Artificial mouth simulating device

Abstract

The invention discloses an artificial mouth simulating device, comprising a culture medium tank, multi-channel pumps, flow chambers, drug sample solution bottles and a waste solution collection tank, wherein the culture medium tank is connected with one multi-channel pump by pipelines; the multi-channel pump is connected with the flow chambers by pipelines; Luer connectors are arranged on the pipelines between the multi-channel pump and the flow chambers and are connected with the drug sample solution bottles by pipelines; the flow chambers and the waste solution collection tank are connectedby pipelines; and the other multi-channel pump is arranged on the connection pipelines between the flow chambers and the waste solution collection tank. The device has the beneficial effects of simple structure, low cost, short cycle of experiment, good repeatability of the experiment result, high precision control and low possibility of being polluted.

Description

technical field [0001] The invention relates to a simulation device for carrying out experimental research in a simulated oral cavity environment, in particular to an artificial oral cavity simulation device. Background technique [0002] For a long time, people usually conduct bacteriological research in the form of pure culture of microorganisms. This kind of research greatly simplifies the influence of the external environment, helps people understand the habits of many individual microorganisms, and has achieved many important research results. Made great contributions to the development of medicine and microbiology. However, most bacteria in nature do not exist in the free state under pure culture, but in the form of biofilm. Biofilm is a special cell group structure formed by a large number of microorganisms gathered on the surface of a certain medium and wrapped in exopolysaccharides secreted by itself. It can be composed of a single type of bacteria, but more often ...

AI Technical Summary

Problems solved by technology

The advantages and disadvantages of this model are compared with the "chemostat-flow chamber model: the improvement of this model is that the temperature of the constant flow culture chamber for biofilm growth is controllable, but the disadvantages of the "chemostat-flow chamber model" are There is not much improvement in this model, and this model still has the following defects: 1) The structure of the whole system is still quite complicated, and there are many feeding systems, pipelines and pumps; Difficulty of pollution control; 2) long experiment period

The biggest advantage of this model is that the design of the device is simple, the cost is low, and it is more convenient to operate; but this model has the following defects: 1) its biofilm culture mode is static culture, which cannot simulate the flow of saliva in the human mouth; 2) Anaerobic culture and aerobic operation also have a certain impact on the development of biofilm; 3) Its medium is more complicated to prepare

The design of the model is very delicate, and it can realize some high-precision control and monitoring of the growth environment and process of the biofilm, and can independently control the growth of multiple biofilm samples; but the model has the following defects: 1) Because of the model The control precision is high, so its construction cost is high; 2) The experimental cycle of the model is relatively long, and an experiment may take several weeks

3) The model plaque needs to grow in an anaerobic environment, which is different from the actual in vivo biofilm growth environment

But it still has the following defects: 1) the temperature of the reactor is not controlled; 2) the experimental period is long; 3) the repeatability of the experimental results is poor

[0010] In summary, the existing in vitro models of oral biofilms have certain shortcomings and cannot fully meet the needs of research.

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