White rot fungi, breeding method and use thereof

A technology of white rot fungi and strains, applied in fungi, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of slow development, low enzyme production level, and poor economic benefits.

Inactive Publication Date: 2009-07-01
HENAN YANGSHAO BIOCHEM ENG
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Second, judging from the current development of the domestic enzyme preparation industry, although laccase research started early, it developed slowly
[0004] At present, most domestic laccase research is still in the laboratory stage of research and development, and has

Method used

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  • White rot fungi, breeding method and use thereof
  • White rot fungi, breeding method and use thereof
  • White rot fungi, breeding method and use thereof

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Embodiment Construction

[0035] The white rot fungus (Trametes Versicolor) YS-L613 strain of the present invention is obtained by mutagenesis and breeding by the following method, using white rot fungi as the starting strain, using UV, 60 A compound mutagenesis breeding technique combining CO, ultraviolet light, nitrosoguanidine, ion implantation and microwave treatment was used to breed a high-yielding laccase strain YS-L613.

[0036] The required materials and preparation methods are as follows:

[0037] 1.1 Starting strain

[0038] White rot fungus YS strain, screened and preliminarily identified laccase-producing strains in our laboratory, stored on CPDA slope at 4°C.

[0039] 1.2 Medium

[0040] 1.2.1 Incline medium (CPDA): 20g glucose, 1.5g MgSO4·7H2O, 3g KH2PO4, 2mg VB1, 15g agar powder, 1000mL 20% potato juice.

[0041] 1.2.2 Shake flask liquid fermentation medium: glucose 20g, asparagine 2.5g, MgSO4 7H2O 0.5g, FeSO4 7H2O 10mg, MnSO4 4H2O 1mg, ZnSO4 7H2O 1mg, CuSO4 5H2O 2mg, NaHPO4 H2O 100m...

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Abstract

The invention relates to Trametes Versicolor YS-L613 and a breeding method and application thereof. The Trametes Versicolor YS-L613 is prepared by the method through mutation breeding. The breeding method is to take Trametes Versicolor as a starting strain, adopt composite mutation breeding technology which combines UV, 60 CO, ultraviolet rays, nitrosoguanidine, ion implantation and microwave treatment, and breed the YS-L613 strain for high-yield production of laccase. The invention performs breeding operation through physiochemical treatment methods such as ion implantation, the ultraviolet rays and mutagen, finally obtains the YS-L613 strain for high-yield production of the laccase, and adopts experimental automatic control technology, strain fermentation control technology and strain extraction technology to develop laccase products. As proved by experiments of the laccase in pulp bleaching, fiber modification and pulp deinking, utilization of the laccase in the paper-making industry can improve the quality of paper, reduce environmental pollution and save the cost.

Description

technical field [0001] The invention relates to a white-rot fungus (Trametes Versicolor) YS-L613, and also relates to a method for mutagenesis and breeding of the strain, in particular to a method for producing laccase by using the strain. Background technique [0002] Laccase is an oxidoreductase, its function is mainly to catalyze redox reaction, so laccase can directly reduce molecular oxygen to water, and can catalyze a large amount of phenols in the absence of hydrogen peroxide and other secondary metabolites and oxidation of aromatic amines. Some laccases can efficiently oxidize ascorbic acid, and some fungal laccases can also catalyze the demethylation of lignin and methoxyphenolic acid. Laccase acts on a wide range of substrates, including polyphenols, methyl-substituted monophenols, aromatic amines, benzenethiols, polymethoxybenzenes, and other easily oxidized compounds, as well as many compounds that are structurally similar to p-diphenols , such as aminophenol, ...

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Application Information

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IPC IPC(8): C12N1/14C12N15/01C12N13/00C12N9/02C12R1/645
Inventor 常东民李市场吴慧霞茹群朵左雪梅王耀民王娜郭至瑞
Owner HENAN YANGSHAO BIOCHEM ENG
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