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Fermenting method for increasing pleocidin output

A fermentation method and technology of spinosad, which is applied in the field of fermentation to increase the yield of spinosad, can solve the problems of complex fermentation process, low fermentation yield, and high production cost, and achieve the effects of increasing fermentation unit, increasing yield, and being easy to absorb

Active Publication Date: 2017-12-29
SHANGHAI NONGLE BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main problems restricting the industrialization and wide application of spinosad are high production cost, complicated fermentation process and low fermentation yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Saccharopolyspora spinosa (Saccharopolyspora spinosa) SP5266 was used to ferment and produce spinosyn. The fermentation medium used was: glucose 45g / L, soybean meal 10g / L, yeast extract 14g / L, corn steep liquor 8g / L, soybean oil 20g / L L, ph7.0

[0017] Using the above medium, ferment in a 50-ton fermenter according to the following conditions: culture temperature 28°C, rotation speed 210rpm, aeration ratio 1:1, tank pressure 0.03MPa, dissolved oxygen controlled above 60%, and a fermentation period of 216hr. At 96 hours after the start of fermentation, the compound organic nitrogen source was added for the first time to a final concentration of 12g / L, and the weight ratio of peanut meal and cottonseed meal was 5:4. At 144 hours after the start of fermentation, the compound organic nitrogen source was added for the second time to a final concentration of 5g / L, and the weight ratio of peanut cake powder and cottonseed cake powder was 5:4.

[0018] After 216 hours of ferme...

Embodiment 2

[0020] Saccharopolyspora spinosa (Saccharopolyspora spinosa) SP5266 was used to ferment and produce spinosyn. The fermentation medium used was: glucose 45g / L, soybean meal 10g / L, yeast extract 14g / L, corn steep liquor 8g / L, soybean oil 20g / L L. ph7.0

[0021] Using the above medium, ferment in a 50-ton fermenter according to the following conditions: culture temperature 28°C, rotation speed 210rpm, aeration ratio 1:1, tank pressure 0.03MPa, dissolved oxygen controlled above 60%, and a fermentation period of 216hr. At 96 hours after the start of fermentation, the compound organic nitrogen source was added for the first time to a final concentration of 20 g / L, and the weight ratio of peanut cake powder and cottonseed cake powder was 3:7. At 144 hours after the start of fermentation, the compound organic nitrogen source was added for the second time to a final concentration of 20 g / L, and the weight ratio of peanut cake powder and cottonseed cake powder was 3:7.

[0022] After ...

Embodiment 3

[0024] Saccharopolyspora spinosa (Saccharopolyspora spinosa) SP5266 was used to ferment and produce spinosyn. The fermentation medium used was: glucose 45g / L, soybean meal 10g / L, yeast extract 14g / L, corn steep liquor 8g / L, soybean oil 20g / L L. ph7.0

[0025] Using the above medium, ferment in a 50-ton fermenter according to the following conditions: culture temperature 28°C, rotation speed 210rpm, aeration ratio 1:1, tank pressure 0.03MPa, dissolved oxygen controlled above 60%, and a fermentation period of 216hr. At 96 hours after the start of fermentation, the compound organic nitrogen source was added for the first time to a final concentration of 5g / L, and the weight ratio of peanut meal and cottonseed meal was 1:1. At 144 hours after the start of fermentation, the compound organic nitrogen source was added for the second time to a final concentration of 5g / L, and the weight ratio of peanut meal and cottonseed meal was 1:1.

[0026] After 216 hours of fermentation, the s...

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PUM

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Abstract

The invention relates to a fermenting method for increasing pleocidin output. The method comprises the following steps: putting saccharopolyspora spinosa in a fermenting culture medium to ferment; after fermentation in 72-120hr, supplementing a compound organic nitrogen source for the first time, wherein the adding amount is the amount, by which the final concentration of the compound organic nitrogen source in a fermenting liquid is 5-20g / L; and after fermentation in 120-160hr, supplementing the compound organic nitrogen source for the first time, wherein the adding amount is the amount, by which the final concentration of the compound organic nitrogen source in the fermenting liquid is 5-20g / L. Continuous production of thalli and product synthesis are facilitated, so that the pleocidin output can be further increased.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a fermentation method for improving the yield of spinosyns. Background technique [0002] Spinosad is a macrolide antibiotic produced by the actinomycete Saccharopolyspora spinosa, and its most active components are spinosad A and spinosad D. Spinosad can effectively control most of Lepidoptera, Orthoptera, Diptera and other pests, and has low toxicity to mammals, birds and most beneficial insects. It has the advantages of broad insecticidal spectrum, high activity, harmless to non-target animals and safe decomposition products. [0003] At present, the main problems restricting the industrialization and wide application of spinosad are high production cost, complicated fermentation process and low fermentation yield. Optimizing the culture conditions, thereby improving the fermentation titer and reducing the production cost is a research focus to promote the industrializa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/62C12R1/01
CPCC12P19/62
Inventor 施扬玮张进武沈丽娟张红艳龚新进
Owner SHANGHAI NONGLE BIOLOGICAL PROD
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