20 results about "Molecular pathology" patented technology

The invention discloses a psychological health monitoring and self-adaptive nursing decision-making system and method based on electroencephalogram signals, and relates to the field, and the method comprises the following steps: S1, collecting electroencephalogram signals and eye movement characteristics, and generating a multi-modal original signal data set; s2, electroencephalogram alpha wave asymmetry and eye movement characteristics are extracted, and delta-alpha spectrum overlapping pathological interference is eliminated; s3, inputting an evaluation model and a rule base, matching a DLB pathological marker and a symptom association rule, and marking a mental health risk level; and S4, matching an intervention strategy, and dynamically updating the evaluation model and the symptom association rule. The multi-modal fusion strategy effectively covers an association path of a DLB patient from molecular pathology to clinical symptoms, further provides a high-reliability data basis for subsequent risk level marking, realizes extraction of pure alpha wave energy and correction of prefrontal lobe alpha wave asymmetry aiming at frequency spectrum overlapping interference of delta waves and alpha waves in electroencephalogram signals, and improves the accuracy of risk level marking. Emotion abnormal degree misjudgment caused by false increase of the FAA value is avoided.

The invention relates to the field of medical systems, in particular to a molecular pathology automatic diagnosis method and system and a storage medium, and the method comprises the steps: reading pathology data, and establishing a pathology database; reading case data; determining diagnosis data by using the case data and the pathological data; and outputting the diagnosis data and the case data corresponding to the diagnosis data to a user for confirmation. The method has the effect of improving the diagnosis efficiency.

The invention relates to the technical field of medical equipment, and discloses a slicing device for molecular pathology experiments. The cutting table is arranged on the base, and a cutter is arranged on the cutting table; the protective cover is arranged on the base, a first sliding groove is formed in the protective cover, a first sliding block is arranged in the first sliding groove in a sliding fit mode, a first bearing seat is arranged on the first sliding block, a main rod is rotationally matched with the first bearing seat, the first bearing seat does not limit the axial displacement of the main rod, a mounting head is arranged at one end of the main rod, and a wax block is mounted on the mounting head; the main driving mechanism is used for driving the first sliding block to slide back and forth in the first direction. The first driven mechanism is used for driving the main rod to rotate axially when the main rod moves along with the first sliding block; the second driven mechanism is used for driving the main rod to move in the second direction after the main rod reciprocates for one cycle in the first direction, low-loss and high-integrity cutting can be achieved, and the automatic and accurate feeding function is integrated.

This application relates to the field of molecular pathology diagnostic technology, specifically disclosing a bone marrow smear FISH detection kit and its application. The kit includes a rapid hybridization buffer, a stabilization pretreatment solution, room temperature hybridization probes, probe dilution buffer, low-salt washing buffer, high-salt washing buffer, counterstaining solution, positive control samples, and negative control samples. The room temperature hybridization probes include broken probe pairs targeting the BCR-ABL, PML-RARA fusion genes, and IgH gene rearrangements. The detection method of this kit includes sample pretreatment, probe denaturation, room temperature hybridization, washing and counterstaining, and signal interpretation. Through the synergistic effect of the rapid hybridization buffer and the short-chain low-Tm value probes, rapid hybridization at room temperature is achieved, eliminating the need for a dedicated isothermal hybridization instrument. The stabilization pretreatment solution can complete cell permeation and RNA removal in one step, resulting in a high degree of operational standardization.

The application relates to an experimental method for improving the accuracy of laboratory pollution monitoring and the quality control capability in molecular pathology detection. The method comprises the following steps: preparing a blank wax block without any tissue component while preparing a tissue paraffin-embedded sample to be detected; slicing the tissue paraffin-embedded sample and the blank wax block respectively; extracting nucleic acid of the tissue sample and nucleic acid of the blank wax block respectively; carrying out nucleic acid amplification detection by taking the nucleic acid of the tissue sample as a sample to be detected, taking the nucleic acid of the blank wax block as a negative whole-process control, and combining a positive control; judging the pollution and analyzing the quality control of the whole experimental process according to the detection result of the negative whole-process control of the blank wax block, so that the whole-process pollution risk of molecular pathology detection is continuously monitored. The application has the effects of improving the accuracy of laboratory pollution monitoring, the reliability of experimental results and the whole-process quality control capability of the laboratory.

The present invention discloses a lineage tracing mouse model, a construction method, and an application thereof, particularly an application in establishing a mouse model of acute drug-induced liver injury. The lineage tracing mouse model provided by the present invention is constructed from the offspring produced by mating Cyp2e1-DreERT2 mice with H11-tdTomato mice. It can specifically label CYP2E1-positive central venous zone (PC) hepatocytes. Therefore, in an acute drug-induced liver injury mouse model established by the lineage tracing mouse model, the cell fate or cell dynamic behavior of the remaining PC hepatocytes near the damaged area after acute drug-induced liver injury can be tracked. The spatiotemporal dynamic changes in the molecular pathology of PC hepatocytes and the molecular mechanism driving PC cell regeneration can be further analyzed.

The invention relates to the field of molecular pathology detection, and particularly discloses a hepatocellular carcinoma immunohistochemical diagnostic kit based on an aptamer technology, and the kit contains a DNA fragment with a nucleotide sequence SEQ No.1. The nucleotide sequence SEQNo.1 provided by the invention has the characteristics of high sensitivity, specificity, low cost, good stability and the like on hepatocellular carcinoma detection, provides a brand new method for pathological diagnosis of hepatocellular carcinoma, overcomes the defects of complex operation, long detection period and high cost of a traditional antibody detection method, and has a wide application prospect. By adopting the immunohistochemical pathological diagnosis kit disclosed by the invention, the diagnosis accuracy can be greatly improved, and the problem of missing detection caused by a high-cost and low-accuracy detection method is reduced.

The invention discloses a depigmentation method suitable for melanoma gene detection, and belongs to the technical field of molecular pathology, the depigmentation method comprises the following steps: pre-treating a melanoma sample, adding into a first part of treating fluid, soaking at 80 DEG C for 20 minutes, adding into a second part of treating fluid, soaking at 80 DEG C for 10 minutes, washing with water, and airing; a preparation method of the first part of treating fluid comprises the following steps: diluting H2O2 with a Tris-HCl buffer solution until the volume concentration of H2O2 is 1%, and then adding NaCl until the mass concentration of NaCl is 1% to obtain the first part of treating fluid; according to the depigmentation method, rapid and efficient depigmentation can be achieved for melanoma with different melanin contents, the DNA integrity and amplification efficiency of the melanoma treated through the depigmentation method are high, and the problem of depigmentation is not prone to occurring.

The molecular pathology experiment reagent titration device comprises a lower supporting plate, the projection of the lower supporting plate in the overlook direction is square, and height adjusting mechanisms are arranged at the four corners of the lower supporting plate; a frame body is arranged on the lower supporting plate, a sliding block is arranged on the frame body, two side wing parts of the sliding block are in sliding fit with a vertical plate of the frame body, a rope hanging body is arranged at the top in the frame body, a vertical cone is arranged at the lower part of the rope hanging body, and a positioning cone is fixedly arranged at the lower part in the frame body, so that the perpendicularity of the frame body is convenient to adjust; better use is facilitated; the spine portion of the lower portion of the vertical cone is aligned with the spine portion of the upper portion of the positioning cone, then the height adjusting mechanism is used for adjusting the state of the lower supporting plate, the lower supporting plate is parallel to the horizontal plane, and therefore the frame body is kept vertical.

The application discloses a depigmentation method suitable for melanoma gene detection and belongs to the technical field of molecular pathology. The depigmentation method comprises the following steps: after pretreatment of a melanoma sample, the first treatment solution is added, and the sample is soaked at 80 DEG C for 20 min; the second treatment solution is added, and the sample is soaked at 80 DEG C for 10 min; the sample is washed with water and dried; the preparation method of the first treatment solution comprises the following steps: H2O2 is diluted to 1% of the volume concentration of H2O2 by using Tris-HCl buffer solution, then NaCl is added to 1% of the mass concentration of NaCl, and the first treatment solution is obtained; the depigmentation method can realize rapid and efficient depigmentation for melanomas with different melanin contents, the DNA integrity and amplification efficiency of the melanoma treated by using the depigmentation method are high, and the problem of sample loss is not prone to occurring.

The invention discloses a multi-photon microscopic image automatic segmentation and regional quantitative feature extraction method based on a weak supervision pseudo label and application thereof. The method is based on lung cancer Hamp; and E, performing multi-photon imaging on the dyed paraffin section, and collecting SHG and TPEF multi-channel high-resolution images. A classification network with ResNet101 as a trunk is adopted, and pixel-level multi-class pseudo labels are generated. And inputting the pseudo tag as a supervision signal into a U-Net segmentation network to complete automatic segmentation of tumor, tumor-related interstitial substance, tumor necrosis and lymphocyte multi-category regions. Based on the segmentation result, multiple quantitative algorithms such as skeletonization, principal axis analysis, branch detection, area and density calculation, fractal and curvature analysis and the like are further integrated, and automatic extraction and standardized statistics of multi-dimensional morphology and spatial distribution characteristics of the target area are achieved. According to the method, the pixel-level manual labeling requirement is greatly reduced, the segmentation precision and the analysis automation level are improved, and the method is suitable for tumor microenvironment, matrix research, molecular pathology auxiliary diagnosis and other scenes.

This application belongs to the field of biomedicine, specifically relating to a method for evaluating the efficacy of Alzheimer's disease treatment based on multimodal data. It includes: constructing a personalized brain network model based on multimodal magnetic resonance imaging to locate hippocampal targets; implementing time-interventional electrical stimulation intervention; simultaneously collecting data from neuropsychological scales, electroencephalograms, event-related potentials, and blood biomarkers at four time points; constructing an efficacy evaluation system by integrating four-dimensional indicators: subjective cognition, objective electrophysiology, molecular pathology, and brain network regulation; and quantifying the intervention effect through principal component analysis and a linear mixed-effects model, and establishing a logistic regression classifier based on baseline features to predict individual response probabilities. This method achieves precise targeted intervention and multi-dimensional dynamic efficacy evaluation, improving the objectivity of the evaluation, the depth of mechanism analysis, and the ability for individualized clinical decision-making.

The invention belongs to the technical field of drug screening, and discloses a tumor therapeutic drug screening method and system.The tumor therapeutic drug screening method comprises the steps that an in-vivo tissue sample of a tumor patient is obtained, synchronous organoid culture is conducted, and an in-vitro organoid culture sample is obtained; performing general pathological evaluation and molecular pathological evaluation on the in-vivo tissue sample to obtain a plurality of basic evaluation results; performing an dry experiment on the in-vitro organoid culture sample by adopting a plurality of target treatment drugs, wherein the target treatment drugs are obtained by primarily screening the in-vivo tissue sample; performing general pathological evaluation, molecular pathological evaluation and in-vitro living cell pathological evaluation on the in-vitro organoid sample in an dry experiment process to obtain a plurality of experiment evaluation results; and analyzing the experimental evaluation result on the basis of the basic evaluation result to obtain a tumor therapeutic drug screening result. And the reliability of tumor therapeutic drug screening results can be improved.

The invention relates to the technical field of electrochemical sensing, in particular to an ultramicro electrode for monitoring chloride ions in real time and a preparation method and application of the ultramicro electrode. The ultramicro electrode comprises a hollow micron tube, an organic phase reference electrode and a water phase reference electrode / counter electrode; the tip of the hollow micron tube is filled with an organic phase, the organic phase reference electrode is inserted into the organic phase, the organic phase reference electrode and the water phase reference electrode / counter electrode are connected through a wire, and the organic phase comprises an organic solvent, organic gel powder and an ionic ligand. When the chloride ions are monitored, ion ligands in an organic phase and the chloride ions in a water phase to be detected are subjected to reversible specific binding at a liquid-liquid interface to accelerate an ion transfer reaction, so that the ultramicro electrode can monitor an ion transfer current, and real-time monitoring of the chloride ions is realized; the ultramicro electrode has good linear responsiveness, selectivity, reversibility and stability, and has wide application prospects in molecular pathology of nervous system diseases and related diagnosis and treatment aspects.

The present invention provides a deep learning-based microsatellite instability colorectal cancer mechanism information analysis system, which includes: a data set establishment module, a gene screening module, a training model establishment module and a learning model establishment module; the present invention uses a large number of colorectal cancer surgical specimens to establish a complete molecular pathology detection platform, clarifies the mutant genes in each case, and combines an artificial intelligence deep learning model, using surgical specimen tissue pathology digital slices as a training data set, grouping according to different mutant genes, and combining family medical history and clinical follow-up information to develop a deep learning-based computer-aided diagnosis system for the molecular mechanism of microsatellite instability colorectal cancer and the pathogenesis and prognosis prediction of Lynch syndrome patients; it can reduce the multi-step detection process in the Lynch syndrome screening process, save time, manpower and detection costs, reduce missed diagnoses, and can also provide the possibility of remote diagnosis by scanning pathology images.

This invention discloses a Raman spectroscopy-based detection technology for glioma IDH mutations, belonging to the field of tumor diagnosis. This invention prepares a core-shell structure based on gold nanoparticles and UiO-66, utilizing... N, N '-Di(2-aminoethyl)-1,3-propanediamine was used to modify the Raman signal of SERS-enhanced samples. Ratio analysis was then used to determine the presence of IDH mutations in gliomas, establishing a detection protocol for IDH mutations in glioma cells. This facilitates rapid intraoperative molecular pathology identification and provides effective guidance for surgical and subsequent treatment selection. The detection technology disclosed in this invention can obtain results within 2 minutes, greatly improving clinical diagnostic efficiency and enabling intraoperative molecular pathology diagnosis, which has significant clinical implications.

Human skeletal muscle stem cells were generated from facioscapulohumeral muscular dystrophy (FSHD) and healthy control iPSC using a transgene-free skeletal muscle differentiation protocol and production of stable iMyoblasts. Analyses revealed that FSHD and healthy control iMyoblasts are embryonic-like myogenic cells that undergo myotube differentiation ex vivo by growth factor depletion and are efficiently transplantable into the tibialis anterior (TA) muscles of NSG mice, where human muscle under-goes embryonic-to-adult myosin isoform switching. The DUX4 FSHD disease gene maintains its hypomethylated disease state inFSHD iPSC and iMyoblast, and its expression is upregulated during myotube differentiation and in muscle xenografts. Consequently, these iMyoblasts accurately exhibit the molecular pathology of human muscular dystrophies and are useful for the development of drug, gene editing and stem cell therapeutics.

The invention is applicable to the technical field of biological medicines, and provides application of ginsenoside CK in preparation of a medicine for preventing or treating Alzheimer's disease. Experiments prove that the ginsenoside CK can significantly improve the spatial learning and memory ability of AD model animals and relieve anxiety behaviors of the AD model animals; the deposition of brain tissue hippocampal Abeta (1-42) protein is effectively reduced, and the key molecular pathology of AD is relieved from the source; the level of key inflammatory factors in brain tissues and serum is strongly inhibited, and AD-related chronic neuroinflammation is controlled; according to the present invention, the CK has the specific protection effect on the hippocampal neuron structure, such that the CK can be adopted as the multi-target treatment agent so as to prevent and / or treat the Alzheimer's disease.

The application provides an auxiliary decision-making method and device for optimal wax blocks under molecular pathology special examination doctor's orders, and relates to the technical field of molecular pathology detection and medical artificial intelligence. The method comprises the following steps: in response to the order input operation of the user interface, obtaining the identity in the molecular pathology special examination doctor's order, and based on the identity, calling the preset information of a plurality of candidate wax blocks. For each candidate wax block, the missing data in the preset information is compensated and the wax block confidence is generated; based on the compensated data, the detection of multiple indexes is carried out, and the score of each index is generated. The compensated preset information of each candidate wax block is automatically compared, and the conflict information between each candidate wax block is generated; according to the conflict information, the wax block confidence is updated. Based on the score of each candidate wax block and the updated wax block confidence, the multiple candidate wax blocks are graded, and a detection strategy containing the main examination wax block recommendation is generated. The method is used to improve the existing wax block detection accuracy and detection efficiency.