CHO cell serum-free protein-free culture medium and application thereof

Abstract

The invention relates to a CHO cell serum-free protein-free culture medium and an application thereof. The CHO cell serum-free protein-free culture medium comprises an amino acid component, a vitamincomponent, an inorganic salt component, a microelement component, carbohydrate and other molecular compound components. The serum-free culture medium provided by the invention has the advantages of definite chemical components and low cost, avoids the biological safety risk caused by the use of serum while the serum is not needed, and provides convenience for large-scale production of biological products, especially protein drugs. CHO cells grow well in the culture medium without the use of the serum, so that the stability of the production process of the biological products and the stabilityof the quality of finished products are improved, and particularly, the application of cell perfusion culture is facilitated. The cell perfusion culture technology serves as a future development trendof biomedical cell culture, and the culture medium well fills the blank that no special cell perfusion culture medium exists in the market, and provides a basis for flow line production of CHO cell biomedicines.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a serum-free and protein-free culture medium for cells. Background technique [0002] Chinese hamster ovary (CHO) cells are widely used in the production of recombinant protein drugs and antibody drugs. They can not only provide stable and accurate glycosylation modifications, making the expression products closest to natural protein molecules, but also have safety The high feature is currently one of the few legal and compliant industrialized engineering cell lines approved by the FDA and the drug regulatory authorities of various countries. Generally, the cultivation of CHO cells is completed by adding 5 to 10% fetal bovine serum to a commercial medium, such as DMEM / F12 basal medium. In addition to supplying nutrients for cells, serum also provides for the proliferation of in vitro cultured cells. necessary growth factors. However, the use of serum has many disadvantages, such as l...

AI Technical Summary

Problems solved by technology

[0004] At present, the perfusion cell culture method is becoming more and more popular, because the production process development of biopharmaceuticals has higher requirements on product quality and yield, and the perfusion cell technology can meet the quality and yield requirements, especially for unstable biological protein drugs, perfusion cells The cultivation process is the best choice, and the vast majority of biological product companies have already started or are about to start the production line of this production method, but the development or production process of the corresponding "special medium" is out of touch

Although there are already a large number of serum-free and protein-free medium with clear chemical composition, which are applied to traditional batch culture or batch-fed medium at home and abroad, there are very few products specially used in CHO cell perfusion medium.

The few types of perfusion media that have been on the market are also monopolized by well-known foreign media companies such as Life, Thermo, and Lonza, and their ingredients are kept secret. However, there is no report on the use of perfusion culture technology to develop media in China, because the perfusion cell culture method The technical difficulty is high, the medium consumption is large, and the equipment is complicated, which affects the development of the perfusion culture process

Images