15 results about "Cover slip" patented technology

The present invention relates to the field of optical imaging technology, and discloses a microscope phase contrast objective lens, comprising a first lens group with positive refractive power, a second lens group with positive refractive power, an object plane and a cover glass, wherein the first lens group is located below the second lens group, and the absolute value of the refractive power of the first lens group is greater than the absolute value of the refractive power of the second lens group. The present invention is arranged by setting the first lens group and the second lens group in the phase contrast objective optical system, and by limiting the focal length, refractive index, Abbe number and thickness of the first lens group and the second lens group, so that the field curvature, distortion and aberration sensitivity of the phase contrast objective optical system are further improved, thereby ensuring the optical performance of the phase contrast objective optical system, so that the objective optical system has the characteristics of small magnification, large numerical aperture, high resolution performance, small chromatic aberration under a large field of view, small number of lenses, and short total length of the objective optical system.

The utility model relates to the field of microscopes, in particular to a microscope and a microscope objective. The microscope objective comprises a first lens, a second lens, a third lens and a fourth lens which are sequentially and coaxially arranged from the image space to the object space. The first lens is a negative lens, a second lens, a third lens and a fourth lens; the focal length of the microscope objective is f, and the focal lengths of the first lens, the second lens, the third lens and the fourth lens are f1, f2, f3 and f4 in sequence; wherein the result is-3.0 lt; f1 / flt; -2.5,-2.5; 2.0 lt; f2 / flt; 2.8); 101t; 101t; f3 / flt; 50; 1.2 1t; f4 / flt; and 1.5. The microscope objective can meet the requirements of low conjugate distance, low processing cost and long working distance while obtaining high-resolution imaging performance, and is suitable for being matched with a thick cover glass to complete automatic biological cell detection.

The utility model discloses a microbiological detection board card, which is characterized by comprising a glass slide, the cover glass is arranged opposite to the glass slide in the thickness direction of the glass slide; the supporting layer is provided with one or more hollow patterns, the supporting layer is located between the glass slide and the cover glass, and the supporting layer, the glass slide and the cover glass are connected to form one or more independent cavities; the chamber includes an observation chamber. Seepage is reduced through fragmented synchronous bonding, and convenience of detection of different microorganisms in different environments is improved.

The present invention addresses the problem of providing a cover slip sticking device whereby the presence / absence of an immersion tank or the state of an immersion tank can be confirmed. As a solution to this problem, the present invention comprises an immersion tank (32) in which a clearing agent is retained and which stores a basket (21) in which are stored a plurality of slide glasses (22) to which a specimen sample is affixed, an immersion tank arrangement part (33) in which the immersion tank (32) is arranged, an imaging device (70) provided to the immersion tank arrangement part (33), and reporting units (74, 76) for reporting the presence / absence of the immersion tank (32) and the state of the immersion tank (32) in the immersion tank arrangement part (33) as imaged by the imaging device (70).

The present invention relates to the field of cell detection technology, and in particular to a method for precise axial positioning and judgment of cell microscopic Raman detection. It includes: selecting glass slides with different Raman spectra as slides and cover slides; obtaining normalized standard Raman spectra through testing; fixing the cell sample to be tested between two glass slides, placing it under a microscope objective, adjusting the focal length under ordinary white light illumination, and then switching to laser to form a focused light spot that excites the Raman signal on the cell sample, and detecting the Raman spectrum of the sample at this time; constructing a related linear equation group, using a regression algorithm to solve the intensity coefficient of the Raman spectra of the two glass slides, and calculating the cell Raman spectrum; constructing a normalized positioning coefficient of the light spot axial direction according to the intensity coefficient of the Raman spectra of the two glass slides; constructing and training a discrimination model; and using the trained discrimination model to discriminate the cell sample to be tested. The advantage is that no labeling, staining, etc. are required, and the objective positioning and judgment of the cell Raman axial excitation position can be directly achieved.

An apparatus for rapid screening of tumor stem cells and a method. The apparatus comprises a microscope slide (1) and a cover slip (2) for use under a microscope; the microscope slide (1) is located at the bottom and the cover slip (2) is located at the top; the microscope slide (1) and the cover slip (2) are sealed around their periphery and form an included angle of 2-10 degrees therebetween, the distance between front ends is 200-300 microns, the distance between rear ends is 100-150 microns, a liquid inlet tube (4) and a cleaning tube (5) are provided at the front ends, and a liquid outlet tube (6) is provided at the rear ends; the liquid inlet tube (4) is connected to a blood test vial (7), the cleaning tube (5) is connected to a cleaning vial or a reagent vial (8), and the liquid outlet tube (6) is connected to a blood collection vial (9); the microscope slide (1) is provided with one or more U-shaped grooves (11), the depth of the U-shaped groove (11) is 100-500 microns, and the width of the U-shaped groove (11) is 100-300 microns.

This invention relates to the field of microscope coverslip technology, specifically to a coverslip holder, comprising an L-shaped inner gripper and an L-shaped outer gripper. The inner gripper overlaps and is disposed inside the outer gripper. Both the inner and outer grippers include a vertically connected clamping head and a handle. The tail end of the handle of the inner gripper is fixedly connected to the tail end of the handle of the outer gripper, forming a clamping space between the clamping heads of the inner and outer grippers. The handle of the inner gripper includes a fixed handle and a movable handle, with a spring connecting the fixed handle and the movable handle. This invention solves the problem in existing technologies where samples easily spill from the coverslip when it is clamped onto or removed from a heating plate.

A slide-like hanging-drop cell culture device (100) is disclosed for generating, maintaining, observing, and processing three-dimensional tissues. The device provides an array of culture cavities (102) with concave bottoms (103) configured to promote aggregation and, after re-inversion, to seat formed tissues for repeatable positioning. An underside imaging cavity (104) with an inner step (105) accommodates an immersion medium (162) to enable high-magnification observation from below, while the upper face supports coverslip-based imaging using a coverslip (160), thereby permitting bidirectional microscopy without transfer. A detachable cover (120) forms a closed arrangement and incorporates hydration features (124, 126) to reduce droplet evaporation during incubation. Optional accessories include a wash array (140) arranged to deliver fluids via conduits (142) and a wash-filter array (150) with conduits (152), extensions (154) and an internal filter (155) to retain fragments during processing. Methods of use include dispensing cell suspensions, forming hanging-drops, incubating, re-inverting for positioning, washing or permeabilizing on the slide, imaging from either side, and retrieving (182) or embedding (170) tissues for downstream analysis with optional lift- out using forceps (180).

This invention discloses a non-oil immersion large numerical aperture microscope objective, composed of a first lens group S1, a second lens group S2, a third lens group S3, and a fourth lens group S4. The total length (Lob) of the four lens groups is ≤50mm, and the focal length (EFL) of the microscope objective is... ob The focal length EFL of the first lens group S1 S1 The focal length EFL of the second lens group S2 S2 The focal length EFL of the third lens group S3 S3 The focal length EFL of the fourth lens group S4 S4 Satisfying: -3.40 ≤ EFL S1 / EFL ob ≤-3.90, 5.0≤EFL S2 / EFL ob ≤9.0, 8.0≤EFL S3 / EFL ob ≤21.0, 1.30≤EFL S4 / EFLob≤1.75, HI / EFLob≤0.139, where HI is the height of the observed object. The second lens group S2 can move along the optical axis. Its advantage is that the numerical aperture of the objective lens can be ≥0.95 without oil immersion. By adjusting the distance between the objective lens elements, the imaging blur caused by the processing error of the coverslip can be corrected, and the difference caused by the thickness of the coverslip can be compensated. It has a good effect on correcting chromatic aberration without using special optical materials such as DOE.

This invention discloses a slide observation stage for pathological anatomy, belonging to the field of medical equipment. It includes a support component comprising a base detachably connected to the arm of a microscope, a support seat slidably mounted vertically on the base, and a support platform slidably connected horizontally to the top of the support seat. Before observing the sealed pathological slides using this observation stage, the top of the coverslip is wiped clean with alcohol. This effectively removes dust adhering to the surface of the coverslip and simultaneously sterilizes bacteria on the surface. This effectively prevents dust and bacteria from the ambient air from adhering to the surface of the coverslip during the examination process, thus avoiding obstruction of the pathological tissue beneath the coverslip during subsequent observation and facilitating the observation of the pathological slides.

An apparatus is proposed for identifying respective cover slip regions of respective cover slips having respective tissue sections on a specimen slide, which has multiple optical identifiers. The apparatus includes a planar light source, an image acquisition unit, a holding unit for positioning the specimen slide between the planar light source and the image acquisition unit, a slit diaphragm, which has multiple opening slits, reversibly positionable between the planar light source and the specimen slide, and an illumination unit, which is designed to illuminate that surface of the specimen slide which faces toward the image acquisition unit. Furthermore, the apparatus includes a monitoring unit which is designed, on the basis of a completely illuminated transmitted light image, an incident light image, and a partially darkened transmitted light image, to assign respective tissue sections to respective optical identifiers.

This IF Moisture Chamber and Workstation related processes result in an optimal environment that reduces risk of poor quality IF staining & processing outcomes. This workstation results in less antibody consumption and end to end processing costs reduction for standard IF staining protocols. The universal dish and imbedded devices are multipurpose and can be reused to simultaneously process up to six small circular 12 millimeter diameter coverslips or two standard larger sized circular or square coverslips This workstation eliminates the need to use a forceps to precisely grasp, hold, move and tilt each individual coverslip multiple times for fluid removal during a series of repetitive staining and dumping activities typically required for full processing of each coverslip. Consequently this device facilitates exceeding storage and processing standards correlated with high quality coverslips' specimens staining for subsequent mounted slides placed under microscopic examination and image capture of specimen cell structures and compositions.

This invention relates to the field of medical device technology and discloses a fully automated fluorescence staining microscopic image scanning and analysis system, including a fully automated fluorescence staining imager. A coverslip compartment is fixedly connected to the top outer wall of the fully automated fluorescence staining imager for storing coverslips. A cover-applying module is provided on the top of the fully automated fluorescence staining imager. Utilizing the curvature formed by the coverslip during slide removal, combined with a rotating pressing action, when the curved coverslip moves above the slide and presses down, the lowest point of its curved surface preferentially forms point contact with the sample to be tested. As the rotating frame continues to rotate, the coverslip is gradually pressed and flattened from the contact point to both sides, allowing the sample to be tested to be evenly spread radially outward from the center, effectively shortening the air escape path and avoiding air bubble residue. Simultaneously, the internal elastic potential energy of the coverslip is released smoothly, preventing the coverslip from breaking due to localized stress concentration, thereby improving the sealing quality.

The present invention relates to the field of optical technology, and specifically to an immersion microscope objective lens, wherein a cover glass is arranged on one side of the object surface, and a first lens group, a second lens group and a third lens group are arranged in sequence on one side of the cover glass, and a liquid is arranged between the cover glass and the first lens group. The present invention arranges the first lens group, the second lens group and the third lens group in the immersion microscope objective lens optical system, wherein the first lens group has positive refractive power, the second lens group has positive refractive power, and the third lens group has negative refractive power, so that the entire immersion microscope objective lens optical system has good optical performance, and by limiting the focal distance, refractive index, Abbe number and thickness of the first lens group and the second lens group, the field curvature, distortion and aberration sensitivity of the objective lens optical system are further improved, thereby ensuring the optical performance of the immersion microscope objective lens optical system.

The invention discloses a fluorescent microscopic observation method for the attachment condition of hibiscus pollen on stigmas, which comprises the following steps: firstly, selecting hibiscus flowers which bloom completely, picking off pistils, and quickly cutting stigmas to obtain stigma samples; putting the column head sample into the stationary liquid, and standing for more than 24 hours; taking out the column head sample from the stationary liquid, longitudinally cutting from the top end of the column head, and averagely dividing the column head into a plurality of parts to obtain a column head tissue; the stigma tissue is soaked in a sodium hydroxide solution for standing, and the standing time does not exceed 3 hours; taking a column head sample, washing with distilled water, and dyeing with a water-soluble aniline blue solution; placing the stigma sample in a glass slide, and gently rolling the stigma sample into a slice by using a cover glass, so that the tissue is semitransparent; and placing the glass slide on which the column head sample is placed under a fluorescence microscope for observation. The method provided by the invention can effectively observe the attachment condition of the hibiscus pollen on the stigma, and provides support for evaluation of the stigma pollen attachment condition and research of hibiscus pollination biology.