49 results about "Soma" patented technology

The present inventors discovered that brain-localizing cells exist in the progenitor cell fraction of bone marrow cells. They also elucidated various characteristics of the brain-localizing cells. When these cells are infused into the bloodstream, they circulate in the blood and directly translocate into the cerebral parenchyma from the bloodstream. Furthermore, the present inventors succeeded in developing methods for efficiently preparing brain-localizing cells from bone marrow or bone marrow cells. These methods can be applied to less-invasive regenerative medicines targeting the brain and using autologous cells.

Provided are cortical interneurons and other neuronal cells and in vitro methods for producing such cortical interneurons and other neuronal cells by the directed differentiation of stem cells and neuronal progenitor cells. The present disclosure relates to novel methods of in vitro differentiation of stem cells and neural progenitor cells to produce several type neuronal cells and their precursor cells, including cortical interneurons, hypothalamic neurons and pre-optic cholinergic neurons. The present disclose describes the derivation of these cells via inhibiting SMAD and Wnt signaling pathways and activating SHH signaling pathway. The present disclosure relates to the novel discovery that the timing and duration of SHH activation can be harnessed to direct controlled differentiation of neural progenitor cells into either cortical interneurons, hypothalamic neurons or pre-optic cholinergic neurons. The present disclosure also relates to compositions of cortical interneurons, hypothalamic neurons or pre-optic cholinergic neurons, and their precursors, that are highly enriched and can be used in variety of application. These cells can be used therapeutically to treat neurodegenerative and neuropsychiatric disorders, and can be used for disease modeling and drug screening.

The invention relates to the field of cytobiology and neurobiology, in particular to an induction medium for inducing neural stem / progenitor cells to be differentiated into oligodendrocyte precursor cells and an induction method and application thereof. The invention adopts the technical scheme that after subjected to preprocessing culture for a certain time by a preprocessing medium, the human neural stem / progenitor cells are rapidly differentiated into the oligodendrocyte precursor cells for expressing oligodendrocyte precursor cell markers such as O4, A2B5, NG2, SOX10, PDGFR (Platelet Derived Growth Factor Receptor) and the like at a high purity under the action of the induction medium; and the key ingredients of the induction medium are bFGF (Basic Fibroblast Growth Factor), PDGF-AA and NT-3 (neurotrophins-3). The method disclosed by the invention and the obtained oligodendrocyte precursor cells can be applied to preparation of a medicament for treating the nervous system injury disease and have wide prospect on the aspects of experiment research and clinical treatment.

This invention provides platelet-derived growth factor-responsive neural precursor (PRP) cells and methods of producing such cells in vivo or in vitro. These cells can further be used to generate neurons, oligodendrocytes and / or astrocytes.

The invention discloses a method of induced neural stem cells and a composition. Through the method and the composition, peripheral blood mononuclear cells can be induced to form neural stem cells. The neural stem cells can express neural stem cell related genes, and can disintegrate out nerve cells, astroglia cells and oligodendrocyte. Dopaminergic nerve precursor cells formed through in vitro induction and disintegration of the neural stem cells are transplanted into the striatum corpora of a PD mouse model, no tumors are formed, the ethology of a mouse Parkinson 's disease model can be improved, and the process of the Parkinson 's disease can be delayed. The neural stem cell inducing and disintegrating method provided by the invention is simple and quick to operate, small in traumatic occlusion and good in safety, and is hopefully used for treating the Parkinson 's disease.

Methods for producing a population of human-derived glial restricted progenitor cells (GRPs) with decreased potentially unintended or undesired cellular phenotypes and / or decreased standard deviation in the cells of the population are provided. Also provided are antibody panels and gene expression profiles to characterize GRPs and a method for its use in characterizing GRP cells. In addition methods for use of these GRP cells to generate astrocytes and / or oligodendrocytes, to re-myelinate neurons and to treat glial cell related and other neurodegenerative diseases or disorders or injuries or damage to the nervous system are provided. A method to manufacture neural cells depleted of A2B5 positive cells is also provided.

A method of preparing neural precursor cells by exposing pluripotent stem cells or neural stem cells to a differentiation agent. The agent is a pyridine analog, which in preferred embodiments is a phenylethynyl-substituted or phenylazo-substituted pyridine. In other embodiments, a method of enhancing neural precursor cell survival is provided in which the survival is enhanced by exposure to the pyridine analog. In further embodiments, a method of preparing neuronal cells is provided in which pluripotent or neural stem cells exposed to the pyridine analog are then incubated without the pyridine analog, resulting in differentiation into neurons, astrocytes and oligodendrocytes. These methods may be used in toxicological screens, e.g., to evaluate the neurotoxicity of a test compound.

The present invention describes a dissociated cell culture system comprising cells of the hippocampus, one of the brain areas affected by Alzheimer's Disease (AD) or amyloid beta-related diseases. This culture system comprises hippocampal neuronal and glial cells from animal models of AD, particularly, but not limited to, double transgenic mice expressing both the human APP mutation (K670N:M671L) (mAPP), and the human PS1 mutation (M146L) (mPS1), and serves as a powerful tool for the screening and testing of compounds and substances, e.g., drugs, for their ability to affect, treat, or prevent AD or β-amyloid-related diseases. The effects of a test substance on the cells in this culture system can be quantitatively assessed to determine if the test substance affects the cells biochemically and / or electrophysiologically, and / or optically, and / or immunocytochemically. The present in vitro culture system is advantageous for AD drug screening, because it is rapid and efficient. By contrast, even in the fastest animal model of AD, pathology does not start before the end of the second month. If such in vivo animal models are used, it is necessary to wait at least the two month time duration or longer to test for drug efficacy for AD treatment or prevention. At the same time the present invention provides a tool for production of amyloid-beta that can be used for electrophysiological, behavioral, and toxicological studies.

The invention discloses a method for constructing brain-like tissue by using umbilical cord mesenchymal stem cells, which comprises the following steps: 1) carrying out stem cell culture on a human umbilical cord mesenchymal stem cell line to obtain subculture cells; 2) carrying out nerve induction differentiation on the subculture cells to obtain differentiated neuronal precursor cells which areembryoid-like nerve spheres; 3) carrying out induction culture on the embryoid-like nerve spheres to obtain a neural rosettte neural tube-like structure neural spheres; and (4) carrying out differentiation culture on the neural rosettte neural tube-like structure neural spheres to obtain a human brain-like organ which contains a plurality of types of neuron mixtures. The human mesenchymal stem cells are differentiated into various neurons for the first time, the brain-like tissue is constructed, the brain-like tissue can be used as a drug screening platform, and the application prospect is wide.

The invention provides a spinal neuron damage treating agent for use in the treatment of spinal neuron damage, or an agent for promoting the proliferation of spinal neuronal precursor cells in the culture of spinal neuronal precursor cells, or an agent for promoting the regeneration of spinal nerves after transplantation of cultured spinal neuronal precursor cells, and the like.The invention provides an agent that contains a substance (e.g., ghrelin) that acts on the growth hormone secretagogue-receptor as an active ingredient, the agent being a spinal neuron damage treating agent for use in the treatment of spinal neuron damage, or an agent for promoting the proliferation of cultured spinal neuronal precursor cells in the culture of spinal neuronal precursor cells, or an agent for promoting the regeneration of spinal nerves after transplantation of cultured spinal neuronal precursor cells, and the like.

The present invention relates to a method for amplifying a population of neural precursors comprising the step of culturing neural precursors in the presence of a PKA inhibitor.

The invention discloses a method for inducing human or animal somatic cells to neural stem cells and purpose of neural stem cells obtained by the method. By overexpression of transcription factor Ptf1a in separated human or animal somatic cells and culture in a culture medium containing EGF and bFGF, somatic cells can be induced to neural stem cells (iNSCs). The iNSCs obtained by the method has self-renewing capability, and can stably passage for 50 generations and above and maintain original cell characteristics. By culture in different in vitro differentiation mediums, the iNSCs can be efficiently differentiated to neurons, astrocytes and oligodendrocytes. By injecting the iNSCs in animal bodies, the injected cells can be integrated into nervous tissues of retina, cerebrum, etc. the method of the invention is simpler, safer and more efficient than previous methods. The iNSCs obtained by the method and further differentiated cells can be applied to cell models of nerve diseases, drug screening, stem cell treatment and the like.

The invention discloses a liver and bile cancer cell postoperative monitor based on overall association medical logic, which comprises a special earphone worn on the two ears of a patient, and the special earphone comprises a transmitter and a receiver; the special earphone is electrically connected with a noise voltage generator, and the noise voltage generator is connected with a processor through a differential amplifier; the processor is connected with the pulse width modulation circuit; the invention further discloses a use method of the liver and bile cancer cell postoperative monitor based on the overall association medical logic, and the use method comprises the steps that S1, a detected person wears the specially-made earmuff, electromagnetic waves are emitted through the left earmuff and reach the cerebral cortex, and an external magnetic field is generated through electromagnetic resonance; the method is simple, the neurons can be stimulated to generate chemical electric signals, the chemical electric signals are transmitted to tissue cells of organs of the whole body through the neurons to cause resonance, and the disease state and degree of the human cell level are judged after analysis so as to count significance and predict clinical feature significance.

The invention discloses a method for treating child cerebral palsy through neural precursor cells. The method comprises the following steps: preoperative preparation, cell transplantation and postoperative treatment. The NPC in the invention is wide in source and convenient in collection, has high multiplication capacity and high cell viability, also has excellent differentiation regulation property and multi-potential differentiation, and can serve as a good carrier for gene therapy. Meanwhile, due to the test performed by the method, gross motor functions, such as lying and turning, sitting,walking and initiative grabbing and hand-eye coordination abilities, of cerebral palsy children treated within four weeks can be obviously improved. Therefore, according to the method disclosed by the invention, the cerebral injury process can be directly inhibited or regeneration of injured nerves can be promoted.

The invention relates to preparation of neuronal precursor cells, compositions comprising same and therapeutic uses.

The invention relates to the technical field of biology, in particular to a method for amplifying human nerve precursor cells through regulation of Wnt signals and / or Notch signals. The culture methodof the human neural precursor cells comprises the step of culturing the human neural precursor cells, of which Wnt signals and / or Notch signals are exogenously regulated, under proper conditions. Ina culture system of directionally differentiated human neural cells, exogenous activation, inhibition or over-expression is performed on the Wnt signals and / or Notch signals, so that the development process of the neural precursor cells is interfered exogenously, the proliferation ability of the neural precursor cells is enhanced, and large-scale and long-time proliferation of the neural precursorcells is promoted; and meanwhile, the normal differentiation potential of the neural precursor cells into multiple specific types of neurons is maintained, and therefore, the method is provided for in-vitro and large-scale culture of the neural precursor cells which have continuous proliferation ability and normal neural differentiation potential.

The invention discloses a children cerebral palsy assessment instrument based on overall association medical logic. The children cerebral palsy assessment instrument comprises a left magnetic inductor, a right magnetic inductor, an amplifying circuit, a signal transmitter, a resonator and a microprocessor, wherein a connecting headset is arranged between the left magnetic inductor and the right magnetic inductor; and the left magnetic inductor is in signal connection with the signal transmitter. The invention further discloses a use method of the children cerebral palsy assessment instrument based on overall association medical logic. The use method comprises the following specific steps of: S1, detecting process; S2, information processing; S3, analysis and assessment; and S4, logic basis. According to the children cerebral palsy assessment instrument disclosed by the invention, by setting the detection headset, the health state of human cells and a cell map are received by utilizing propagation of electromagnetic waves in a human body, and the cell state is analyzed and compared by utilizing the existing cell map, so that the information received by the brain of a cerebral palsy child patient is analyzed and checked from the microscopic cell level, and the defects in macroscopic examination are overcome.

The invention provides a method for efficiently inducing human cells to be reprogrammed into neuronal cells. The concentration of cAMP is improved or the expression of PKA and CREB is up-regulated or the expression of AMPK, ALK2, ALK3, P38 and JNK is inhibited through a single small molecule compound or gene knockout or gene overexpression. The induced small molecule compound is single and safe, short in induction time, high in induction efficiency, clear in induction action site and gene and clear in molecular regulation mechanism, can be applied to clinical treatment of human neurodegenerative diseases, and provides a safer and more efficient treatment means for treatment of the neurodegenerative diseases. As neurons do not have division and proliferation capacities, fibroblasts and astrocytes with division and proliferation capacities can be induced in vivo and in vitro by utilizing the method; and a large number of induced neurons can be continuously obtained in vivo and in vitro.

The invention discloses a spherical relay coil and a wireless power transmission system. The whole spherical relay coil is divided into four different coil groups which are respectively represented as axons, dendrites, synapses and somatic cells of neurons, and a three-dimensional spherical structure shell of the neurons comprises an axon coil and a dendritic coil which are respectively used as an energy transmission part and an energy receiving part. Somatic cell coils are arranged in the three-dimensional spherical structures of the neurons, and the somatic cell coils are used as relay coils for energy transfer. According to the invention, the technical problems of low wireless power transmission utilization rate, limited position and low spatial degree of freedom in the prior art are solved.

The invention provides a preparation method of a tissue engineered nerve graft, comprising the following steps: constructing a three-dimensional silk protein fiber porous scaffold; inoculating fibroblasts on the scaffold, and culturing and expanding the cells; adding a small molecule compound combination CFLSSVY to induce fibrogenesis Cells differentiate into neurons and form neuronal networks. The preparation method of the invention is simple and efficient, and the silk protein material used has a wide source, the source of adult cells is convenient, and the preparation is easy to be standardized. The present invention provides a bionic tissue engineered nerve graft in the true sense, which not only contains a permeable silk protein fiber scaffold that is beneficial to the growth of cells and tissues, but also contains a neuron network formed by seed cell neurons, which is a peripheral It is an ideal graft for the repair of central nervous system injury, and has broad application prospects and value.

The present invention relates to any isolated or synthetic compound, in particular a compound of general formula (I), which is capable of modulating the cellular specialization of neural stem cells, favoring the differentiation and subsequent survival of neurons and glial cells during differentiation. and differentiation of oligodendrocyte precursor cells into mature oligodendrocytes. In addition, the compounds according to the invention are able to reduce inflammatory substances in diseases affecting the nervous system, in particular by reducing the activation of microglia and / or astrocytes and / or by reducing reactive gliosis. The present invention also relates to a method for preparing the compound and the use of the compound in the preparation of a pharmaceutical composition for the prevention or treatment of diseases affecting the nervous system. More specifically, the compounds of the present invention have the following general formula (I).

The present invention provides methods of identifying and distinguishing different types of nerve cells in ex vivo cell culture, the method comprising the steps of: a) culturing somato-sensory nerve cells ex vivo, b) loading the nerve cells with a calcium, sodium, or voltage-sensitive indicator or expressing a genetically encoded calcium, sodium, or voltage-sensitive indicator, c) pulsing the nerve cells with an electrical train of bipolar square waves at two different voltages and two different frequencies; wherein the first voltage is 10 V / cm or less (low voltage) and the second voltage is between 12 and 20 V / cm (high voltage); and wherein the first frequency is 5 Hz or less (low frequency) and the second frequency is between 15 and 20 Hz (high frequency), and d) detecting activation of the nerve cell by measuring the changes in the signal intensity of the indicator, wherein low voltage and low frequency activation indicates a first type of cell and activation detected only at high voltage indicates a second type of cell.

The invention discloses a children infantile autism cranial neuron cell assessment instrument based on big data, which comprises a special earphone worn on the two ears of a child patient, the special earphone comprises a transmitter and a receiver, the special earphone is electrically connected with a central processing unit, and the central processing unit is connected with a medical analysis system and a big data system. And diseases are monitored through a medical analysis system and a big data system. The invention further discloses a use method of the child autism cerebral neuron cell evaluation instrument based on the big data, and the method comprises the steps that S1, a detected person wears the specially-made earmuff, electromagnetic waves are emitted through the left earmuff and reach the cerebral cortex, and an external magnetic field is generated through electromagnetic resonance; the method is simple, the neurons can be stimulated to generate chemical electric signals, the chemical electric signals are transmitted to tissue cells of organs of the whole body through the neurons to cause resonance, the disease state and degree of the human body cell level are judged after analysis, and a good monitoring effect is achieved on brain neuron cells of autistic children.

Provided is a method for inducing human spinal motor neuron progenitors to differentiate into spinal motor neurons, comprising the following steps: 1) culture of the spinal motor neuron progenitors: digesting the spinal motor neuron progenitors into single cells, inoculating the single cells into a coated petri dish for adherent culture with a spinal motor neuron progenitor culture medium, and culturing in an incubator at 37°C, 5% CO2 and saturated humidity; and 2) induction of the spinal motor neurons: culturing the spinal motor neuron progenitors obtained from step 1) in a basic complete medium, adding different space-time-specific signaling pathway modulating small molecules and / or growth factors at different time points to induce differentiation, culturing for 3-30 days in an incubator at 37°C, 5% CO2 and saturated humidity, changing the medium every other day.

The invention discloses a bionic micro-fluidic chip for simulating an in-vivo microorganism-intestine-brain axis signal transduction process. The micro-fluidic chip mainly comprises a top-layer chip, a nano porous membrane, a micron porous membrane and a bottom-layer chip. The micro-fluidic chip comprises an intestinal tract unit, a blood-brain barrier inflow and efflux unit and a brain unit, intestinal microorganisms and various human cells are inoculated on the chip, and signal transduction processes of metabolism, immunity, hormone and the like of microorganism-intestinal-brain axis in vivo can be simulated. The micro-fluidic chip can be used for carrying out molecular mechanism research of the action of intestinal flora and a central nervous system and screening and evaluation research of psychotropic drugs and psychotropic probiotics. According to the invention, intestinal microorganisms, the intestinal unit, the blood brain barrier and the brain unit are organically integrated on one chip, a system capable of simulating an in-vivo MGB axis signal transduction process is constructed, the problem of function simplification of a conventional chip is solved, the intestinal microorganisms are introduced, so that the chip is closer to an in-vivo real microenvironment, and the microenvironment of the chip is improved. A new therapy can be more easily found in the field of central nervous system diseases, and the development of personalized medicine is promoted.

The invention relates to an application of a hydrogen sulfide slow-release organic donor ADT-OH drug in neural precursor cell differentiation, and the substance can induce more directional differentiation of neural precursor cells into neurons and oligodendrocytes and less differentiation into astrocytes, promote the axon growth of neurons, promote the apoptosis of neurons. Meanwhile, the death number of the neural precursor cells can be reduced, a direction is provided for repairing damaged nerves by transplanting the neural precursor cells, and ADT-OH is likely to become a new drug target for clinically treating nervous system diseases.