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Method for industrially producing L-hydroxyproline through fermentation method

A hydroxyproline and fermentation technology, which is applied in the field of fermentation engineering, can solve the problems of L-hydroxyproline fermentation unit quality, cost and three wastes discharge, and achieve the effects of reducing production costs, increasing fermentation units, and avoiding three wastes pollution

Inactive Publication Date: 2016-04-20
TIANJIN JINGYE FINE CHEM
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Problems solved by technology

[0004] At present, the research on the production of L-hydroxyproline by microbial fermentation is still in the laboratory stage, and a mature industrial production process has not yet been formed. However, the advanced industrial production process, especially the solid culture and liquid fermentation culture methods of strains, fermentation broth The extraction method and the purification method of the concentrate have a vital impact on the fermentation unit, yield, quality, cost and waste discharge of L-hydroxyproline

Method used

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Examples

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Effect test

example 1

[0015] 1. Solid culture of strains: Use 2N NaOH solution to adjust the pH of the solid culture medium to 7.0, divide it into 10ml inclined test tubes, wrap it with kraft paper with a stopper, sterilize at 121°C for 15 minutes, take it out and cool it to 50°C in a sterile room Add 5ul of 10% AMP solution to the test tube, shake it well, place it at an angle of 30°, and place it in a sterile room after solidification; use an inoculation loop on the ultra-clean workbench to make a ring and spread the bacteria evenly on the inclined surface of the test tube, and place it at 37±1.0 Cultivate in an incubator at ℃ for 24 hours, and plant the mother bottle directly after the bacterial lawn grows; the strain is from the Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, and the strain is a strain of EscherichiaColiK12W3110; the solid medium of the strain is composed of yeast powder, peptone, Sodium chloride, agar, AMP composition, its formula is yeast powder 5g / ...

example 2

[0020] 1. Solid culture of bacteria: adjust the pH of the culture medium to 7.0 with 2N NaOH solution, pack it into 50ml eggplant bottles, wrap it in kraft paper with a stopper, sterilize at 121°C for 15 minutes, take it out and cool it to 55°C in a sterile room Add 25ul of 10% AMP solution to the eggplant bottle, shake it well, place it at an angle of 30°, and place it in a sterile room after solidification; use an inoculation loop to make a ring on the ultra-clean workbench and spread the bacteria evenly on the blank slope of the eggplant bottle, and place it for 37 Cultivate in an incubator at ±1.0°C for 38 hours. After the bacterial lawn grows, place it in a refrigerator at 0-5°C for later use; the strain is from the Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, and the strain is a strain of EscherichiaColiK12W3110; the solid medium of the strain It is composed of yeast powder, peptone, sodium chloride, agar and AMP, and its formula is yeast po...

example 3

[0025]1. Solid culture of bacteria: adjust the pH of the culture medium to 7.0 with 2N NaOH solution, pack it into 50ml eggplant bottles, wrap it in kraft paper with a stopper, sterilize at 121°C for 15 minutes, take it out and cool it to 60°C in a sterile room Add 25ul of 10% AMP solution to the eggplant bottle, shake it well, place it at an angle of 30°, and place it in a sterile room after solidification; use an inoculation loop to make a ring on the ultra-clean workbench and spread the bacteria evenly on the blank slope of the eggplant bottle, and place it for 37 Cultivate in an incubator at ±1.0°C for 36 hours. After the bacterial lawn grows, place it in a refrigerator at 0-5°C for later use; the strain is from the Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, and the strain is a strain of EscherichiaColiK12W3110; the solid medium of the strain It is composed of yeast powder, peptone, sodium chloride, agar and AMP, and its formula is yeast pow...

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Abstract

Provided is a method for industrially producing L-hydroxyproline through a fermentation method. The method comprises the steps of strain solid culture, fermentation culture, fermentation liquor extraction and purification. According to the method for industrially producing the L-hydroxyproline through the fermentation method, the L-hydroxyproline is industrially produced through a direct glucose fermentation method, the fermentation unit, yield and quality of the L-hydroxyproline is improved, the production cost is reduced, and three-waste pollution is avoided.

Description

technical field [0001] The invention relates to fermentation engineering, in particular to a method for industrialized production of L-hydroxyproline by microorganisms directly utilizing glucose fermentation. Background technique [0002] Hydroxyproline (Hyp) is an imino acid, which is the product of L-proline hydroxylation, and its molecular formula is C 5 h 9 NO 3 . Hydroxyproline is white flaky crystal or crystalline powder, slightly sweet, with a melting point of 274°C, easily soluble in water and slightly soluble in ethanol. Trans-4-hydroxyproline is more common in nature, and trans-4-hydroxyproline was first found in animal collagen. Trans-4-hydroxyproline is widely used in medicine, chemical industry, animal feed, nutrition and beauty industry, etc. [0003] At present, the methods for producing hydroxyproline reported at home and abroad mainly include proteolytic extraction, chemical synthesis, microbial fermentation and enzymatic conversion. Among them, proteol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/24C07D207/16C12R1/19
CPCC12P13/24C07D207/16
Inventor 赵体金林俊辉彭久合
Owner TIANJIN JINGYE FINE CHEM
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