T cell serum-free cryopreservation solution and using method thereof

Abstract

The invention relates to a T cell serum-free cryopreservation solution and a using method thereof. The cryopreservation solution comprises a basic culture medium, a cryopreservation protective agent and adding components which are ethanolamine, insulin, transferrin, human serum albumin, vitamin C and a cell membrane stabilizer. The cryopreservation solution disclosed by the invention has no serum,no human source and no animal source components, and is clear in chemical composition and low in DMSO content, the defect of high DMSO content in the prior art is overcome, the toxicity risk to cellsis avoided, meanwhile, possible clinical application of the cryopreservation solution is not affected, the cryopreservation solution has excellent cryopreservation performance on T cells, the resuscitation survival rate of the T cells is 90% or above after cryopreservation of the T cells, subsequent activation and amplification are not affected, and the T cell serum-free cryopreservation solutionhas high clinical application and scientific research value.

Description

technical field [0001] The invention belongs to the field of cell cryopreservation, and in particular relates to a serum-free, human-derived and animal-derived component-free T cell cryopreservation solution with a clear chemical composition and a use method thereof. Background technique [0002] T cells are one of the main components of lymphocytes. They have a variety of biological functions, such as directly killing target cells, assisting other lymphocytes to function, responding to specific antigens or mitogens, and producing cytokines. One of the body's main immune cells that fight off infection and prevent tumors from forming. Enrichment, activation, and expansion of such immune cells in vitro can be used for reinfusion to treat a variety of diseases, including malignant tumors, infections, and autoimmune diseases. During this process, it is necessary to perform cryopreservation and recovery operations on T cells before expansion, during expansion and after expansion...

AI Technical Summary

Problems solved by technology

The problem with this kind of cryopreservation solution is: 1) The difference between serum batches leads to unstable cryopreservation effect; 2) Contains serum components derived from xenogeneic animals (generally cattle), there is a risk of introducing xenogeneic animal pathogen infection, and cannot be used directly Clinical application; 3) The composition of serum is complex, which contains a variety of proteins, growth factors, etc., which not only fails to achieve the best cryopreservation effect for some types of cells, but may also affect the subsequent growth status of cells; 4) Not suitable for serum-free The culture system is used at the same time

[0005] The existing commercial T cell serum-free cryopreservation solutions have the following problems: 1) The formulation is complex, and one or several cryoprotectants other than DMSO need to be added; 2) Most of the human serum sources need to be added Albumin increases the complexity of ingredients, the instability of raw material sources, and the difficulty of manufacturing. It cannot achieve a true chemical composition, and there is great uncertainty in clinical use.

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