Vero cell serum-free culture medium and application thereof
A technology of serum-free culture medium and culture medium, applied in the direction of cell culture active agent, animal cells, culture process, etc., can solve the problems of increasing the cost of large-scale cell culture, unclear chemical composition, complex composition, etc.
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Embodiment 1
[0291] This embodiment provides a serum-free medium for Vero cell culture, the serum-free medium includes amino acid components, vitamin components, inorganic salt components, trace element components, carbohydrates and other molecular compound components, in,
[0292] The composition of the amino acid component is: L-arginine 45mg / L, L-aspartic acid 30mg / L, L-asparagine 95mg / L, L-cysteine 20mg / L, L-glycine 100mg / L L, L-glutamic acid 15mg / L, L-histidine 27mg / L, L-isoleucine 50mg / L, L-leucine 66mg / L, L-lysine 100mg / L, L- Methionine 8mg / L, L-phenylalanine 20m / L, L-proline 15mg / L, L-serine 50mg / L, L-threonine 20mg / L, L-tryptophan 15mg / L, L - Tyrosine 35mg / L and L-valine 30mg / L.
[0293] The composition of vitamin components is: biotin 0.1mg / L, calcium pantothenate 3mg / L, folic acid 10.33mg / L, nicotinamide 15mg / L, vitamin B6 3 mg / L, thiamine hydrochloride 5.5 mg / L, vitamin B12 1.0mg / L, riboflavin 0.9mg / L, choline chloride 35mg / L and inositol 10mg / L.
[0294] The composition ...
Embodiment 2
[0298] This embodiment provides a serum-free medium for Vero cell culture, the serum-free medium includes amino acid components, vitamin components, inorganic salt components, trace element components, carbohydrates and other molecular compound components, in,
[0299] The composition of the amino acid component is: L-arginine 100mg / L, L-aspartic acid 30mg / L, L-asparagine 55mg / L, L-cysteine 20mg / L, L-glycine 80mg / L L, L-glutamic acid 15mg / L, L-histidine 27mg / L, L-isoleucine 50mg / L, L-leucine 30mg / L, L-lysine 30mg / L, L- Methionine 30mg / L, L-phenylalanine 30m / L, L-proline 35mg / L, L-serine 50mg / L, L-threonine 20mg / L, L-tryptophan 40mg / L, L - Tyrosine 35mg / L and L-Valine 50mg / L.
[0300] The composition of vitamin components is: biotin 1.0mg / L, calcium pantothenate 8.0mg / L, folic acid 20.33mg / L, nicotinamide 10mg / L, vitamin B6 3 mg / L, thiamine hydrochloride 10 mg / L, vitamin B12 10mg / L, riboflavin 0.9mg / L, choline chloride 35mg / L and inositol 10mg / L.
[0301] The composition ...
Embodiment 3
[0305] This embodiment provides a serum-free medium for Vero cell culture, the serum-free medium includes amino acid components, vitamin components, inorganic salt components, trace element components, carbohydrates and other molecular compound components, in,
[0306] The composition of the amino acid component is: L-arginine 120mg / L, L-aspartic acid 95mg / L, L-asparagine 30mg / L, L-cysteine 15mg / L, L-glycine 80mg / L L, L-glutamic acid 15mg / L, L-histidine 27mg / L, L-isoleucine 50mg / L, L-leucine 30mg / L, L-lysine 30mg / L, L- Methionine 30mg / L, L-phenylalanine 30m / L, L-proline 15mg / L, L-serine 15mg / L, L-threonine 20mg / L, L-tryptophan 10mg / L, L - Tyrosine 10mg / L and L-Valine 10mg / L.
[0307] The composition of vitamin components is: biotin 2.0mg / L, calcium pantothenate 5.0mg / L, folic acid 10mg / L, nicotinamide 10mg / L, vitamin B6 10 mg / L, thiamine hydrochloride 5 mg / L, vitamin B12 10mg / L, riboflavin 1.0mg / L, choline chloride 100mg / L and inositol 50mg / L.
[0308] The composition of...
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