8results about How to "Avoid Biosecurity Risks" patented technology

The invention relates to the field of microbiological examination laboratory equipment, and discloses a drying and disinfecting device and method for a specimen glass slide for microbiological examination. The device comprises a machine shell, a motor and a controller, the detachable rotating shaft is in transmission connection with the driving motor; the support frame is detachably arranged on the detachable rotating shaft and forms a plurality of mounting surfaces; the glass slide mounting plate is detachably mounted on the mounting surface, and a plurality of glass slide mounting stations are arranged on the glass slide mounting plate; the drying module comprises a drying lamp; the disinfection module comprises an ultraviolet disinfection lamp; and the control module is electrically connected with the driving motor, the drying module and the disinfection module and is used for executing a drying program and a disinfection program. The problems that in an existing glass slide processing method, the drying capacity and uniformity are difficult to consider at the same time, the drying process is uncontrollable, and low efficiency and safety risks are caused by separation of the drying process and the disinfection process are effectively solved.

The invention belongs to the technical field of in-vitro transcription, and particularly discloses a 5 'UTR element with a protein expression enhancing function, an expression vector and application. The 5 'UTR is selected from any one of an SPP1d element, a TSPAN1u1 element and an SPP1d-TSPAN1u1 series element; the nucleotide sequence of the SPP1d element is as shown in SEQ ID NO: 1, the nucleotide sequence of the TSPAN1u1 element is as shown in SEQ ID NO: 2, and the nucleotide sequence of the SPP1d-TSPAN1u1 tandem element is as shown in SEQ ID NO: 3. The 5 'UTR element can significantly improve translation efficiency, has a wide enhancement effect on functional protein expression, is high in biological safety, and provides a standardized and high-performance translation regulation tool for the fields of efficient recombinant protein production, gene therapy vector optimization, vaccine research and development and the like.

The present invention discloses a genetic modification method for changing the fur color of a cat, comprising: using CRISPR / Cas9 technology to target the 17th exon of the cat KIT gene for gene editing. The present invention also provides a sgRNA specifically targeting the 17th exon of the cat KIT gene, a CRISPR / Cas9 expression vector comprising the sgRNA, and its use in changing the fur color of a cat. The present invention uses CRISPR / Cas9 technology to edit the 17th exon of the KIT gene of a civet cat, so that accurate gene modification can be achieved in the genome of the civet cat, and the fur color traits of the civet cat can be changed in a targeted manner, which can avoid the introduction of foreign cat bloodlines while changing the fur color traits in the traditional hybrid breeding process, affecting the original appearance and characteristics of the civet cat, and at the same time, no exogenous DNA sequence is introduced, effectively avoiding the biosafety risks that may be caused by traditional transgenic technology.

The utility model discloses a kind of for sample detection box sample detection box sample collector, comprising: tube body component, for loading diluent;Pipe spoon component, it can be detachably installed on tube body component, to make its sampling end stretch into tube body component;The liquid outlet channel and back gas channel that are communicated with tube body component inside are provided in the pipe spoon component, first check valve and filter component are provided in the liquid outlet channel, the first check valve is configured as only fluid from tube body component one-way flows out sample collector, second check valve is provided in the back gas channel, the second check valve is configured as only fluid from sample collector outside one-way flows into tube body component;When the sample collector is inverted, external fluid is isolated by back gas channel and the liquid in tube body component and directly reaches the bottom of tube body component, solves the prior art in the art that sample liquid is mixed with a large number of bubbles and is added into sample detection box, improves sample detection precision.

This invention discloses a microfluidic detection device based on quantum dot luminescence, comprising a microfluidic chip, including a microfluidic chip body, a sample liquid inlet, a sample chamber, a main channel, at least one branch channel, and at least one detection unit. The detection unit includes a magnetic bead chamber, a mixing channel, a first inverted U-shaped channel, a detection chamber, and a second inverted U-shaped channel arranged sequentially. An electromagnet is located on the back of the detection chamber, which is connected to a three-electrode system. A reagent chamber is located above the detection chamber, and a paraffin valve is located between the reagent chamber and the detection chamber. A heating element is located on the back of the paraffin valve. An electrochemical workstation is also included. At least one detection component includes a rotating filter and a photon counting detector, with the rotating filter facing the front of the detection chamber and the photon counting detector facing the rotating filter. This invention also discloses a microfluidic detection method. The microfluidic chip of this invention is driven entirely by gravity, eliminating the need for an external pump; it can perform simultaneous quantitative detection of multiple viruses.

The invention belongs to the technical field of biological veterinary drugs, and relates to a toxoplasma gondii TgGAP45-PLGA nano vaccine as well as a preparation method and application thereof. The vaccine is prepared by coating recombinant protein GAP45 with a coating substance, wherein the protein is derived from toxoplasma gondii slider histone; the amino acid sequence of the gene is shown in SEQ ID NO.1. After the immune protection effect of the vaccine is evaluated, it is found that the toxoplasma gondii TgGAP45-PLGA nano vaccine prepared with GAP45 as the antigen can have strong immune stimulation ability, promote activation of an immune system and remarkably reduce the infection intensity of toxoplasma gondii in a mouse body, and the toxoplasma gondii TgGAP45-PLGA nano vaccine has higher vaccine valence, is free of toxicity and is high in biological safety.

The invention belongs to the technical field of biological vaccine preparation, and particularly relates to a preparation method of a targeted antigen displayed MS2 phage VLPs nanoparticle vaccine. The preparation method sequentially comprises the following steps: preparation and activation of a targeting ligand, fixed-point transformation and expression of MS2 bacteriophage capsid protein, preparation of targeting antigen-aptamer fusion protein, folding optimization and activity pre-verification of the antigen-aptamer fusion protein, self-assembly and antigen display of targeting MS2-VLPs, purification and characterization of targeting VLPs nanoparticles, and preparation of the vaccine preparation. On the basis, by introducing the targeting ligand aiming at the specific receptor on the surface of the antigen presenting cell, accurate recognition and combination of the VLPs vaccine on the target cell are realized, the problem that the antigen display lacks targeting is fundamentally solved, the antigen delivery efficiency is remarkably improved, the off-target effect is effectively reduced, and the antigen waste is avoided.