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Chlortetracycline fermentation production method using refined protein powder

A production method, the technology of chlortetracycline, which is applied in the field of chlortetracycline fermentation production, can solve the problems of high raw material cost and high price, and achieve the effect of reducing raw material cost, increasing output, and efficiently producing chlortetracycline

Inactive Publication Date: 2016-10-26
PUCHENG CHIA TAI BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Soybean cake powder is widely used in the fermentation industry, but compared with other organic nitrogen sources, its price is relatively high, resulting in high raw material costs

Method used

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  • Chlortetracycline fermentation production method using refined protein powder

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Embodiment 1

[0023] Such as figure 1 As shown, the present invention discloses a fermentation production method of chlortetracycline using refined protein powder instead of soybean meal powder. Streptomyces aureus is used as the starting strain to produce chlortetracycline through pure culture and secondary fermentation. The process steps include mother bottle Spore liquid preparation and preservation process, sub-bottle slope spore liquid preparation process, primary seed preparation process and fermentation preparation process:

[0024] The steps of the mother bottle spore liquid preparation and preservation process are as follows: under aseptic conditions, use the inoculating spatula to scrape off the bacterial layer of the bacterial seed slant and add appropriate amount of glycerin water or sterile water according to the storage requirements. After stirring evenly, filter with a funnel. The filtered bacterial liquid is the mother bottle spore liquid, which is divided into different specif...

Embodiment 2

[0033] Experimental purpose: In order to verify the influence of different medium ratios on the fermentation production of chlortetracycline, this example compares the 100% refined protein powder instead of soybean meal powder and 100% soybean meal powder for the chlortetracycline fermentation formula, and performs corresponding Chlortetracycline potency determination and cost accounting.

[0034] Experimental method: Using the culture method in Example 1, the chlortetracycline fermentation formula group that implements refined protein powder 100% instead of soybean cake powder is defined as the refined protein powder group, and 100% chlortetracycline fermentation of soybean cake powder is implemented The formula group is defined as the soybean meal group. Under the same conditions, change the ratio of medium per liter in the fermentation tank in the fermentation preparation process. The specific ratio is as follows:

[0035] Refined protein powder group: peanut meal powder 20g, re...

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Abstract

The invention relates to the field of fermentation production processes, and discloses a chlortetracycline fermentation production method using refined protein powder. According to the present invention, Streptomyces aureus is adopted as a starting strain, and pure culture and two-stage fermentation are performed to produce chlortetracycline; with the application of the 100% refined protein powder to replace the soybean cake powder, the chemical titer is increased by 2.3% compared to the application of the soybean cake powder, and the yield is increased by 1.8% compared to the unimproved method, such that the yield is effectively increased; and the raw material cost input per tank can be reduced by about 540 yuan after the cost accounting, the effect is good, the fermentation production level is stably increased, and the theoretical and applied basis is provided for the industrial production of the antibiotics and the scientific research.

Description

Technical field [0001] The invention relates to the field of fermentation production technology, in particular to a fermentation production method of chlortetracycline using refined protein powder. Background technique [0002] Chlortetracycline (CTC) is a broad-spectrum antibiotic belonging to the tetracycline class and has a wide range of uses in medicine, animal husbandry and agriculture. Especially in the animal husbandry industry, it is one of the eight antibiotic products (chlortetracycline, tylosin and oxytetracycline, etc.) used as feed drug additives. Chlortetracycline is used in the body for its antibacterial, growth-promoting and high feed utilization rate. The characteristics of low internal residues have become the most used antibacterial growth promoter in the feed industry at present and in the future. Chlortetracycline products in my country have been exported to developed countries such as Europe and the United States. In recent years, the research of tetracycl...

Claims

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Application Information

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IPC IPC(8): C12P29/00C12R1/49
CPCC12P29/00
Inventor 杨占英吴新芝陈浩然向玉华朱忠斌
Owner PUCHENG CHIA TAI BIOCHEM
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