A method for inducing the differentiation of human spinal cord motor neuron precursor cells into spinal cord motor neurons

Abstract

Provided is a method for inducing human spinal motor neuron progenitors to differentiate into spinal motor neurons, comprising the following steps: 1) culture of the spinal motor neuron progenitors: digesting the spinal motor neuron progenitors into single cells, inoculating the single cells into a coated petri dish for adherent culture with a spinal motor neuron progenitor culture medium, and culturing in an incubator at 37°C, 5% CO2 and saturated humidity; and 2) induction of the spinal motor neurons: culturing the spinal motor neuron progenitors obtained from step 1) in a basic complete medium, adding different space-time-specific signaling pathway modulating small molecules and / or growth factors at different time points to induce differentiation, culturing for 3-30 days in an incubator at 37°C, 5% CO2 and saturated humidity, changing the medium every other day.

Description

technical field

[0001] The invention relates to the technical field of cell differentiation and culture. More specifically, it relates to a method for inducing the differentiation of human spinal cord motor neuron precursor cells into spinal cord motor neurons. Background technique

[0002] In recent years, the number of spinal cord injury patients due to car accidents, falls, contusions and other reasons has been increasing year by year. Due to damage to the internal nerves of the spinal cord, patients with spinal cord injury generally suffer from paralysis of the lower limbs, making it difficult to take care of themselves. Under normal physiological conditions, damaged nerve cells in the human body cannot repair themselves, so traditional medical methods are difficult to completely cure the disease. Cell replacement therapy provides a new therapeutic approach for patients with spinal cord injury. Spinal motoneurons are one of the ideal cell types for disease treatment o...

Images