A method for inducing neural stem cells and its application

A technology of neural stem cells and neural precursor cells, applied in the fields of neural stem cells and biomedicine, can solve the problems of large trauma of fibroblasts, difficulty in culturing fibroblasts, and easy pollution of cell sources.
CN110396499BActive Publication Date: 2021-06-15WISEHEART MEDICAL VALLEY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
WISEHEART MEDICAL VALLEY CO LTD
Publication Date
2021-06-15

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Abstract

The invention discloses a method and composition for inducing neural stem cells, which can induce peripheral blood mononuclear cells to form neural stem cells. The neural stem cells can express genes related to neural stem cells, and can differentiate into neurons, astrocytes and oligodendrocytes. The dopaminergic neural precursor cells formed by the in vitro induction and differentiation of the above-mentioned neural stem cells can be transplanted into the striatum of the PD mouse model without tumor formation, which can improve the behavior of the mouse Parkinson's disease model and delay the progression of Parkinson's disease. The neural stem cell induction and differentiation method provided by the present invention is simple and quick to operate, less invasive, and has good safety, and is expected to be used in the treatment of Parkinson's disease.
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Description

technical field

[0001] The present invention relates to the field of biomedicine and the technical field of neural stem cells, in particular to a method for inducing neural stem cells using a non-integrated, temperature-inactivating Sendai virus vector and the use of dopaminergic neural precursor cells of directed differentiation in Parkinson's disease cell transplantation therapy the use of. Background technique

[0002] Stem cell transplantation brings hope for the treatment of many neurological diseases, such as Parkinson's disease, amyotrophic lateral sclerosis, brain and spinal cord trauma, etc. Studies have shown that the use of neural stem cells derived from embryonic stem cells has certain effects in the treatment of the above diseases, but the tumorigenicity of embryonic stem cells, immune rejection of allogeneic transplantation, and ethical disputes limit its application. Induced pluripotent stem cells (iPSCs) were obtained from human fibroblasts and differentiate...

Claims

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