The invention discloses a high-yield and disease-resistant planting method for lentinula edodes. The method comprises the following steps that firstly, materials are prepared, wherein a lentinula edodes culture medium is prepared from dried poultry litter, bamboo leaf residues, Chinese chestnut peel, wheat bran, soybean straw, corn stigma, dried silkworm manure, saw powder, antibacterial sustained-release filler and a sterilization additive; secondly, high-temperature sterilization is conducted, wherein the lentinula edodes culture medium mixed evenly in the first step is subjected to high-temperature sterilization; thirdly, inoculation is conducted, wherein the lentinula edodes culture medium obtained after high-temperature sterilization is inoculated with solid lentinula edodes strain inoculating sticks, stirring is conducted after sealing, and a culture material is obtained; fourthly, bagging is conducted, wherein the lentinula edodes culture medium inoculated in an aseptic environment is bagged, air holes are formed, spawn running is conducted, after culture, a bag is filled with mycelia, and then conventional culture is conducted. By means of the planting method for the lentinula edodes, the yield and the quality of the lentinula edodes can be effectively improved.