Complex bacterial preparation for degrading non-wood-fiber lignin and preparation method thereof

A non-wood fiber and composite bacterial agent technology, which is applied in the field of lignin rapid degradation bacterial agent and lignin composite bacterial agent, can solve the problems of lignin refractory degradation, low efficiency, long time, etc.

Inactive Publication Date: 2014-09-10
INNER MONGOLIA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] This invention aims at the problems of difficult degradation of lignin in papermaking raw materials, long time, low efficien...

Method used

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  • Complex bacterial preparation for degrading non-wood-fiber lignin and preparation method thereof
  • Complex bacterial preparation for degrading non-wood-fiber lignin and preparation method thereof
  • Complex bacterial preparation for degrading non-wood-fiber lignin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Aspergillus fumigatus is numbered A, Penicillium chrysogenum is numbered B, and Penicillium vulgaris is numbered C. Configure 300mL PDA culture based on a 500mL triangular flask, use the inoculation loop to pick out 3-5 rings of each of the three strains of A, B, and C, and culture single bacteria. Inoculate in liquid PDA medium at a ratio of 1:1 and 1:1:1, culture on a shaker at 30°C and 170rpm, and after three days of cultivation, put 10g of sterilized reed into it, take samples every two days, and measure Lignin content, such as figure 1 shown. from figure 1 It can be seen that B bacteria has the best degradation effect of a single species of bacteria, and the degradation rate is 38.8%. The best degradation effect of the two bacteria is the combination of BC, with a degradation rate of 43.02%. Well, the degradation rate reached 57.59%, and the degradation of reed lignin by the mixture of three bacteria was significantly better than that by single bacteria or by th...

Embodiment 2

[0023] Aspergillus fumigatus is numbered A, Penicillium chrysogenum is numbered B, and Penicillium vulgaris is numbered C. Configure 300mL PDA culture based on a 500mL triangular flask, use the inoculation loop to pick out 3-5 rings of each of the three strains of A, B, and C, and after three days of cultivation, according to (bacterial liquid volume ratio) A:B:C=1:1 Inoculate 10 mL in different amounts (100 mL, 150 mL, 200 mL, 250 mL, 300 mL) of liquid PDA medium at a ratio of 1:1, cultivate on a shaker at 30°C and 170 rpm, and after three days of cultivation, 10 g of sterilized reed Put it in, take a sample every two days, measure the lignin content, such as figure 2 shown. from figure 2 It can be seen that when the bacterial liquid is 200mL, the degradation effect is the best, and the degradation effect is the best on the tenth day, the lignin content is 13.55%, and the degradation rate is 58.41%.

Embodiment 3

[0025] Aspergillus fumigatus is numbered A, Penicillium chrysogenum is numbered B, and Penicillium vulgaris is numbered C. Configure 200mL PDA culture based on a 500mL triangular flask, use the inoculation loop to pick out 3-5 rings of each of the three strains of A, B, and C, and after three days of cultivation, on the basis of a BC mixing ratio of 1:4 (bacteria liquid volume ratio) Above, inoculate with A in 200 mL liquid PDA medium according to different mixing ratios (bacteria liquid volume ratio: 1:1, 1:2, 1:3, 1:4, 1:5), at 30°C, 170rpm shaker cultivation, after cultivation for three days, put 10g sterilized reeds into it, take a sample every two days, measure the lignin content, such as image 3 shown. from image 3 It can be seen that on the basis of the BC bacteria mixing ratio of 1:4, the mixing ratio of the three ABC bacteria is optimized, and the volume ratio of the ABC mixed bacteria is A:B:C=1:4:16, and on the 8th day, the degradation The effect is the best, t...

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Abstract

The invention discloses a complex bacterial preparation for degrading non-wood-fiber lignin and a preparation method thereof. The preparation method is characterized by comprising the steps of inoculating and culturing aspergillus fumigatus, penicillium chrysogenum and common penicillium, wherein the inoculating ratio of the aspergillus fumigatus, the penicillium chrysogenum and the common penicillium is 0.5-2:3-5:15-17; carrying out mixed culture for degrading reed lignin. According to the invention, through the mixed culture of strains, complementary and synergistic effects exist between strains, so that the conversion rate is improved in effective time, and the efficiency of reed lignin degradation can be greatly improved.

Description

technical field [0001] The present invention relates to a compound microbial agent for degrading non-wood fiber lignin and a preparation method thereof, specifically, a lignin rapid degradation bacterial agent for reed, bamboo, straw and other papermaking raw materials. The present invention relates to said A method for degrading a lignin composite bacterial agent and its application. Background technique [0002] Non-wood fiber refers to papermaking raw materials other than wood, including bamboo, reed, sugarcane, straw and waste paper. Take the rich reed in Wuliangsuhai, Inner Mongolia as an example. Reed is an aquatic or wet perennial gramineous plant. It mostly grows in watery areas such as ditches, swamps, rivers and streams, and is widely distributed in my country. In Wuliangsuhai, Inner Mongolia, the reed area has reached 120 square kilometers, accounting for 2 / 5 of the entire lake area, and the harvesting output has reached 100,000 tons. It contains 44% cellulose, sec...

Claims

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Application Information

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IPC IPC(8): C12N1/14D21C5/00C12R1/68C12R1/82C12R1/80
Inventor 卢庆华蔡禄卢俊邢孟兰王蕾季祥
Owner INNER MONGOLIA UNIV OF SCI & TECH
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