42 results about "Soma" patented technology

The invention relates to the field of cytobiology and neurobiology, in particular to an induction medium for inducing neural stem/progenitor cells to be differentiated into oligodendrocyte precursor cells and an induction method and application thereof. The invention adopts the technical scheme that after subjected to preprocessing culture for a certain time by a preprocessing medium, the human neural stem/progenitor cells are rapidly differentiated into the oligodendrocyte precursor cells for expressing oligodendrocyte precursor cell markers such as O4, A2B5, NG2, SOX10, PDGFR (Platelet Derived Growth Factor Receptor) and the like at a high purity under the action of the induction medium; and the key ingredients of the induction medium are bFGF (Basic Fibroblast Growth Factor), PDGF-AA and NT-3 (neurotrophins-3). The method disclosed by the invention and the obtained oligodendrocyte precursor cells can be applied to preparation of a medicament for treating the nervous system injury disease and have wide prospect on the aspects of experiment research and clinical treatment.

The present invention describes a dissociated cell culture system comprising cells of the hippocampus, one of the brain areas affected by Alzheimer's Disease (AD) or amyloid beta-related diseases. This culture system comprises hippocampal neuronal and glial cells from animal models of AD, particularly, but not limited to, double transgenic mice expressing both the human APP mutation (K670N:M671L) (mAPP), and the human PS1 mutation (M146L) (mPS1), and serves as a powerful tool for the screening and testing of compounds and substances, e.g., drugs, for their ability to affect, treat, or prevent AD or β-amyloid-related diseases. The effects of a test substance on the cells in this culture system can be quantitatively assessed to determine if the test substance affects the cells biochemically and/or electrophysiologically, and/or optically, and/or immunocytochemically. The present in vitro culture system is advantageous for AD drug screening, because it is rapid and efficient. By contrast, even in the fastest animal model of AD, pathology does not start before the end of the second month. If such in vivo animal models are used, it is necessary to wait at least the two month time duration or longer to test for drug efficacy for AD treatment or prevention. At the same time the present invention provides a tool for production of amyloid-beta that can be used for electrophysiological, behavioral, and toxicological studies.

The invention discloses a method for inducing human or animal somatic cells to neural stem cells and purpose of neural stem cells obtained by the method. By overexpression of transcription factor Ptf1a in separated human or animal somatic cells and culture in a culture medium containing EGF and bFGF, somatic cells can be induced to neural stem cells (iNSCs). The iNSCs obtained by the method has self-renewing capability, and can stably passage for 50 generations and above and maintain original cell characteristics. By culture in different in vitro differentiation mediums, the iNSCs can be efficiently differentiated to neurons, astrocytes and oligodendrocytes. By injecting the iNSCs in animal bodies, the injected cells can be integrated into nervous tissues of retina, cerebrum, etc. the method of the invention is simpler, safer and more efficient than previous methods. The iNSCs obtained by the method and further differentiated cells can be applied to cell models of nerve diseases, drug screening, stem cell treatment and the like.

The invention discloses a liver and bile cancer cell postoperative monitor based on overall association medical logic, which comprises a special earphone worn on the two ears of a patient, and the special earphone comprises a transmitter and a receiver; the special earphone is electrically connected with a noise voltage generator, and the noise voltage generator is connected with a processor through a differential amplifier; the processor is connected with the pulse width modulation circuit; the invention further discloses a use method of the liver and bile cancer cell postoperative monitor based on the overall association medical logic, and the use method comprises the steps that S1, a detected person wears the specially-made earmuff, electromagnetic waves are emitted through the left earmuff and reach the cerebral cortex, and an external magnetic field is generated through electromagnetic resonance; the method is simple, the neurons can be stimulated to generate chemical electric signals, the chemical electric signals are transmitted to tissue cells of organs of the whole body through the neurons to cause resonance, and the disease state and degree of the human cell level are judged after analysis so as to count significance and predict clinical feature significance.

The invention discloses a method for treating child cerebral palsy through neural precursor cells. The method comprises the following steps: preoperative preparation, cell transplantation and postoperative treatment. The NPC in the invention is wide in source and convenient in collection, has high multiplication capacity and high cell viability, also has excellent differentiation regulation property and multi-potential differentiation, and can serve as a good carrier for gene therapy. Meanwhile, due to the test performed by the method, gross motor functions, such as lying and turning, sitting,walking and initiative grabbing and hand-eye coordination abilities, of cerebral palsy children treated within four weeks can be obviously improved. Therefore, according to the method disclosed by the invention, the cerebral injury process can be directly inhibited or regeneration of injured nerves can be promoted.

The invention relates to the technical field of biology, in particular to a method for amplifying human nerve precursor cells through regulation of Wnt signals and/or Notch signals. The culture methodof the human neural precursor cells comprises the step of culturing the human neural precursor cells, of which Wnt signals and/or Notch signals are exogenously regulated, under proper conditions. Ina culture system of directionally differentiated human neural cells, exogenous activation, inhibition or over-expression is performed on the Wnt signals and/or Notch signals, so that the development process of the neural precursor cells is interfered exogenously, the proliferation ability of the neural precursor cells is enhanced, and large-scale and long-time proliferation of the neural precursorcells is promoted; and meanwhile, the normal differentiation potential of the neural precursor cells into multiple specific types of neurons is maintained, and therefore, the method is provided for in-vitro and large-scale culture of the neural precursor cells which have continuous proliferation ability and normal neural differentiation potential.

The invention provides a method for efficiently inducing human cells to be reprogrammed into neuronal cells. The concentration of cAMP is improved or the expression of PKA and CREB is up-regulated or the expression of AMPK, ALK2, ALK3, P38 and JNK is inhibited through a single small molecule compound or gene knockout or gene overexpression. The induced small molecule compound is single and safe, short in induction time, high in induction efficiency, clear in induction action site and gene and clear in molecular regulation mechanism, can be applied to clinical treatment of human neurodegenerative diseases, and provides a safer and more efficient treatment means for treatment of the neurodegenerative diseases. As neurons do not have division and proliferation capacities, fibroblasts and astrocytes with division and proliferation capacities can be induced in vivo and in vitro by utilizing the method; and a large number of induced neurons can be continuously obtained in vivo and in vitro.

The invention discloses a spherical relay coil and a wireless power transmission system. The whole spherical relay coil is divided into four different coil groups which are respectively represented as axons, dendrites, synapses and somatic cells of neurons, and a three-dimensional spherical structure shell of the neurons comprises an axon coil and a dendritic coil which are respectively used as an energy transmission part and an energy receiving part. Somatic cell coils are arranged in the three-dimensional spherical structures of the neurons, and the somatic cell coils are used as relay coils for energy transfer. According to the invention, the technical problems of low wireless power transmission utilization rate, limited position and low spatial degree of freedom in the prior art are solved.

The present invention provides methods of identifying and distinguishing different types of nerve cells in ex vivo cell culture, the method comprising the steps of: a) culturing somato-sensory nerve cells ex vivo, b) loading the nerve cells with a calcium, sodium, or voltage-sensitive indicator or expressing a genetically encoded calcium, sodium, or voltage-sensitive indicator, c) pulsing the nerve cells with an electrical train of bipolar square waves at two different voltages and two different frequencies; wherein the first voltage is 10 V/cm or less (low voltage) and the second voltage is between 12 and 20 V/cm (high voltage); and wherein the first frequency is 5 Hz or less (low frequency) and the second frequency is between 15 and 20 Hz (high frequency), and d) detecting activation of the nerve cell by measuring the changes in the signal intensity of the indicator, wherein low voltage and low frequency activation indicates a first type of cell and activation detected only at high voltage indicates a second type of cell.

The invention discloses a children infantile autism cranial neuron cell assessment instrument based on big data, which comprises a special earphone worn on the two ears of a child patient, the special earphone comprises a transmitter and a receiver, the special earphone is electrically connected with a central processing unit, and the central processing unit is connected with a medical analysis system and a big data system. And diseases are monitored through a medical analysis system and a big data system. The invention further discloses a use method of the child autism cerebral neuron cell evaluation instrument based on the big data, and the method comprises the steps that S1, a detected person wears the specially-made earmuff, electromagnetic waves are emitted through the left earmuff and reach the cerebral cortex, and an external magnetic field is generated through electromagnetic resonance; the method is simple, the neurons can be stimulated to generate chemical electric signals, the chemical electric signals are transmitted to tissue cells of organs of the whole body through the neurons to cause resonance, the disease state and degree of the human body cell level are judged after analysis, and a good monitoring effect is achieved on brain neuron cells of autistic children.

The invention relates to an application of a hydrogen sulfide slow-release organic donor ADT-OH drug in neural precursor cell differentiation, and the substance can induce more directional differentiation of neural precursor cells into neurons and oligodendrocytes and less differentiation into astrocytes, promote the axon growth of neurons, promote the apoptosis of neurons. Meanwhile, the death number of the neural precursor cells can be reduced, a direction is provided for repairing damaged nerves by transplanting the neural precursor cells, and ADT-OH is likely to become a new drug target for clinically treating nervous system diseases.

The neural cell population of the present invention is characterized in that in the presence of F and FGF-2, the cells are in an adherent monolayer culture, and at least 80% of the cells are symmetrically divided neural stem cells, No more than 1% of the cells express markers of mature astrocytes, neurons, or oligodendrocytes, and they express the markers RC2, 3CB2, and BLBP, and neural stem cells in said population are also characterized in that they express At least one of the proteins: GLAST, Pax‑6, neural progenitor cell markers stem protein or vimentin, LewisX antigen, Musashi‑l or convexin. The preparation method of the invention is simple and easy to operate.

The invention provides application of compounds in preparation of a medicine for treating and/or preventing demyelination diseases. The compounds of a formula I to a formula V can be used for promoting differentiation and myelination of oligodendroglia precursor cells to oligodendroglia cells by inhibiting TM7SF2 to improve accumulation of FF-MAS in a cholesterol metabolic pathway; and original oligodendroglia precursor cells in a brain of a patient suffering from the demyelination diseases are promoted to be differentiated into the oligodendroglia cells to form myelin sheaths to wrap axons again, so that neurological function decline caused by the loss of the myelin sheaths is recovered, and the compounds have the effects of treating and/or relieving and/or preventing the demyelination diseases.

The invention discloses a method of application of taurine in neuron proliferation of IUGR fetal rats and belongs to the field of new use of drugs. The method comprises the steps of mating rats according to a gender ratio, then, dividing impregnated rats into a normal control group, an IUGR model group, a taurine treatment group and an irradiation experimental group, then, inhibiting regeneration of neurons of the irradiation experimental group by using a radiotherapy method, observing influence on the quantity of neural precursor cells caused by irradiation, then, marking the neural precursor cells, carrying out taurine treatment, and acquiring and counting hippocampal dentate positive cells. According to the technical scheme, new use of the taurine is discovered; and by using actions of a sulfonic group in the taurine, the taurine can have relatively low lipophilicity, difficultly infiltrate into a blood-brain barrier and achieve intracerebral high-concentration physiological action exerting by mainly depending on a transport protein of the taurine.

The invention, in some aspects, relates to polypeptide molecules and their encoding nucleic acid molecules and use of such molecules to direct and localize sensor molecules in the soma of cells in which they are expressed. Compositions of the invention may be delivered to cells and subjects and used in methods to determine activity in cells in which they are expressed.