigor of a pilose antler mushroom strain. The mother strain culture medium is prepared from the following raw materials in parts by weight: 200 parts of potatoes, 20 parts of glucose, 12 parts of agar powder, 50 parts of kiwi-fruit juice and 1300 parts of water. Or the mother strain culture medium comprises the following raw materials in parts by weight: 150-250g of potatoes, 15-25g of glucose, 10-15g of agar powder and 1200-1400ml of water, and the content of vitamin C in the culture medium is 0.4-0.6 mol. According to the mother strain culture medium, the strain activity of mother strains can be effectively improved, the cultivation period of the pilose antler mushrooms can be shortened in the further cultivation process, the finished product quality and yield of the pilose antler mushrooms are improved, and the good practical application and popularization value is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of velvet mushroom cultivation, and in particular to a mother culture medium for enhancing the vitality of velvet mushroom strains. Background Art

[0002] Pilosa velvet mushroom has thick flesh, crispy and smooth taste, rich in nutrients such as protein, polysaccharides, vitamins and minerals, and has multiple health benefits such as anti-oxidation, anti-inflammatory, and immune regulation, and is deeply loved by consumers. With the in-depth research and promotion of edible fungi, people's awareness of ilvesa velvet mushroom is gradually increasing, market demand continues to increase, and the cultivation of ilvesa velvet mushroom as a whole shows a trend of steady growth year by year.

[0003] The cultivation cycle of velvet mushroom is relatively long, and it generally takes about 100 days to harvest the finished product. The cultivation of velvet mushroom generally adopts two methods: bottle cultivation or bag cultivation. The velvet mushroom strains are connected to the prepared culture medium, and the appropriate temperature, humidity, carbon dioxide, light and other conditions are controlled to promote the growth and development of velvet mushroom. In this process, the cultivation of velvet mushroom mother strains is extremely critical. In the prior art, the velvet mushroom mother strain culture medium materials mostly include potatoes, agar, etc. The culture medium has a single nutrient component, the strain vitality is low during the cultivation process, the strain is easy to degenerate, and the culture cycle after inoculation is long, which affects the quality and yield of edible fungi. Therefore, the present invention aims to develop a mother strain culture medium and a culture medium preparation method for enhancing the vitality of velvet mushroom strains to better meet actual needs. Summary of the invention

[0004] The technical problem solved by the present invention is to provide a mother culture medium for enhancing the vitality of Pleurotus eryngii strains, so as to solve the problems in the above-mentioned background technology.

[0005] The technical problem solved by the present invention is achieved by adopting the following technical solutions:

[0006] A mother culture medium for enhancing the vitality of velvet antler mushroom strains, the mother culture medium consists of potatoes, glucose, agar powder, adjuvants and a proper amount of water.

[0007] Furthermore, the adjuvant is kiwi juice or vitamin C.

[0008] Furthermore, the mother culture medium comprises the following raw materials in parts by weight: 150-250 parts of potatoes, 15-25 parts of glucose, 10-15 parts of agar powder, 45-55 parts of kiwi juice, and 1200-1400 parts of water.

[0009] Furthermore, the mother culture medium comprises the following raw materials in parts by weight: 200 parts of potatoes, 20 parts of glucose, 12 parts of agar powder, 50 parts of kiwi juice, and 1,300 parts of water.

[0010] Furthermore, the kiwifruit juice is obtained by squeezing fresh kiwifruit and then filtering it.

[0011] Furthermore, the mother culture medium comprises the following raw materials in parts by weight: 150-250 g of potato, 15-25 g of glucose, 10-15 g of agar powder, and 1200-1400 ml of water. The content of vitamin C in the culture medium is 0.4-0.6 mol.

[0012] Furthermore, the mother culture medium comprises the following raw materials in parts by weight: 200 g potato, 20 g glucose, 12 g agar powder, and 1300 g water. In addition, the content of vitamin C in the culture medium is 0.5 mol.

[0013] Beneficial effects: The mother culture medium for enhancing the vitality of Pleurotus eryngii strains of the present invention can effectively improve the vitality of the mother culture strains, and after inoculation, it can effectively shorten the cultivation period of Pleurotus eryngii, reduce the cultivation cost, and improve the finished product quality and yield of Pleurotus eryngii. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 The mycelium observation picture and the weighing picture of the velvet antler mushroom product in Example 1 are shown.

[0015] Figure 2 This is the mycelium observation picture and the Pleurotus antler product weighing picture of Example 2.

[0016] Figure 3 This is the mycelium observation picture of Control Example 1 and the weighing picture of Pleurotus eryngii product.

[0017] Figure 4 This is the mycelium observation picture of Control Example 2 and the weighing picture of Pleurotus eryngii product.

[0018] Figure 5 This is the mycelium observation picture of Control Example 3 and the weighing picture of Pleurotus eryngii product.

[0019] Figure 6 This is the mycelium observation picture of Control Example 4 and the weighing picture of the Pleurotus eryngii product.

[0020] Figure 7 This is the mycelium observation picture of Control Example 5 and the weighing picture of Pleurotus ostreatus product. DETAILED DESCRIPTION

[0021] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific embodiments.

[0022] Example 1

[0023] The mother culture medium for enhancing the vitality of Pleurotus eryngii strains described in this embodiment comprises the following raw materials in parts by weight: 200 g potato, 20 g glucose, 12 g agar powder, 50 ml kiwi juice, and 1300 ml water. The kiwi juice is obtained by squeezing fresh kiwi juice and filtering it.

[0024] Mix the raw materials of the mother culture medium and divide them into the culture medium. Sterilize the culture medium with high pressure steam at 121°C for 20 minutes and then inoculate the activated mother culture mycelium on the culture medium after cooling. Then, culture it at a constant temperature of 25°C in the dark for recovery. After the mycelium grows on the culture medium, draw a line on the culture medium to mark it and continue to culture for 20 days. During this period, draw a line every 4 days to observe and record the growth conditions of these 20 days, including growth potential, color, average growth rate, etc.

[0025] Weigh the raw materials required for preparing 1000 bags of conventional culture medium for cultivating velvet antler mushrooms according to the proportion, put them into the blender and mix them evenly, then add water and stir for 45 minutes until the water content reaches 65%, adjust the pH to 9.2, and bag them. The polypropylene bag used for bagging has a circumference of 38cm and a length of 36cm. The height after bagging is 19CM and the weight is 1500g.

[0026] After bagging, sterilize at 123℃ for 120min, and inoculate after cooling. After inoculation, culture in a dark room at 24℃ and 75% humidity for about 70 days. After the mycelium is white and strong, move it to the scratching room, remove the ring cover, and then move it to the fruiting room to enter the fruiting stage. Strict fruiting management is required during the fruiting stage, with the temperature controlled at 17.5℃, humidity at 85%, light at 100 lux, and carbon dioxide at 2500ppM. Harvest after 28 days.

[0027] In this embodiment, the mycelium observation and the weighing of the velvet antler mushroom product are as follows: Figure 1 shown.

[0028] Example 2

[0029] The mother culture medium for enhancing the vitality of Pleurotus eryngii strains described in this embodiment includes the following raw materials in parts by weight: 200g potato, 20g glucose, 12g agar powder, 1300ml water, and also includes 0.5mol of vitamin C.

[0030] Mix the raw materials of the mother culture medium and divide them into the culture medium. Sterilize the culture medium with high pressure steam at 121°C for 20 minutes and then inoculate the activated mother culture mycelium on the culture medium after cooling. Then, culture it at a constant temperature of 25°C in the dark for recovery. After the mycelium grows on the culture medium, draw a line on the culture medium to mark it and continue to culture for 20 days. During this period, draw a line every 4 days to observe and record the growth conditions of these 20 days, including growth potential, color, average growth rate, etc.

[0031] Weigh the raw materials required for preparing 1000 bags of conventional culture medium for cultivating velvet antler mushrooms according to the proportion, put them into the blender and mix them evenly, then add water and stir for 45 minutes until the water content reaches 65%, adjust the pH to 9.5, and bag them. The polypropylene bag used for bagging has a circumference of 38cm and a length of 36cm. The height after bagging is 19CM and the weight is 1500g.

[0032] After bagging, sterilize at 123℃ for 120min, and inoculate after cooling. After inoculation, culture in a dark room at 25℃ and 78% humidity for about 70 days. When the mycelium is white and strong, move it to the scratching room, remove the ring cover, and then move it to the fruiting room to enter the fruiting stage. Strict fruiting management is required during the fruiting stage, with the temperature controlled at 18℃, humidity at 80%, light at 100 lux, and carbon dioxide at 3000ppM. Harvest after 28 days.

[0033] In this embodiment, the mycelium observation and the weighing of the velvet antler mushroom product are as follows: Figure 2 shown.

[0034] Comparative Example 1

[0035] The mother culture medium for enhancing the activity of Pleurotus eryngii strains in this control example comprises the following raw materials in weight: 200g potato, 20g glucose, 12g agar powder, and 1300ml water. The kiwi juice is obtained by squeezing fresh kiwi juice and filtering it.

[0036] Mix the raw materials of the mother culture medium and divide them into the culture medium. Sterilize the culture medium with high pressure steam at 121°C for 20 minutes and then inoculate the activated mother culture mycelium on the culture medium after cooling. Then, culture it at a constant temperature of 25°C in the dark for recovery. After the mycelium grows on the culture medium, draw a line on the culture medium to mark it and continue to culture for 20 days. During this period, draw a line every 4 days to observe and record the growth conditions of these 20 days, including growth potential, color, average growth rate, etc.

[0037] Weigh the raw materials required for preparing 1000 bags of conventional culture medium for cultivating velvet antler mushrooms according to the proportion, put them into the blender and mix them evenly, then add water and stir for 45 minutes until the water content reaches 65%, adjust the pH to 9.5, and bag them. The polypropylene bag used for bagging has a circumference of 38cm and a length of 36cm. The height after bagging is 19CM and the weight is 1500g.

[0038] After bagging, sterilize at 123℃ for 120min, and inoculate after cooling. After inoculation, culture in a dark room at 25℃ and 78% humidity for about 70 days. When the mycelium is white and strong, move it to the scratching room, remove the ring cover, and then move it to the fruiting room to enter the fruiting stage. Strict fruiting management is required during the fruiting stage, with the temperature controlled at 18℃, humidity at 80%, light at 100 lux, and carbon dioxide at 3000ppM. Harvest after 28 days.

[0039] In this control example, the mycelium observation and the weight of the velvet antler mushroom products are as follows: Figure 3 shown.

[0040] Comparative Example 2

[0041] The mother culture medium for enhancing the vitality of Pleurotus eryngii strains described in this comparative example comprises the following raw materials in parts by weight: 200 g potato, 20 g glucose, 12 g agar powder, 5 ml kiwi juice, and 1300 ml water. The kiwi juice is obtained by squeezing fresh kiwi juice and filtering it.

[0042] Mix the raw materials of the mother culture medium and divide them into the culture medium. Sterilize the culture medium with high pressure steam at 121°C for 20 minutes and then inoculate the activated mother culture mycelium on the culture medium after cooling. Then, culture it at a constant temperature of 25°C in the dark for recovery. After the mycelium grows on the culture medium, draw a line on the culture medium to mark it and continue to culture for 20 days. During this period, draw a line every 4 days to observe and record the growth conditions of these 20 days, including growth potential, color, average growth rate, etc.

[0043] Weigh the raw materials required for preparing 1000 bags of conventional culture medium for cultivating velvet antler mushrooms according to the proportion, put them into the blender and mix them evenly, then add water and stir for 45 minutes until the water content reaches 65%, adjust the pH to 9.5, and bag them. The polypropylene bag used for bagging has a circumference of 38cm and a length of 36cm. The height after bagging is 19CM and the weight is 1500g.

[0044] After bagging, sterilize at 123℃ for 120min, and inoculate after cooling. After inoculation, culture in a dark room at 25℃ and 78% humidity for about 70 days. When the mycelium is white and strong, move it to the scratching room, remove the ring cover, and then move it to the fruiting room to enter the fruiting stage. Strict fruiting management is required during the fruiting stage, with the temperature controlled at 18℃, humidity at 80%, light at 100 lux, and carbon dioxide at 3000ppM. Harvest after 28 days.

[0045] In this control example, the mycelium observation and the weight of the velvet antler mushroom products are as follows: Figure 4 shown.

[0046] Comparative Example 3

[0047] The mother culture medium for enhancing the vitality of Pleurotus eryngii strains described in this comparative example comprises the following raw materials in parts by weight: 200 g potato, 20 g glucose, 12 g agar powder, 100 ml kiwi juice, and 1300 ml water. The kiwi juice is obtained by squeezing fresh kiwi juice and filtering it.

[0048] Mix the raw materials of the mother culture medium and divide them into the culture medium. Sterilize the culture medium with high pressure steam at 121°C for 20 minutes and then inoculate the activated mother culture mycelium on the culture medium after cooling. Then, culture it at a constant temperature of 25°C in the dark for recovery. After the mycelium grows on the culture medium, draw a line on the culture medium to mark it and continue to culture for 20 days. During this period, draw a line every 4 days to observe and record the growth conditions of these 20 days, including growth potential, color, average growth rate, etc.

[0049] Weigh the raw materials required for preparing 1000 bags of conventional culture medium for cultivating velvet antler mushrooms according to the proportion, put them into the blender and mix them evenly, then add water and stir for 45 minutes until the water content reaches 65%, adjust the pH to 9.5, and bag them. The polypropylene bag used for bagging has a circumference of 38cm and a length of 36cm. The height after bagging is 19CM and the weight is 1500g.

[0050] After bagging, sterilize at 123℃ for 120min, and inoculate after cooling. After inoculation, culture in a dark room at 25℃ and 78% humidity for about 70 days. When the mycelium is white and strong, move it to the scratching room, remove the ring cover, and then move it to the fruiting room to enter the fruiting stage. Strict fruiting management is required during the fruiting stage, with the temperature controlled at 18℃, humidity at 80%, light at 100 lux, and carbon dioxide at 3000ppM. Harvest after 28 days.

[0051] In this control example, the mycelium observation and the weight of the velvet antler mushroom products are as follows: Figure 5 shown.

[0052] Comparative Example 4

[0053] The mother culture medium for enhancing the vitality of Pleurotus eryngii strains described in this comparative example comprises the following raw materials in parts by weight: 200 g potato, 20 g glucose, 12 g agar powder, 1300 ml water, and also contains 0.1 mol of vitamin C.

[0054] Mix the raw materials of the mother culture medium and divide them into the culture medium. Sterilize the culture medium with high pressure steam at 121°C for 20 minutes and then inoculate the activated mother culture mycelium on the culture medium after cooling. Then, culture it at a constant temperature of 25°C in the dark for recovery. After the mycelium grows on the culture medium, draw a line on the culture medium to mark it and continue to culture for 20 days. During this period, draw a line every 4 days to observe and record the growth conditions of these 20 days, including growth potential, color, average growth rate, etc.

[0055] Weigh the raw materials required for preparing 1000 bags of conventional culture medium for cultivating velvet antler mushrooms according to the proportion, put them into the blender and mix them evenly, then add water and stir for 45 minutes until the water content reaches 65%, adjust the pH to 9.5, and bag them. The polypropylene bag used for bagging has a circumference of 38cm and a length of 36cm. The height after bagging is 19CM and the weight is 1500g.

[0056] After bagging, sterilize at 123℃ for 120min, and inoculate after cooling. After inoculation, culture in a dark room at 25℃ and 78% humidity for about 70 days. When the mycelium is white and strong, move it to the scratching room, remove the ring cover, and then move it to the fruiting room to enter the fruiting stage. Strict fruiting management is required during the fruiting stage, with the temperature controlled at 18℃, humidity at 80%, light at 100 lux, and carbon dioxide at 3000ppM. Harvest after 28 days.

[0057] In this control example, the mycelium observation and the weight of the velvet antler mushroom products are as follows: Figure 6 shown.

[0058] Comparative Example 5

[0059] The mother culture medium for enhancing the vitality of Pleurotus eryngii strains described in this comparative example includes the following raw materials in parts by weight: 200g potato, 20g glucose, 12g agar powder, 1300ml water, and also includes 3mol of vitamin C.

[0060] Mix the raw materials of the mother culture medium and divide them into the culture medium. Sterilize the culture medium with high pressure steam at 121°C for 20 minutes and then inoculate the activated mother culture mycelium on the culture medium after cooling. Then, culture it at a constant temperature of 25°C in the dark for recovery. After the mycelium grows on the culture medium, draw a line on the culture medium to mark it and continue to culture for 20 days. During this period, draw a line every 4 days to observe and record the growth conditions of these 20 days, including growth potential, color, average growth rate, etc.

[0061] Weigh the raw materials required for preparing 1000 bags of conventional culture medium for cultivating velvet antler mushrooms according to the proportion, put them into the blender and mix them evenly, then add water and stir for 45 minutes until the water content reaches 65%, adjust the pH to 9.5, and bag them. The polypropylene bag used for bagging has a circumference of 38cm and a length of 36cm. The height after bagging is 19CM and the weight is 1500g.

[0062] After bagging, sterilize at 123℃ for 120min, and inoculate after cooling. After inoculation, culture in a dark room at 25℃ and 78% humidity for about 70 days. When the mycelium is white and strong, move it to the scratching room, remove the ring cover, and then move it to the fruiting room to enter the fruiting stage. Strict fruiting management is required during the fruiting stage, with the temperature controlled at 18℃, humidity at 80%, light at 100 lux, and carbon dioxide at 3000ppM. Harvest after 28 days.

[0063] In this control example, the mycelium observation and the weight of the velvet antler mushroom products are as follows: Figure 7 shown.

[0064] The mycelium growth rate, mycelium color and mycelium growth potential of the mother strains of Pleurotus eryngii in each embodiment and the control example were statistically analyzed, and the cultivation cycle and yield of Pleurotus eryngii cultivated from the activated mother strains were further statistically analyzed. The results are shown in Table 1.

[0065] Table 1

[0066]

[0067]

[0068] As shown in Table 1, according to Example 1, Example 2 and Comparative Example 1, the use of the mother culture medium of the present invention can effectively improve the vitality of the mother strain, and after inoculation, it can effectively improve the cultivation period of Porcupine, reduce the cultivation cost, and improve the quality and yield of the finished product of Porcupine. According to Comparative Examples 2 to 5, in the present invention, when a small amount of kiwi juice or vitamin C is added, it can promote the growth of mother strain mycelium to a certain extent, and is conducive to shortening the cultivation period and increasing the yield after further cultivation of Porcupine, and when kiwi juice or vitamin C is added in excess, it will inhibit the growth of mycelium, and in the subsequent further cultivation of Porcupine, the cultivation period and product cannot be improved, indicating that the reasonable combination of each raw material component in the culture medium and kiwi juice or vitamin C is extremely critical.

Claims

1. A mother culture medium for enhancing the activity of Pleurotus eryngii strains, characterized in that: The mother seed culture medium consists of potatoes, glucose, agar powder, adjuvants and appropriate amount of water.

2. The mother culture medium for enhancing the activity of Pleurotus eryngii strains according to claim 1, characterized in that: The auxiliary agent is kiwi juice or vitamin C.

3. The mother culture medium for enhancing the activity of Pleurotus eryngii strains according to claim 2, characterized in that: The mother seed culture medium comprises the following raw materials in parts by weight: 150-250 parts of potato, 15-25 parts of glucose, 10-15 parts of agar powder, 45-55 parts of kiwi juice and 1200-1400 parts of water.

4. The mother culture medium for enhancing the activity of Pleurotus eryngii strains according to claim 3, characterized in that: The mother seed culture medium comprises the following raw materials in parts by weight: 200 parts of potatoes, 20 parts of glucose, 12 parts of agar powder, 50 parts of kiwi juice, and 1300 parts of water.

5. The mother culture medium for enhancing the activity of Pleurotus eryngii strains according to any one of claims 2 to 4, characterized in that: The kiwifruit juice is obtained by squeezing fresh kiwifruit and then filtering it.

6. The mother culture medium for enhancing the activity of Pleurotus eryngii strains according to claim 2, characterized in that: The mother seed culture medium comprises the following raw materials in parts by weight: 150-250g of potato, 15-25g of glucose, 10-15g of agar powder, and 1200-1400ml of water. The content of vitamin C in the culture medium is 0.4-0.6mol.

7. The mother culture medium for enhancing the activity of Pleurotus eryngii strains according to claim 6, characterized in that: The mother culture medium comprises the following raw materials in parts by weight: 200 g potato, 20 g glucose, 12 g agar powder, and 1300 g water. In addition, the content of vitamin C in the culture medium is 0.5 mol.

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