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Application of streptomyces aureus as marker for detecting and diagnosing hypertension to preparation of corresponding detection tool

A technology of Streptomyces aureus, detection tool, applied in the field of biomarkers

Pending Publication Date: 2021-06-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] After investigation, there are no domestic and foreign scholars’ related literature reports on the correlation between the microorganism Streptomyces aureus Tü117 and hypertension (from PUBMED, CNKI, VIP China Journal Network), so the intestinal bacterium Streptomyces aureus Tü117 plays an important role in the study of hypertension biomarkers. The field is still in the blank stage

Method used

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  • Application of streptomyces aureus as marker for detecting and diagnosing hypertension to preparation of corresponding detection tool
  • Application of streptomyces aureus as marker for detecting and diagnosing hypertension to preparation of corresponding detection tool
  • Application of streptomyces aureus as marker for detecting and diagnosing hypertension to preparation of corresponding detection tool

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Higher levels of Streptomyces aureus Tü117 in high-salt diet-induced hypertensive mice

[0051]Total fecal RNA was extracted from 7 high-salt diet-induced hypertensive C57BL / 6J mice and 6 normal-fed control C57BL / 6J mice, and the concentration and absorbance were measured; the obtained RNA was reverse-transcribed into cDNA; The obtained cDNA was amplified on a quantitative PCR instrument; the primers required for the amplification were provided by Streptomyces aureus Tü117-specific gene LipReg4 (Horbal L, Rebets Y, Rabyk M, Luzhetskyy A, Ostash B, Welle E, Nakamura T, Fedorenko V , Bechthold A.Characterization and analysis of the regulatory network involved in control of lipomycin biosynthesis in Streptomyces aureofaciens Tü117.Applied microbiology and biotechnology.2010; 85:1069-1079) forward primer sequence and reverse primer sequence composition, forward primer sequence It is: 5'-TGGATTTGCTCATCGAGAGG-3', and the reverse primer sequence is: 5'-TTCTGCCGTTTCC...

Embodiment 2

[0054] Example 2: The level of Streptomyces aureus Tü117 in the hypertensive population is higher

[0055] A total of 104 volunteers were recruited in this clinical trial, including 26 healthy subjects and 78 hypertensive subjects. All volunteers signed an informed consent form, and all volunteers met the inclusion and exclusion criteria. The standards are as follows:

[0056] Inclusion criteria: healthy people or clinically diagnosed hypertension.

[0057] Exclusion criteria: (1) known or suspected secondary hypertension; (2) suffering from severe chronic diseases (including heart failure, renal failure, lung disease, rheumatism, stroke, gastrointestinal disease or blood disease, etc.) ; (3) have received antibiotics or probiotics treatment in the past 1 month; (4) have severe cardiovascular and cerebrovascular diseases and related surgery history; (5) have gastrointestinal inflammation or take prescribed anti-inflammatory drugs or may affect Supplements / drugs for inflamma...

Embodiment 3

[0063] Example 3: Evaluation of the diagnostic effect of Streptomyces aureus Tü117 on hypertensive patients

[0064] According to the relative level of Streptomyces aureus Tü117 in fecal samples, the correlation between Tü117 and hypertensive patients was analyzed using single factor logistic regression. The results are shown in the table below, indicating that there is a strong correlation between Tü117 and hypertensive patients.

[0065] Table 4 Single factor logistic regression analysis results

[0066]

[0067] According to 2 -△△Ct The relative expression of Tü117 in feces was calculated, and SPSS software was used for regression analysis, and the formula was obtained: Logit(P)=4.077×LipReg4–5.787. Substitute the detected relative expression of LipReg4 into the above equation, if the calculated value is higher than 0.41, it is diagnosed as hypertension, and if the calculated value is lower than 0.41, it is judged as non-hypertensive.

[0068] In order to evaluate the ...

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Abstract

The invention discloses application of streptomyces aureus Tu117 as a marker for detecting and diagnosing hypertension to preparation of a corresponding detection tool. A detection object of the detection tool is excrement, intestinal tissues or intestinal contents collected from a subject. The streptomyces aureus Tu117 of the invention is relatively high in content in bodies of hypertension model mice and hypertension patients, can be used for establishing a detection method or preparing a detection and diagnosis kit, and is used for auxiliary diagnosis and risk assessment of hypertension and prehypertension.

Description

technical field [0001] The present invention relates to a biomarker, in particular to a Streptomyces aureus Tü117 biomarker in feces and its application. Background technique [0002] Cardiovascular diseases such as hypertension are the primary cause of harm to human health and death. The prevalence of hypertension is increasing year by year. The China Hypertension Survey (Status of hypertension in China: results from the China Hypertension Survey, 2012-2015. Circulation, 2018; 137(22): 2344-2356) used stratified, multi-stage, random sampling methods from 2012 to 2015 A survey of 451,755 residents aged ≥18 years was conducted in 262 cities and villages in 31 provinces in mainland China. The results showed that the prevalence of hypertension in Chinese adults was 27.9%, which was higher in men than in women (28.6% vs. 27.2%). The incidence rate increases with age. [0003] Although elevated blood pressure is the most important clinical manifestation of hypertension, it is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12Q1/689C12Q1/6851C12R1/49
CPCC12Q1/06C12Q1/689C12Q1/6851G01N2333/36G01N2800/321C12Q2531/113C12Q2521/107
Inventor 马鑫周婷婷翟齐啸王志玮郭梦婷刘靓靓肖望
Owner JIANGNAN UNIV
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