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Kit for extracting DNA from biological tissue sample by paraffin imbedding method and extraction method

A technology of biological tissue and paraffin embedding, applied in the field of molecular biology, can solve the problems of low yield and deoxyribonucleic acid can not meet the requirements of molecular pathological detection, etc., and achieve the effect of high content

Inactive Publication Date: 2016-11-23
上海革亚医疗科技有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This traditional extraction method has a low yield, and the extracted deoxyribonucleic acid cannot meet the requirements of molecular pathology detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Embodiment 1 The present invention provides a kit for extracting DNA from biological tissue samples by paraffin embedding, including dewaxing solvent A, buffer B, protein digestion solution C, nucleic acid purification column D, and eluent E. Wax solvent A contains SDS, NP40, NaCL and buffer B contains EDTA, NaCL and SDS.

[0009] The present invention also provides a method for extracting DNA from a biological tissue sample using a kit, which specifically includes the following steps:

[0010] 1) Dewaxing: Take 3 paraffin sections with a thickness of 10-20 microns, add dewaxing solvent A, place in a water bath at 90°C for half an hour, let stand at room temperature for half an hour, centrifuge at 14000 rpm for 15 minutes, discard the supernatant Then add 70% ethanol, mix well, and centrifuge again at a centrifugation speed of 14,000 rpm for 15 minutes, discard the supernatant;

[0011] 2) Digestion:

[0012] Add 200 μL of buffer B and 10 μL of protein digestion solut...

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Abstract

The invention discloses a kit for extracting DNA from a biological tissue sample by a paraffin imbedding method. The kit comprises a deparaffinating solvent A, a buffer solution B, protein digestive fluid C, a nucleic acid purification column D and eluate E, wherein the deparaffinating solvent A contains SDS, NP40 and NaCL, and the buffer solution B contains EDTA, NaCL and SDS. According to the kit for extracting the DNA from the biological tissue sample by the paraffin imbedding method, disclosed by the invention, the quality of the extracted DNA fully meets the requirements of molecular pathology detection, and the extracted DNA is high in content.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a DNA extraction method, in particular to a kit for extracting biological tissue sample DNA by a paraffin embedding method and an extraction method thereof. Background technique [0002] The extraction of deoxyribonucleic acid from paraffin-embedded tissue is a routine work in clinical pathology. It must go through dewaxing, lysing tissue, digesting protein and ribonucleic acid, and finally purifying to obtain deoxyribonucleic acid. At present, the commonly used paraffin sample extraction kits use xylene dewaxing, protease digestion of protein, ribonuclease digestion of ribonucleic acid, and then use nucleic acid purification columns to purify to obtain deoxyribonucleic acid. This traditional extraction method has a low yield, and the extracted deoxyribonucleic acid cannot meet the requirements of molecular pathology detection. Contents of the invention [0003] The pu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
CPCC12N15/1003
Inventor 戚行泽胡洪良
Owner 上海革亚医疗科技有限公司
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