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Device for controlling oxygen concentration in magnetotactic bacterium culturing process and culture method

A technology for magnetotactic bacteria and oxygen concentration, which is applied to the device for controlling oxygen concentration in the growth environment of microaerophilic magnetotactic bacteria and the field of devices for controlling oxygen concentration during the cultivation of magnetotactic bacteria, which can solve problems affecting the synthesis of magnetosomes, etc. Achieve the effect of reducing environmental requirements, low cost, and eliminating pollution problems

Pending Publication Date: 2019-07-19
GANSU UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the oxygen concentration in its growth environment cannot be precisely controlled, it will directly affect the synthesis of magnetosomes.

Method used

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  • Device for controlling oxygen concentration in magnetotactic bacterium culturing process and culture method
  • Device for controlling oxygen concentration in magnetotactic bacterium culturing process and culture method
  • Device for controlling oxygen concentration in magnetotactic bacterium culturing process and culture method

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Embodiment 1

[0049] The method for controlling oxygen concentration in the growth environment of magnetotactic bacteria comprises the following steps:

[0050] Step A: Device design and installation; specific components include: nitrogen tank, workbench, laboratory square seat bracket (with parallel clamps), rubber air tube (long), 5cm air tube (several), 20cm air tube (several ), bracket, three-way tube (several), right-angle elbow, 1mL volume disposable syringe (keep the needle, remove the plunger, fill with cotton, several), 10cm needle extension tube (several), vial (diameter 52mm, height 95mm, total volume 100mL, mouth diameter 20mm, flat bottom), 20mm butyl rubber stopper, aluminum-plastic bottle cap of the same specification, and capping pliers of the same specification.

[0051] installation method:

[0052] ①Connection of the rubber air pipe: one end of the rubber air pipe (long) is connected to the gas outlet of the nitrogen tank, and the other end is connected to the horizontal...

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Abstract

The invention relates to a device for controlling the oxygen concentration in the magnetotactic bacterium culturing process and a culture method, and belongs to the technical field of control of magnetotactic bacterium culturing condition. The device comprises a gas source (1), a penicillin bottle (2) and a gas passing pipe (3), wherein the gas source (1) comprises a nitrogen source and an oxygensource; the gas inlet of the gas passing pipe (3) is connected with the gas source (1); the gas outlet of the gas passing pipe is connected with an injector (4) without a piston, and a syringe needleof the injector (4) without a piston is connected with the penicillin bottle (2); and the injector (4) without a piston is filled with cotton (5). The device is simple and easy to operate, can meet the micro-aerobic requirement, ensures that bacteria in the operating process cannot be easily polluted, is low in cost, and is simple and feasible.

Description

technical field [0001] The present invention relates to the technical field of control of magnetotactic bacteria culture conditions, in particular to a device for controlling the oxygen concentration in the magnetotactic bacteria culture process and a method for culturing magnetotactic bacteria, that is, a device and method for controlling the oxygen concentration in the growth environment of microaerophilic magnetotactic bacteria . Background technique [0002] Magnetotactic bacteria are a group of bacteria that can generate magnetosome nanoparticles in vivo through biomineralization. Due to the unique properties of magnetosomes, the current research on magnetosomes has become a research hotspot in many related fields, and its development and application have potential huge scientific research value and commercial value. However, magnetotactic bacteria are a type of microaerophilic bacteria that have strict requirements on the growth environment, which are different from t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/24C12M1/04C12N1/20
CPCC12M23/08C12M29/06C12N1/20
Inventor 张薇薇
Owner GANSU UNIV OF CHINESE MEDICINE
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