6 results about "Cover slip" patented technology

An apparatus for rapid screening of tumor stem cells and a method. The apparatus comprises a microscope slide (1) and a cover slip (2) for use under a microscope; the microscope slide (1) is located at the bottom and the cover slip (2) is located at the top; the microscope slide (1) and the cover slip (2) are sealed around their periphery and form an included angle of 2-10 degrees therebetween, the distance between front ends is 200-300 microns, the distance between rear ends is 100-150 microns, a liquid inlet tube (4) and a cleaning tube (5) are provided at the front ends, and a liquid outlet tube (6) is provided at the rear ends; the liquid inlet tube (4) is connected to a blood test vial (7), the cleaning tube (5) is connected to a cleaning vial or a reagent vial (8), and the liquid outlet tube (6) is connected to a blood collection vial (9); the microscope slide (1) is provided with one or more U-shaped grooves (11), the depth of the U-shaped groove (11) is 100-500 microns, and the width of the U-shaped groove (11) is 100-300 microns.

This invention relates to the field of microscope coverslip technology, specifically to a coverslip holder, comprising an L-shaped inner gripper and an L-shaped outer gripper. The inner gripper overlaps and is disposed inside the outer gripper. Both the inner and outer grippers include a vertically connected clamping head and a handle. The tail end of the handle of the inner gripper is fixedly connected to the tail end of the handle of the outer gripper, forming a clamping space between the clamping heads of the inner and outer grippers. The handle of the inner gripper includes a fixed handle and a movable handle, with a spring connecting the fixed handle and the movable handle. This invention solves the problem in existing technologies where samples easily spill from the coverslip when it is clamped onto or removed from a heating plate.

A slide-like hanging-drop cell culture device (100) is disclosed for generating, maintaining, observing, and processing three-dimensional tissues. The device provides an array of culture cavities (102) with concave bottoms (103) configured to promote aggregation and, after re-inversion, to seat formed tissues for repeatable positioning. An underside imaging cavity (104) with an inner step (105) accommodates an immersion medium (162) to enable high-magnification observation from below, while the upper face supports coverslip-based imaging using a coverslip (160), thereby permitting bidirectional microscopy without transfer. A detachable cover (120) forms a closed arrangement and incorporates hydration features (124, 126) to reduce droplet evaporation during incubation. Optional accessories include a wash array (140) arranged to deliver fluids via conduits (142) and a wash-filter array (150) with conduits (152), extensions (154) and an internal filter (155) to retain fragments during processing. Methods of use include dispensing cell suspensions, forming hanging-drops, incubating, re-inverting for positioning, washing or permeabilizing on the slide, imaging from either side, and retrieving (182) or embedding (170) tissues for downstream analysis with optional lift- out using forceps (180).

This invention discloses a non-oil immersion large numerical aperture microscope objective, composed of a first lens group S1, a second lens group S2, a third lens group S3, and a fourth lens group S4. The total length (Lob) of the four lens groups is ≤50mm, and the focal length (EFL) of the microscope objective is... ob The focal length EFL of the first lens group S1 S1 The focal length EFL of the second lens group S2 S2 The focal length EFL of the third lens group S3 S3 The focal length EFL of the fourth lens group S4 S4 Satisfying: -3.40 ≤ EFL S1 / EFL ob ≤-3.90, 5.0≤EFL S2 / EFL ob ≤9.0, 8.0≤EFL S3 / EFL ob ≤21.0, 1.30≤EFL S4 / EFLob≤1.75, HI / EFLob≤0.139, where HI is the height of the observed object. The second lens group S2 can move along the optical axis. Its advantage is that the numerical aperture of the objective lens can be ≥0.95 without oil immersion. By adjusting the distance between the objective lens elements, the imaging blur caused by the processing error of the coverslip can be corrected, and the difference caused by the thickness of the coverslip can be compensated. It has a good effect on correcting chromatic aberration without using special optical materials such as DOE.

This invention discloses a slide observation stage for pathological anatomy, belonging to the field of medical equipment. It includes a support component comprising a base detachably connected to the arm of a microscope, a support seat slidably mounted vertically on the base, and a support platform slidably connected horizontally to the top of the support seat. Before observing the sealed pathological slides using this observation stage, the top of the coverslip is wiped clean with alcohol. This effectively removes dust adhering to the surface of the coverslip and simultaneously sterilizes bacteria on the surface. This effectively prevents dust and bacteria from the ambient air from adhering to the surface of the coverslip during the examination process, thus avoiding obstruction of the pathological tissue beneath the coverslip during subsequent observation and facilitating the observation of the pathological slides.

This invention relates to the field of medical device technology and discloses a fully automated fluorescence staining microscopic image scanning and analysis system, including a fully automated fluorescence staining imager. A coverslip compartment is fixedly connected to the top outer wall of the fully automated fluorescence staining imager for storing coverslips. A cover-applying module is provided on the top of the fully automated fluorescence staining imager. Utilizing the curvature formed by the coverslip during slide removal, combined with a rotating pressing action, when the curved coverslip moves above the slide and presses down, the lowest point of its curved surface preferentially forms point contact with the sample to be tested. As the rotating frame continues to rotate, the coverslip is gradually pressed and flattened from the contact point to both sides, allowing the sample to be tested to be evenly spread radially outward from the center, effectively shortening the air escape path and avoiding air bubble residue. Simultaneously, the internal elastic potential energy of the coverslip is released smoothly, preventing the coverslip from breaking due to localized stress concentration, thereby improving the sealing quality.