Preparation method and application of rare earth luminescent complex

A rare earth luminescence and complex technology, applied in the measurement of color/spectral properties, etc., to achieve the effects of improved detection and diagnosis probability, high sensitivity and strong practicability

Active Publication Date: 2019-05-21
陕西麦可罗生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there are few reports on the use of rare earth luminescent

Method used

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  • Preparation method and application of rare earth luminescent complex
  • Preparation method and application of rare earth luminescent complex
  • Preparation method and application of rare earth luminescent complex

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preparation example Construction

[0042] The preparation method of the rare earth luminescent complex of the present invention is as follows:

[0043] (1) Synthesis of thioether

[0044] Weigh 2.722g of sodium ethoxide solid and dissolve it in 100mL of absolute ethanol in a 250mL round-bottomed flask, add 4.968g of p-methylthiophenol and 8g of 2-bromoacetophenone in turn under mechanical stirring, and place it in a 65°C oil Stir under the bath for 1.5h, then add ice cubes to precipitate a milky yellow solid, filter it with suction, and dry it in a desiccator until constant weight. Add absolute ethanol to the crude product, put it in the refrigerator for recrystallization, and filter to obtain white needle-like crystals. Melting point mp: 38.5-39.5°C.

[0045]

[0046] (2) Synthesis of sulfoxide

[0047] At room temperature, dissolve 1.5 g of thioether in 50 mL of glacial acetic acid solution, add 1 mL of 30% H 2 o 2 ,, stirred for 12 hours, poured the obtained solution into water, extracted with ether,...

Embodiment 1

[0059] (1) Weigh 2.722g of sodium ethoxide solid and dissolve it in 100mL of absolute ethanol in a 250mL round bottom flask, add 4.968g of p-methylthiophenol and 8g of 2-bromoacetophenone in sequence under mechanical stirring, and place in Stir in an oil bath at 65°C for 1.5 hours, then add ice cubes to precipitate a milky yellow solid, filter it with suction, and dry it in a desiccator until constant weight. Add absolute ethanol to the crude product, put it in the refrigerator for recrystallization, and filter to obtain white needle-like crystals. Melting point mp: 38.5-39.5°C.

[0060] (2) At room temperature, dissolve 1.5g of thioether in 50mL of glacial acetic acid solution, add 1mL of 30% H 2 o 2 ,, stirred for 12 hours, poured the obtained solution into water, extracted with ether, and added water to wash repeatedly until the organic phase was neutral, and a large amount of white flocculent solids were precipitated at the same time, filtered by suction to obtain a whit...

Embodiment 2

[0068] The zero-grade tank of Streptomyces aureus, after the zero-grade tank of 5L is sterilized by high-temperature steam, the spore suspension of Streptomyces aureus on the inclined plane of a large test tube resurrected from the Streptomyces aureus strain storage room is inoculated with the following medium: soybean meal powder 5.0-8.0%; NaCl: 0.3-0.5%; corn steep liquor dry powder: 0.5-1.0%; maltose: 2.0-2.5%; fish oil: 3.5-4.0%; KH2PO4: 0.03-0.05%; ; The pH value is 6.8-7.2; the fermentation conditions are ventilation rate 1:1, stirring speed 150-200rpm, tank pressure 0.02MPa. Fermentation time 0~19h, take 100mL fermentation sample of fermented liquid from the zero-grade tank, ultracentrifuge the sample at 12000rpm, centrifugation time is 30min, take 100mL of supernatant each, and then add a certain amount of powder (particle size 200nm) Eu(pps-Si) 2 ·(ClO 4 ) 3 ·6H 2 O, after magnetic stirring for 30min, then nanofiltration to remove water, with a concentration of 10...

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Abstract

The invention relates to a preparation method and application of a rare earth luminescent complex. Through synthesis of (2, 2-triethoxysilylpropionamido) benzoyl-p-tolylsulfoxide, with (2, 2-triethoxysilylpropionamido) benzoyl-p-tolylsulfoxide as a ligand, an europium rare earth perchlorate solid binary complex Eu (pps-Si)2.(ClO4)3.6H2O is synthesized, the solid binary complex can be well used asa fluorescence indicator to detect whether mixed fungi pollution happens during a Kasugamycin production process by fermentation of Streptomyces microaureus, and the indicator has high sensitivity, simple operation and strong practicability. When the method is used for detecting mixed fungi during the Kasugamycin production process by fermentation of Streptomyces microaureus, the detection procedure is optimized, and the method has the characteristics of simple operation, strong practicability and the like.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a preparation method and application of a rare earth luminescent complex, and more specifically to the application of the rare earth luminescent complex to the detection of Streptomyces aureus infection in the fermentation process. Background technique [0002] One of the most difficult things encountered in microbial fermentation is how to control the probability of microbial contamination during the fermentation process. There are many factors involved in controlling the probability of bacterial contamination, such as the production process of bacteria supply and various physical parameters in the batch fermentation process, such as vapor pressure, Tank pressure, rotation speed, foaming, oxygen utilization rate (OUR, for aerobic fermentation), disinfection and aseptic inoculation during fermentation, etc. During microbial fermentation, staining and microscopic exa...

Claims

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Application Information

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IPC IPC(8): G01N21/33
Inventor 潘忠成袁士瑞李昶志李蒲民
Owner 陕西麦可罗生物科技有限公司
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