Device and method for analyzing and counting blood cells by lensless holographic diffraction imaging

Abstract

The invention discloses a device and method for analyzing and counting blood cells by lensless holographic diffraction imaging, relating to blood cell detection and counting technology. The invention has a lensless simple structure and wide view, can realize miniaturization, and can monitor thousands of cells with different concentrations in real time. The device comprises a glass slide for placing a sample and a cover slip arranged on the glass slide, and is characterized in that a CCD or CMOS chip is arranged below the glass slide, a light source is arranged above the glass slide, an aperture is arranged between the light source and the glass slide and is arranged on the light extraction route of the light source. The method comprises the following steps: putting a blood cell sample between the glass slide and the cover slip and with well pressing, adjusting the light source above the glass slide, and adjusting the position of the sensitive chip below the glass slide to let the light emitted from the light source pass through the aperture and be vertically incident on the cover slip, and letting the CCD or CMOS ship arranged below the glass slide record the interference images after the light passes through the blood cell sample.

Description

[0001] technical field [0002] The invention relates to a blood cell detection and counting technology, including a blood cell detection and counting device and method. Background technique [0003] There is a huge demand for simple, low-cost, single-use diagnostic tools for HIV detection in developing countries. The number of T lymphocyte subsets CD4+ per milliliter of blood is less than 200 and is clinically diagnosed as AID. This technology faces great challenges, which require simultaneous monitoring or counting of thousands of micro-targets. Enumerating CD4 lymphocytes from whole blood using point-of-care technology faces 2 main problems: acquiring cells in whole blood with high throughput, and rapidly enumerating the acquired cells. [0004] Commonly used large-scale fluidic methods require pretreatment, and the fluorescent labeling process is time-consuming. In addition, the counting process uses a fluorescent microscope to acquire images at multiple positions in t...

AI Technical Summary

Problems solved by technology

Enumerating CD4 lymphocytes from whole blood using point-of-care technology faces 2 main problems: acquiring cells in whole blood with high throughput and rapidly enumerating the acquired cells

[0005] Commonly used label-free CD4 cell monitoring technology uses surface chemical technology to capture cells in microfluidic channels, and its counting is still performed under a conventional microscope, which is time-consuming

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