A method for inducing the differentiation of human spinal cord motor neuron precursor cells into spinal cord motor neurons

Abstract

Provided is a method for inducing human spinal motor neuron progenitors to differentiate into spinal motor neurons, comprising the following steps: 1) culture of the spinal motor neuron progenitors: digesting the spinal motor neuron progenitors into single cells, inoculating the single cells into a coated petri dish for adherent culture with a spinal motor neuron progenitor culture medium, and culturing in an incubator at 37°C, 5% CO2 and saturated humidity; and 2) induction of the spinal motor neurons: culturing the spinal motor neuron progenitors obtained from step 1) in a basic complete medium, adding different space-time-specific signaling pathway modulating small molecules and / or growth factors at different time points to induce differentiation, culturing for 3-30 days in an incubator at 37°C, 5% CO2 and saturated humidity, changing the medium every other day.

Description

technical field [0001] The invention relates to the technical field of cell differentiation and culture. More specifically, it relates to a method for inducing the differentiation of human spinal cord motor neuron precursor cells into spinal cord motor neurons. Background technique [0002] In recent years, the number of spinal cord injury patients due to car accidents, falls, contusions and other reasons has been increasing year by year. Due to damage to the internal nerves of the spinal cord, patients with spinal cord injury generally suffer from paralysis of the lower limbs, making it difficult to take care of themselves. Under normal physiological conditions, damaged nerve cells in the human body cannot repair themselves, so traditional medical methods are difficult to completely cure the disease. Cell replacement therapy provides a new therapeutic approach for patients with spinal cord injury. Spinal motoneurons are one of the ideal cell types for disease treatment o...

AI Technical Summary

Problems solved by technology

The traditional differentiation of neural precursor cells into neurons generally has the problems of long differentiation time and low differentiation efficiency

Moreover, the use of animal-derived components and extracted proteins will be involved in the differentiation process. Animal-derived components will potentially introduce the risk of pathogenic microorganisms. It is difficult to control the uniformity of extracted protein batches, which will lead to instability of the differentiation system.

In the traditional process of differentiation of spinal cord motor nerve precursor cells into spinal cord motor neurons, the above problems also exist. The differentiation time needs at least seven days, and the differentiation efficiency is less than 40%.

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