Compound microorganism combination microbial agent, preparation method and application thereof, comprehensive treatment method for black-odor river

A technology of compound microorganisms and combined inoculants, which is applied in the field of compound microorganisms, combined inoculants, and comprehensive treatment of black and odorous rivers, can solve the problems of insignificant hydrogen sulfide elimination, weak degradation ability, and low treatment efficiency.

Active Publication Date: 2017-11-28
安徽瑞驰兰德生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the application of single microbial preparations in the treatment of black and odorous water bodies, there are often problems such as weak degradation ability to nitrogen, phosphorus and organic matter and other pollutants, low treatment efficiency, and ineffective elimination of odorous gas hydrogen sulfide in black and odorous water bodies.

Method used

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  • Compound microorganism combination microbial agent, preparation method and application thereof, comprehensive treatment method for black-odor river

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Embodiment 1

[0065] A compound microbial combination bacterial agent, including bacterial agent A combination, bacterial agent C combination and bacterial agent γ combination;

[0066] The preparation method of described bacterial agent A combination is:

[0067] (1) Inoculate Bacillus coagulans, lactic acid bacteria, saccharomyces, Aspergillus sojae and Thiobacillus denitrifica in liquid medium respectively, and cultivate them at 28~32° C. for 48 hours; the liquid medium contains the following components per liter of medium: 8g of beef extract, 10g of peptone, 3g of yeast extract, 5g of sodium chloride, 1g of potassium dihydrogen phosphate, 0.1g of trace elements, 30g of sucrose, and the rest are distilled water with a pH of 6.8 to 7.2; the trace elements are Mg 2+ or Fe 2+ , all used are the corresponding sulfates;

[0068] (2) Place the strains cultivated in step (1) in fermenters respectively, mass-produce and cultivate them at 28-32°C for 48 hours, and stir the sterile air for 2 min...

Embodiment 2

[0098] A compound microbial combination bacterial agent, including bacterial agent A combination, bacterial agent C combination and bacterial agent γ combination;

[0099] The preparation method of described bacterial agent A combination is:

[0100] (1) Inoculate Bacillus coagulans, lactic acid bacteria, saccharomyces, Aspergillus sojae and Thiobacillus denitrifica in liquid medium respectively, and cultivate them at 28~32° C. for 48 hours; the liquid medium contains the following components per liter of medium: 8.8g of beef extract, 11g of peptone, 3.3g of yeast extract, 5.5g of sodium chloride, 1.1g of potassium dihydrogen phosphate, 0.11g of trace elements, 33g of sucrose, and the rest are distilled water with a pH of 6.8 to 7.2; the trace elements are Mg 2+ or Fe 2+ , all used are the corresponding sulfates;

[0101] (2) Place the strains cultivated in step (1) in fermenters respectively, mass-produce and cultivate them at 28-32°C for 48 hours, and stir the sterile air ...

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Abstract

The invention discloses a compound microorganism combination microbial agent, a preparation method and application thereof, a comprehensive treatment method for a black-odor river. The compound microorganism combination microbial agent comprises the three kinds of microbial agent combinations of a microbial agent A combination, a microbial agent C combination and a microbial agent gamma combination, and the microbial agent A combination comprises the microbial strains of bacillus coagulans, lactic acid bacteria, saccharomycetes, aspergillus sojae and thiobacillus denitrificans; the microbial agent C combination comprises the microbial strains of bacillus coagulans, rhizopus oryzae, saccharomycetes, aspergillus sojae and penicillium chrysogenum; and the microbial agent gamma combination comprises the microbial strains of lactic acid bacteria, bacillus subtilis, saccharomycetes, bacillus licheniformis and bacillus coagulans. The compound microorganism combination microbial agent can be used for comprehensive treatment of the black-odor river, the treatment period is short, the situation that nitrogen and phosphorus of a black and odorous water body exceed a standard can be solved, and odorous gas hydrogen sulfide in the water body can be eliminated.

Description

technical field [0001] The invention belongs to the field of sewage treatment, and in particular relates to a compound microbial composition bacterial agent, its preparation method and application, and a method for comprehensive treatment of black and odorous river courses. Background technique [0002] Urban water bodies such as rivers are an important part of urban ecosystems. They have the characteristics of small water environment capacity, poor water self-purification ability, and easy pollution. They are often used as important sewage discharge and sewage channels in cities, and water quality is more likely to deteriorate. The influx of a large amount of pollutants has led to serious eutrophication of urban water bodies, resulting in a sharp consumption of dissolved oxygen in water bodies, resulting in long-term hypoxia in water bodies and resulting in black and odorous states. Black and odorous water bodies in cities such as rivers have become a common phenomenon in C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C02F3/34C02F11/02C02F3/32C12R1/07C12R1/845C12R1/82C12R1/125C12R1/10C12R1/66C12R1/01C02F103/00
CPCC02F3/327C02F3/341C02F3/347C02F11/02C02F2103/007C12N1/14C12N1/16C12N1/20
Inventor 徐钰青芮文东刘晓龙陈森林
Owner 安徽瑞驰兰德生物科技有限公司
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