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Biological pesticide producing process

A production method and technology of biological pesticides, applied in the fields of botanical equipment and methods, biocides, animal repellents, etc., can solve problems such as unsolvable and pollution, and achieve the effects of broad antibacterial spectrum, convenient use and significant control effect.

Inactive Publication Date: 2002-02-20
王玉兰
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AI Technical Summary

Problems solved by technology

Although my country has also taken some measures and banned the production of some highly toxic (such as organophosphorus, mercury, arsenic, etc.) pesticides, it still cannot solve the problem of pollution. Therefore, in recent years, my country has also vigorously advocated the use of biological pesticides to develop green Agriculture, so agriculture is eager to provide a non-toxic and harmless biological pesticide to solve the cropping disease, to replace the chemical pesticides still in use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] The production method steps are as follows:

[0027] 1. Activation and expanded culture of the original strain of Streptomyces aureus: inoculate the original bacteria on Gao’s No. 1 medium, and cultivate it at 28°C for 4-5 days. catch.

[0028] 2. Shake flask shaking culture: Inoculate the activated bacteria into the shaking flask, medium capacity: 300-1000 ml triangular flask, shake and culture at 28C for 48 hours, wait for the mycelium to grow and stretch without any bacteria, do For fermenting bacteria.

[0029] 3. Primary fermentation culture: Inoculate the seeds cultivated by shaking the flask into a 50-100 liter primary fermentation tank with a 5% inoculation amount, the ventilation rate is 1:0.5, and the tank is stirred at 220 rpm , use rich medium, pH is 7.-7.4, temperature is 28°C, ferment for 24-36 hours, when the mycelium is strong, stretched, and there are no bacteria, it can be transferred to the secondary fermentation.

[0030] 4. Secondary fermentation...

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Abstract

The manufacturing method of biopesticide is characterized as follows: (1) the streptomyces aureus bacterial spawn is first stage cultured in first stage fermentation tank with air volume 1:0.3-0.6 and stirring 200-250 rpm, temperature 27-30 deg.C for 24-36 hrs; (2) and stage cultured with air volume 1:0.3-0.6, stirring 180-210 rpm, temperature 27-30 deg.c for 28-32 hr; (3) third stage cultured with culture dedium pH 7.2-7.4, air volume 1:0.2-0.4, stirring 160 rpm for 48-50 hr.. The invented biopesticide is of high effective, no poison, no hazard etc.

Description

Technical field: [0001] The invention relates to a method for producing pesticides. Background technique: [0002] Crop diseases are one of the main factors causing crop yield reduction. Severe diseases can lead to a substantial reduction in yield, or even non-harvesting of grains. Therefore, agricultural production puts the prevention and control of diseases in a primary position. In order to solve the off-season problem of vegetables and fruits, especially in northern my country where the temperature is cold, many crops cannot be produced in winter. The situation is getting worse every year, and many crops cannot be replanted. Although the control of chemical pesticides has a certain effect, the pollution of pesticides is also quite serious. Due to the long-term application of chemical pesticides, many bacteria have developed drug resistance, which has led to an increase in the disease in recent years. Because the dosage of pesticides is getting larger and larger, many cr...

Claims

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Application Information

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IPC IPC(8): A01N63/00
Inventor 王玉兰
Owner 王玉兰
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