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Penicillium chrysogenum CH03 strain and application thereof in restoring heavy metal pollution in soil

A technology for soil remediation and production of Penicillium chrysogenum, applied in the restoration of polluted soil, fungi, microorganism-based methods, etc., can solve the problems of reducing the proportion of water-soluble and exchangeable heavy metals, and reducing the bioavailability of heavy metals, etc. Achieving the effect of low cost, simple operation and good repair effect

Active Publication Date: 2014-03-12
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, because straw contains a large amount of lignin and cellulose, it is an important source of soil organic matter, especially humus, and the latter can effectively adsorb and complex heavy metal ions, reducing the water solubility and solubility in soil. The proportion of heavy metals in the exchange state reduces the bioavailability of heavy metals

Method used

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  • Penicillium chrysogenum CH03 strain and application thereof in restoring heavy metal pollution in soil
  • Penicillium chrysogenum CH03 strain and application thereof in restoring heavy metal pollution in soil
  • Penicillium chrysogenum CH03 strain and application thereof in restoring heavy metal pollution in soil

Examples

Experimental program
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Effect test

Embodiment 1

[0022] The isolation and identification of embodiment 1 Penicillium chrysogenum CH03 bacterial strain

[0023] Weigh 10g of chicken manure compost provided by Wuhan Yuzhong Biological Engineering Co., Ltd. into a conical flask filled with 90mL of sterile saline, shake it on a constant temperature shaker at 28°C at 150r / min for 10 minutes, let it stand for 1 minute, and take 1mL The supernatant was transferred to 9 mL of sterilized normal saline, mixed and then serially diluted 10 times to 10 -3 . Take 100 μL of dilutions of different concentrations and spread them in the pre-poured fungal selection medium (containing 1-10mmol / L Cd 2+ and 50 μg / mL kanamycin), after standing for a while, the plate was inverted and placed in a constant temperature incubator at 28°C for 5-7d. The composition of the fungal culture medium is: KH per liter of water 2 PO 4 0.2g, KH 2 PO 4 0.6g, MgSO 4 ·7H 2 O0.2g, CaSO 4 0.1g, (NH 4 ) 2 SO 4 5g, yeast extract 1g, glucose 20g, pH5.5, agar 2...

Embodiment 2

[0024] Application of Example 2 Penicillium chrysogenum CH03 in remediation of soil heavy metal pollution

[0025] 1. Penicillium chrysogenum CH03 spore expansion culture

[0026] Inoculate Penicillium chrysogenum CH03 into the PDA agar slant medium, and culture it in a constant temperature incubator at 28°C for 5-7 days. After a large number of spores have been produced, wash the PDA slant with sterile water, and transfer the spore washing solution to a sterile 1.5ml In a centrifuge tube, centrifuge at 10000r / min at 4°C for 10min, discard the supernatant, resuspend the spores with an appropriate amount of sterile water, and adjust the concentration of the spores to 2×10 9 / ml, stored at 4°C for later use.

[0027] 2. Rice straw pretreatment

[0028] Select rice straw from the farmland not polluted by heavy metals in Huazhong Agricultural University, Wuhan City, Hubei Province, expose it to the sun to remove water, and then crush it to a length of 1-2 cm with a small-scale a...

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Abstract

The invention belongs to the field of restoration of heavy metal pollution in soil, and discloses a Penicillium chrysogenum strain. The strain is collected at China Center for Type Culture Collection, and the collection number is CCTCC NO:M2013604. The invention also discloses application of the Penicillium chrysogenum CH03 strain in restoring heavy metal pollution in soil and a method for restoring heavy metal pollution in soil. The method can enhance the yield of lettuce and wheat growing in a heavy-metal-polluted field and lower the contents of copper and cadmium in plant tissues, and thus, is a method for restoring heavy-metal-polluted soil in situ by using a microbe-organic substance combination. The method is simple to operate, has the advantages of low cost, no need of stopping farming and favorable restoration effect, and thus, is an environment-friendly method for restoring heavy-metal-polluted soil.

Description

technical field [0001] The invention belongs to the field of remediation of soil heavy metal pollution, and in particular relates to a strain of Penicillium chrysogenum CH03 and its application in remediation of soil heavy metal pollution. technical background [0002] Due to mining and smelting, sewage irrigation, and large-scale application of pesticides and fertilizers, the area of ​​cultivated land polluted by heavy metals in my country is nearly 20 million hectares, accounting for about 1 / 5 of the total cultivated land area. Heavy metals cannot be degraded, and can pollute groundwater after accumulating in soil, or threaten the health of humans and animals through the food chain. Heavy metal immobilization restoration has attracted more and more attention because of its advantages of simple operation, low cost, and easy realization of in-situ restoration. It is especially suitable for the restoration of farmland with medium and low heavy metal pollution. In situ immobi...

Claims

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Application Information

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IPC IPC(8): C12N1/14B09C1/10C12R1/82
Inventor 黄巧云陈雯莉李明徐兴健
Owner HUAZHONG AGRI UNIV
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