Space transcriptome database building and sequencing method and device adopted for same

Abstract

The invention discloses a space transcriptome sequencing technology device based on tissue and chip space position information. The device comprises a cover slip and a silicon-based oligonucleotide chip, wherein an anchoring probe is arranged on the silicon-based oligonucleotide chip; a gap between the cover slip and the silicon-based oligonucleotide chip is used for accommodating a tissue freezing section with the thickness of 10-50 um. Meanwhile, the invention further provides a space transcriptome sequencing technology method adopting the device. According to the device and the method, sufficient RNA (ribonucleic acid) samples can be effectively acquired from a small quantity of cells, uniform or overall characterization of a sequencing result is avoided, further, difference of different phenotypes of cell transcript information in tissue can be distinguished according to cell heterogeneity, and transcript information with low abundance is mined, so that that more accurate transcript information with high specificity is provided for fields of biology, medicine and agriculture.

Description

technical field [0001] The invention relates to the fields of biology, medicine, agronomy and the like, in particular to a space transcription library sequencing technology for obtaining tissue cell space position information by using chip probes. This technology can accurately and reproducibly label and classify cells according to their location, category, and state information. It can isolate RNA from living cells in natural tissue microenvironments while retaining the location distribution in cell tissues. Through processes such as high-throughput sequencing and bioinformatics analysis, the complete expression profiles of tissue cells in different spatial locations can be quantitatively obtained at one time. Background technique [0002] Transcriptome is a collection of all RNAs transcribed from a specific tissue or cell at a certain developmental stage or functional state. Transcriptome sequencing is the sequencing of all mRNAs that can be transcribed in a specific cell...

AI Technical Summary

Problems solved by technology

With such tiny quantities of molecules, any degradation, sample loss, or contamination can have a serious impact on sequencing quality

Moreover, multiple amplifications are prone to experimental errors, such as uneven coverage of the genome or transcriptome, high operating error rate, poor repeatability, background noise, and inaccurate quantification, all of which make single-cell sequencing difficult to be widely used and promoted.

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