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Compound microorganism bacterium agent of biological coalbed methane prepared by coal bed organic impurities and application thereof

A compound microbial agent and bio-coal bed gas technology, applied in the direction of micro-organisms, micro-organism-based methods, biochemical equipment and methods, etc., can solve the problem of unknown ratio and synergy between strains, operation intervention of coal-bed methane production process, and lack of stability Sexuality and other problems, to achieve the effect of shortening the anaerobic digestion time, good effect and long action time

Inactive Publication Date: 2013-04-24
江苏加德绿色能源有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of the microorganisms involved in the above patents has a clear strain number, and the microorganisms used are domesticated and lack stability; the ratio and synergy between the strains are unknown; and there is no intervention in the operation of the coalbed methane production process

Method used

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  • Compound microorganism bacterium agent of biological coalbed methane prepared by coal bed organic impurities and application thereof
  • Compound microorganism bacterium agent of biological coalbed methane prepared by coal bed organic impurities and application thereof
  • Compound microorganism bacterium agent of biological coalbed methane prepared by coal bed organic impurities and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0034] Example 2 Utilizing a composite microbial bacterial agent to treat coalbed methane produced by anaerobic fermentation of coal in Inner Mongolia

[0035] 1, four kinds of microbial fermented liquids prepared in embodiment 1 are mixed uniformly by following weight parts and become compound microbial inoculum: 8 weight parts of Phanerochaete chrysosporium fermented liquids, 5 weight parts of Penicillium chrysogenum fermented liquids, Pseudomonas cepacia 10 parts by weight of bacteria fermentation liquid, 7 parts by weight of Clostridium acetobutylicum fermentation liquid;

[0036] 2. Select 50kg of coal from a coal mine in Inner Mongolia (sealed quickly after on-site sampling), divide it into 5 parts on average, and add them to 5 15L anaerobic reaction tanks respectively;

[0037] 3. Add 80ml of the following microbial fermentation liquid or microbial fermentation liquid mixture to the four anaerobic reaction tanks respectively: a: Phanerochaete chrysosporium fermentation ...

Embodiment 3

[0042] Example 3 Utilizing composite microbial bacterial agent to process coal bed methane produced by anaerobic fermentation of coal in Gansu

[0043] 1. Replace the 4 microorganisms in Example 1 with the following sources: Phanerochaete chrysosporium (CICC40719), Penicillium chrysogenum (ACCC32009), Pseudomonas cepacia (CICC20699), Clostridium acetobutylicum (CGMCC1.135); Prepare four kinds of microbial fermented liquids according to the method of Example 1, and mix uniformly by the following parts by weight to become composite microbial inoculum I: 11 parts by weight of Phanerochaete chrysosporium fermented liquid, Penicillium chrysogenum fermented liquid 6 parts by weight, 8 parts by weight of Pseudomonas cepacia fermentation broth, 14 parts by weight of Clostridium acetobutylicum fermentation broth; mix uniformly according to the following parts by weight to become composite microbial bacterial agent II: Phanerochaete chrysosporium fermentation broth 26 The parts by weigh...

Embodiment 4

[0051] 1. Replace Phanerochaete chrysosporium (ATCC20696) in Example 1 with Phanerochaete chrysosporium (CICC40719), Clostridium acetobutylicum (ATCC824) with Clostridium acetobutylicum (CGMCC1 .135), prepare four kinds of microbial fermented liquids according to the method of Example 1, and mix uniformly according to the following proportions to become composite microbial inoculum I: 6 parts by weight of Phanerochaete chrysosporium fermented liquid, 4 parts by weight of Penicillium chrysogenum fermented liquid , 12 parts by weight of Pseudomonas cepacia fermented liquid, 12 parts by weight of Clostridium acetobutylicum fermented liquid; mix uniformly by the following parts by weight to become composite microbial bacterial agent II: 7 parts by weight of Phanerochaete chrysosporium fermented liquid, 6 parts by weight of Penicillium chrysogenum fermentation liquid, 2 parts by weight of Pseudomonas cepacia fermentation liquid, and 23 parts by weight of Clostridium acetobutylicum f...

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Abstract

The invention belongs to the field of preparing biological coalbed methane by microorganisms, and discloses a compound microorganism bacterium agent of the biological coalbed methane prepared by coal bed organic impurities and an application of the compound microorganism bacterium agent. The compound microorganism bacterium agent comprises fermentation broths of four bacteria namely Phanerochaetechrysosporium, Penicilliumchrysogenum, Pseudomonasecpacia and Clostridiumacetobutylium. The compound microorganism bacterium agent disclosed by the invention has strong digestive ability to various celluloses, humus and various carbon-containing macromolecules in the coal bed. A coal added with the compound microorganism bacterium agent can accelerate the enrichment of methanogenus, shorten anaerobic digestion time and improve the output of the biological coalbed methane; and in addition, the synergy effects of the four microorganism strains are superior to the effect of any one, two or three microorganisms.

Description

technical field [0001] The invention belongs to the field of producing biological coal-bed methane by microorganisms, and relates to a composite microbial bacterial agent for producing biological coal-bed methane by utilizing coal bed organic impurities and an application thereof. Background technique [0002] Bio-coal bed methane, commonly known as gas, is a gas with methane as the main component produced by the bio-organic impurities contained in the coal seam under suitable environmental conditions, through the hydrolysis, acidification and methanation of a series of microorganisms, and the physical and chemical effects of high temperature and high pressure. , and a small amount of ethane, propane and butane, as well as hydrogen sulfide, carbon dioxide, nitrogen and water vapor, most of which come from biological effects, so it is also called biological coalbed methane. When the concentration of biological coalbed methane is between 5% and 16%, it will explode in case of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14E21B43/22C12R1/82C12R1/38C12R1/145C12R1/645
CPCY02E50/30
Inventor 周楚新李登奎支银芳程苏宁
Owner 江苏加德绿色能源有限公司
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