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Method for improving protective enzyme activities of salt-stressed turfgrass by adopting enhanced salt-tolerant microbial agent

A technology for microbial inoculants, composite microbial inoculants, applied in the directions of microorganism-based methods, biochemical equipment and methods, chemicals for biological control, etc.

Inactive Publication Date: 2015-06-24
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the research on the enhancement of microorganisms in the natural environment (or compost) in some specific directions to obtain some highly effective enhanced microbial agents

Method used

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  • Method for improving protective enzyme activities of salt-stressed turfgrass by adopting enhanced salt-tolerant microbial agent
  • Method for improving protective enzyme activities of salt-stressed turfgrass by adopting enhanced salt-tolerant microbial agent
  • Method for improving protective enzyme activities of salt-stressed turfgrass by adopting enhanced salt-tolerant microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] The preparation method of strengthening the salt-tolerant microbial bacterial agent:

[0092] （1） Prepare the concentration of salt (w / w): prepare the concentration of NaCl respectively: 0.5%, 1%, 1.5%, 2%, 2.5% and 3% for later use;

[0093] (2) During the domestication process, depending on the OD 600 Gradually increase the salt concentration, the concentration of NaCl is increased to 30g / L with a gradient of 5g / L, every time the concentration of NaCl is increased, after the growth of the bacteria is stable, the concentration of NaCl will continue to be increased, and the salt-tolerant mixture will be acclimated step by step. Microflora; Bacillus subtilis, Bacillus lysinus and Penicillium chrysogenum;

[0094] (3) Enhanced culture of salt-tolerant strains: expand the screened enhanced salt-tolerant microorganisms on the corresponding liquid medium, culture Bacillus subtilis and Bacillus lysine at 30°C, 180r / min for 1 day, and produce yellow and green Mold was cultu...

Embodiment 2

[0097] The method for improving the activity of salt-stressed turfgrass protective enzymes by strengthening the salt-tolerant microbial bacterial agent:

[0098] (1) Experimental materials: Lawn grasses were selected to select tall fescue with full grains and uniformity ( Festuca arundinacea L.) Seeds are the test materials;

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Abstract

The invention discloses a method for improving protective enzyme activities of salt-stressed turfgrass by adopting an enhanced salt-tolerant microbial agent. The enhanced salt-tolerant microbial agent comprises enhanced bacillus subtilis, enhanced lysine bacillus and enhanced penicillium chrysogenum in a weight part ratio of 1 to 1 to 1, wherein enhanced bacillus subtilis, enhanced lysine bacillus and enhanced penicillium chrysogenum are salt-tolerant mixed microbial flora obtained by carrying out domestication step by step with a method for increasing the salt concentration step by step. The invention further discloses an application of the enhanced salt-tolerant microbial agent in improvement of the protective enzyme activities of salt-stressed turfgrass festuca arundinacea L. Experimental results show that the compound enhanced microbial agent can conduce to effectively alleviating the harms of saline-alkali soil to the turfgrass; screening and culturing corresponding enhanced salt-tolerant compost microbial strains and carrying out artificial inoculation is a new way for improving the salt tolerance of the turfgrass.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to a method for improving the activity of salt-stressed turfgrass protective enzymes by using a strengthened salt-tolerant microbial bacterial agent. Background technique [0002] Microbial agents, especially compound microbial agents composed of a variety of microorganisms, are biotechnology developed in recent years with broad application prospects. Microbial agents can not only repair oil-contaminated soil and degrade organic pollutants in water bodies, but also prevent and control pests and diseases, promote crop production and increase production and income. Some microbial agents can improve the stress resistance of plants. Since the 1970s, European and American Japan and other countries or regions have successfully developed some composite bacterial agents, many of which have begun large-scale production and formed serialized products. Among them, EM (Effective...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01N63/04A01P21/00A01G1/00A01G7/06C12R1/125C12R1/07C12R1/82
CPCA01G7/06A01G22/00A01N63/00A01N63/30C12N1/14C12N1/20A01N2300/00
Inventor 赵树兰多立安高星星
Owner TIANJIN NORMAL UNIVERSITY
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