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Actinoplanes strain and application thereof

一种游动放线菌、非达霉素的技术，应用在微生物工程领域，能够解决生产成本高、效价低、产量波动性大等问题，达到产量提高、提高发酵单位、实现工业化生产的效果

Active Publication Date: 2015-04-29
ZHEJIANG HISUN PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above Tiacumicin and Lipiarmycin producing bacteria have the disadvantages of low titer of the target product (Tiacumicin and Lipiarmycin), large yield fluctuation, instability, high production cost, etc. Therefore, a new high-efficiency Fidaxomicin (Fidaxomicin) production strain is sought work still in progress

Method used

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  • Actinoplanes strain and application thereof
  • Actinoplanes strain and application thereof
  • Actinoplanes strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: strain source

[0031] Actinoplanes sp.HS-16-20 (Actinoplanes sp.HS-16-20, CGMCC NO.7294) is an actinomycetes isolated from the soil samples of Baiyun Mountain in Taizhou, Zhejiang.

[0032] Cross-sampling was carried out in the area designated by Baiyun Mountain in Taizhou, 5 sampling points were randomly selected, 10g of soil samples were taken from each point, put into a conical flask, mixed evenly, 10g of samples were taken, and added to a 90mL deionized In the Erlenmeyer flask of water (there is a magnetic stirrer in the bottle), vortex stirs 30min, makes it fully mix to make suspension liquid, is 10 -1 bacteria liquid. Dilute the above suspension with sterile water at a volume ratio of 1:9 to 10 by dilution coating plate method. -2 , 10 -3 , 10 -4 , 10 -5 Concentration, take 0.1mL of bacterial solution with different dilution multiples, spread it on the IPS2 medium plate, gently spread it on the surface of the medium with a sterile coating stick...

Embodiment 2

[0033] Embodiment 2: Morphological and cultural characteristics of actinomycete HS-16-20 (Actinoplanes sp.HS-16-20, CGMCC NO.7294)

[0034]The experiments were carried out with reference to the relevant content in the "Common Bacterial System Identification Manual", "Molecular Cloning Experiment Guide" and "Chinese Pharmacopoeia" (2010 edition). The morphological and cultural characteristics of the strains were tested using 10 culture media, including ISP1, ISP2, ISP3, ISP4, ISP5, Gaoshi No. 1, calcium malate, LB, YMS and Chase medium, and cultured at 28°C for 7 to 10 days. Observe the color and pigmentation of the mycelium. The results are shown in Table 1.

[0035] Table 1 Culture characteristics of bacterial strain HS-16-20 on 10 kinds of media

[0036]

[0037]

Embodiment 3

[0038] Embodiment 3: Physiological and biochemical characteristics of Actinomycetes HS-16-20 (Actinoplanes sp.HS-16-20, CGMCC NO.7294)

[0039] Utilization of carbon sources: ISP9 was used as the basal medium, and the final concentration of various carbon sources was 1.0%. The results are shown in Table 2.

[0040] Utilization of inorganic nitrogen sources: ISP9 was used as the basic medium, and the concentrations of potassium nitrate and ammonium sulfate were both 0.1%. The results are shown in Table 2.

[0041] The degradation test and the NaCl tolerance test used GYEA (pH6.8) as the base medium, and the results of the degradation test are shown in Table 3; the results of the NaCl tolerance test were as follows: NaCl tolerance was good, and growth was still possible in the presence of 10% NaCl.

[0042] Oxidase and catalase test, pH test and temperature test all use YMS medium. The results are: the strain can grow between 14°C and 45°C, and the optimum growth temperature ...

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Abstract

A bacterial strain of Actinoplanes sp. and an application thereof. The bacterial strain is named Actinoplanes sp. HS-16-20, whose preservation number is CGMCC No.7294. The bacterial strain can generate Fidaxomicin, and Fidaxomicin has an inhibitory effect on various gram positive bacteria pathogens, and in particular, on Clostridium difficile.

Description

technical field [0001] The invention relates to the technical field of microbial engineering, in particular to a strain of actinomycetes and its application in the preparation of Fidaxomicin. Background technique [0002] Diarrhea and colitis and other enteric diseases caused by Clostridium difficile infection are highly virulent and fatal. As early as 2003, Clostridium difficile caused outbreaks of infection in several Canadian provinces. As of October 2008, Clostridium difficile ribosomal type 027, which is highly pathogenic and fatal, has caused nosocomial infection outbreaks in at least 40 states of the United States. Shortly after Type 027 was discovered in North America, 17 European countries also experienced outbreaks of Clostridium difficile. Clostridium difficile has been listed as a mandatory surveillance pathogen in Europe, and three European epidemiological surveys were conducted in 2002, 2005, and 2008 under the leadership of the European Center for Disease Pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/62C12R1/045
CPCC12P19/62C12N1/205C12R2001/045A61P31/04C12N1/20
Inventor 徐浩汤飞詹佩君胡建红程莎莎任会军樊红梅白骅
Owner ZHEJIANG HISUN PHARMA CO LTD
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