White rot fungi, breeding method and use thereof

Abstract

The invention relates to Trametes Versicolor YS-L613 and a breeding method and application thereof. The Trametes Versicolor YS-L613 is prepared by the method through mutation breeding. The breeding method is to take Trametes Versicolor as a starting strain, adopt composite mutation breeding technology which combines UV, 60 CO, ultraviolet rays, nitrosoguanidine, ion implantation and microwave treatment, and breed the YS-L613 strain for high-yield production of laccase. The invention performs breeding operation through physiochemical treatment methods such as ion implantation, the ultraviolet rays and mutagen, finally obtains the YS-L613 strain for high-yield production of the laccase, and adopts experimental automatic control technology, strain fermentation control technology and strain extraction technology to develop laccase products. As proved by experiments of the laccase in pulp bleaching, fiber modification and pulp deinking, utilization of the laccase in the paper-making industry can improve the quality of paper, reduce environmental pollution and save the cost.

Description

technical field [0001] The invention relates to a white-rot fungus (Trametes Versicolor) YS-L613, and also relates to a method for mutagenesis and breeding of the strain, in particular to a method for producing laccase by using the strain. Background technique [0002] Laccase is an oxidoreductase, its function is mainly to catalyze redox reaction, so laccase can directly reduce molecular oxygen to water, and can catalyze a large amount of phenols in the absence of hydrogen peroxide and other secondary metabolites and oxidation of aromatic amines. Some laccases can efficiently oxidize ascorbic acid, and some fungal laccases can also catalyze the demethylation of lignin and methoxyphenolic acid. Laccase acts on a wide range of substrates, including polyphenols, methyl-substituted monophenols, aromatic amines, benzenethiols, polymethoxybenzenes, and other easily oxidized compounds, as well as many compounds that are structurally similar to p-diphenols , such as aminophenol, ...

AI Technical Summary

Problems solved by technology

Second, judging from the current development of the domestic enzyme preparation industry, although laccase research started early, it developed slowly

[0004] At present, most domestic laccase research is still in the laboratory stage of research and development, and has not entered industrial scale production. The fundamental reasons are: first, the enzyme production level of the bacteria is low, the economic benefit is poor, and industrial production cannot be carried out; The process of large-scale production of strains is immature

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