Serum free hepatocyte medium

A medium and hepatocyte technology, applied in the field of serum-free hepatocyte medium, can solve the problems of high price, secret ingredient formula, and inability to culture without serum, and achieve the effect of good growth.
CN102559581BActive Publication Date: 2014-09-10WEST CHINA HOSPITAL SICHUAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
WEST CHINA HOSPITAL SICHUAN UNIV
Publication Date
2014-09-10

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Abstract

The invention provides a novel serum free medium and a preparation method and application of the medium. The serum free medium overcomes defects of the traditional serum media, and is obviously superior to serum free media reported in the conventional documents, improves the possibility of supporting systematical treatment clinical application through cell transplantation, tissue engineering liver and bioartificial liver, and has high practical value.
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Description

technical field

[0001] The invention relates to a hepatocyte culture medium, in particular to a serum-free hepatocyte culture medium. Background technique

[0002] Liver parenchymal cells, namely hepatocytes, are the main executors of liver function, and their total weight accounts for about 80% of liver weight. Hepatocytes absorb and process nutrients and other substances in the blood, such as glucosylating bilirubin and finally forming bile to be secreted into the intestinal tract. At the same time, hepatocytes also synthesize albumin, complement proteins, glycogen (glucose storage) and coagulation In addition, liver cells also convert ammonia in the blood into urea to complete detoxification. The liver also plays an important role in the regulation of carbohydrates, lipids and amino acids. The specific functions of the liver are completed by various liver-specific enzymes synthesized by liver cells, which synthesize and store the nutrients absorbed by the gastrointestina...

Claims

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