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Lactobacillus plantarum PC28152 with broad-spectrum fungal inhibition activity and application of lactobacillus plantarum PC28152

A technology of PC28152 and Lactobacillus plantarum, which is applied to Lactobacillus plantarum PC28152 and its application fields, and can solve problems that endanger human health and food safety

Active Publication Date: 2021-01-08
完美(广东)日用品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, fungi such as Aspergillus flavus and Fusarium secrete a variety of mycotoxins in the process of reproduction, which seriously endanger human health and cause many food safety problems.

Method used

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  • Lactobacillus plantarum PC28152 with broad-spectrum fungal inhibition activity and application of lactobacillus plantarum PC28152
  • Lactobacillus plantarum PC28152 with broad-spectrum fungal inhibition activity and application of lactobacillus plantarum PC28152
  • Lactobacillus plantarum PC28152 with broad-spectrum fungal inhibition activity and application of lactobacillus plantarum PC28152

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preparation example Construction

[0029] In the present invention, the preparation method of the fermentation liquid preferably includes the following steps: inoculating Lactobacillus plantarum PC28152 in the MRS liquid medium, and culturing at 35-40°C for 10-14 hours to obtain the fermentation liquid. Further preferably, it also includes centrifuging the fermentation broth to obtain a supernatant. More preferably, the supernatant is filtered through a filter membrane to obtain a sterile fermentation broth. The MRS liquid medium formula of the present invention is: soybean peptone 10g, beef extract 5g, yeast powder 4g, glucose 20g, Tween-801mL, disodium hydrogen phosphate 2g, anhydrous sodium acetate 5g, triamine citrate 2g, sulfuric acid Manganese 0.02g, magnesium sulfate 0.1g, distilled water to volume 1L, adjust the initial pH to 6.20, and sterilize at 121°C for 15min; the filter membrane is preferably a 0.22μm filter membrane. The present invention does not have special limitation to the source of soybean...

Embodiment 1

[0034] Example 1 Isolation, identification and screening of Lactobacillus plantarum PC28152

[0035] 1. Isolation of strains

[0036] The Sichuan traditional kimchi samples were serially diluted with normal saline, and 200 μL of 10 -4 、10 -5 and 10 -6 The diluted solution was spread on the MRS agar medium containing cycloheximide (0.1wt%) and colistin sulfate (0.1wt%), and three parallels were made for each gradient. Put the coated plate in an incubator at 37°C for anaerobic culture for 24 to 72 hours, pick a single colony, streak and purify twice on the same medium as above, and then carry out Gram staining and catalase test. Gram-positive, hydrogen peroxide-negative bacteria are tentatively designated as plant lactic acid bacteria.

[0037] 2. Screening of bacterial strains

[0038] Double-layer plate spotting method: Pour the MRS agar medium into a petri dish as the lower plate, and spot 5 μL of Lactobacillus plantarum solution after it solidifies, cultivate at 37°C fo...

Embodiment 2

[0051] The preparation of embodiment 2 lactobacillus plantarum PC28152 aseptic fermentation supernatant and the mensuration of antibacterial activity

[0052] 1. Preparation of aseptic fermentation supernatant of Lactobacillus plantarum PC28152

[0053] Plant Lactobacillus PC28152 by 5 × 10 6 CFU / mL was inoculated in MRS liquid medium, cultured at 37°C for 12 hours, centrifuged at 4000×g for 10 minutes to collect the fermentation supernatant, and filtered through a 0.22 μm filter membrane to obtain a sterile fermentation supernatant.

[0054] 2. Determination of antibacterial activity of aseptic fermentation supernatant of Lactobacillus plantarum PC28152

[0055] According to the ratio of 10% (v / v), the aseptic fermentation supernatant of Lactobacillus plantarum PC28152 and the sterilized PDA medium were fully mixed and poured into a plate. After cooling and solidifying, spot 5 μL of fungal (Aspergillus flavus CICC2219, Penicillium chrysogenum BNCC185782, Penicillium expanse...

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Abstract

The invention provides a lactobacillus plantarum PC28152 with broad-spectrum fungal inhibition activity. The strain is preserved in China General Microbiological Culture Collection Center on July 6, 2020, and the preservation number is CGMCC No.20198. The lactobacillus plantarum PC28152 and the fermentation liquor thereof have the effect of inhibiting fungal activity in a broad spectrum, and particularly have an obvious inhibiting effect on aspergillus flavus, penicillium chrysogenum, penicillium expansum, fusarium moniliforme and cladosporium cladosporioides. When the strain is applied to food preservation and preservation, infection of spoilage fungi to food can be effectively inhibited, and the shelf life of the food is remarkably prolonged.

Description

technical field [0001] The invention belongs to the field of microbiology and biotechnology, in particular to a strain of Lactobacillus plantarum PC28152 with broad-spectrum fungal inhibitory activity and its application. Background technique [0002] Fungal contamination is one of the most important factors that cause quality changes in food raw materials and products during processing, transportation and storage. Fungi mainly exist in the form of mycelium, spores, and sclerotia in food. On the one hand, they will reduce the quality and nutritional value of food by depleting nutrients in food through their own growth and reproduction. On the other hand, fungi such as Aspergillus flavus and Fusarium secrete a variety of mycotoxins in the process of reproduction, which seriously endanger human health and cause many food safety problems. [0003] The prevention and control of fungal contamination in the food industry is mainly based on physical and chemical antiseptic methods...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L3/3571C12R1/25
CPCA23L3/3571A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/10
Inventor 张文羿扎木苏李康宁孟和毕力格
Owner 完美(广东)日用品有限公司
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