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Penicillium chrysogenum with high salt and alkali resistance as well as separation method and application thereof

A technology of Penicillium chrysogenum and separation method is applied in the field of high salt-alkali-resistant Penicillium chrysogenum and its separation, and achieves high impermeability, good effect, and the effect of improving degradability.

Active Publication Date: 2019-12-13
内蒙古现代农牧研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]Using microorganisms to degrade straw and return it to the field is an important measure to improve soil, especially saline-alkali soil. Soil can play a very good role, but in saline-alkali soil, due to the high concentration of salt and high pH environment, these strains are difficult to play their due role. In order to efficiently degrade straw in a short period of time, To realize fertilizer returning to the field, it is very necessary to screen highly saline-alkali-resistant fungi from saline-alkali soils. Therefore, the present invention proposes a high-saline-alkali-resistant Penicillium chrysogenum and its separation method and application to solve the problems in the prior art. inadequacies of

Method used

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  • Penicillium chrysogenum with high salt and alkali resistance as well as separation method and application thereof
  • Penicillium chrysogenum with high salt and alkali resistance as well as separation method and application thereof
  • Penicillium chrysogenum with high salt and alkali resistance as well as separation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0025] A method for isolating highly saline-alkali-resistant Penicillium chrysogenum, comprising the following steps:

[0026] Step 1: On the solid plate of 250.0g / L corn stalk powder medium containing different concentrations of NaCl, coat the suspension of straw powder through gradient dilution. The corn stalk powder medium consists of 10g corn stalk powder, 4g (NH 4 ) 2 SO 4 , 0.5gMgSO 4 ·7H 2 O, 2gKH 2 PO 4 , 1g peptone, 16g agar and 1000mL deionized water, the pH value of the rice straw powder medium is 6.5, and the straw powder suspension is 5cm thick. The straw powder suspension was obtained after the clear liquid, and the treatment on each corn straw powder medium solid plate was repeated 3 times, and then after 4 days of cultivation, a single colony with good growth was picked and preserved for later use;

[0027] Step 2: inoculate the single bacterium colony that is picked and preserved in step 1 on the screening medium CMC-Na solid medium until a transparent r...

Embodiment 2

[0031] according to figure 1 , 2 As shown, the present embodiment proposes the morphological identification of the bacterial strain of transparent circle:

[0032] Inoculate the screened transparent ring-shaped bacterial strains on the corn stalk powder medium containing 5% NaCL, cultivate them at 30°C for 5 days, and then observe the colony morphology. Insert the cover slip obliquely into the middle of the 5% NaCl corn stalk powder medium, inoculate a 7mm diameter bacterial strain in the shape of a transparent ring (the mycelium of the strain faces downward) directly in front of the cover slip, and then place the plate In a constant temperature incubator at 30°C, observe the growth of mycelia regularly. After the mycelium climbs up the cover glass, cut off the medium connected to the cover glass, take out the cover glass, and observe the mycelia and spores under a microscope Morphology, after culturing at 30°C for 9 days on corn stalk powder medium containing 5% NaCl, the d...

Embodiment 3

[0035] The present embodiment proposes the high salt resistance of Penicillium chrysogenum W2 and the identification of the optimum salt concentration: the corn stalk powder medium (PH is 7.0) with gradient NaCl content is prepared, and the salt concentration (mass fraction) is increased from 0% to 31 % (saturation concentration), each level increased by 2%, an equal amount of strains were inoculated in the center of the medium, and cultivated at 30°C for 1 week. In the culture medium, all strains can grow, but with the increase of salt concentration, the growth of the strain is obviously inhibited, and the growth is relatively slow at the concentration of 1%-5%; The growth of the strain slows down sharply at the concentration of %, and the bacteria basically does not grow at the concentration of 27%-31%. Therefore, the strain has the characteristics of salt resistance and belongs to the fungus with high salt resistance characteristics;

[0036]Use the series of salt gradient ...

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Abstract

The invention discloses penicillium chrysogenum with high salt and alkali resistance as well as a separation method and an application thereof. The method comprises the following steps: coating a cornstraw powder culture medium solid flat plate containing 250.0 g / L NaCl with different concentrations with a straw powder suspension subjected to gradient dilution, treating and repeating for 3 times,culturing for 4-15 days, picking well-grown single colonies, inoculating the single colonies onto a screening culture medium CMC-Na solid culture medium, and generating a transparent ring-shaped strain to obtain penicillium chrysogenum with a preservation name of penicillium chrysogenum W2 and a preservation number of CGMCC NO. 17972. The penicillium chrysogenum with high saline-alkali resistancehas high impermeability, is beneficial to improving the degradation capacity of straws in a high saline-alkali environment, can also be directly used for decomposing straw fermentation and other biological substances in fields, and has good effects on improvement of secondary salinization or continuous cropping obstacle soil and environmental pollution treatment.

Description

technical field [0001] The invention relates to the field of microbiology engineering and technology, in particular to a highly saline-alkali-resistant Penicillium chrysogenum and its isolation method and application. Background technique [0002] my country is rich in straw resources and has a wide range of sources, ranking first in the world. Especially since the agricultural reform, with the substantial increase in grain production, the output of crop straws has also increased rapidly, and has become an important source of straw resources in my country. With the continuous development of science and technology, the field of straw utilization has also been greatly expanded, such as straw can be used as fuel, feed, base material (substrate for raising seedlings), industrial raw materials and so on. However, at present, the comprehensive utilization rate of crop straw resources is still low, and most of them are extensively treated, or even burned directly in the field, whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02B09C1/10C05F11/08C12R1/82
CPCC12N1/02B09C1/105C05F11/08C12R2001/82C12N1/145Y02W30/40
Inventor 张森林陶润志冯泽娟
Owner 内蒙古现代农牧研究院
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