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Formaldehyde-free fixative for tissue sample
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A technology of tissue specimens and fixatives, applied in the field of pathology experiments, can solve the problems of poor preservation of nucleic acid molecules, shrinkage hardening, etc., and achieve the effects of avoiding poisoning, eliminating covering, and inhibiting shrinkage hardening
Inactive Publication Date: 2013-04-03
HARBIN GREEN SPECIMEN TECH DEV
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However, shrinkage and hardening will occur in the hinge reaction, and the antigen molecular structure, enzymes, and nucleic acid molecules of tissue cells are not well preserved.
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[0049] The above components are miscible at room temperature.
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Abstract
The invention provides a formaldehyde-free fixative for a tissue sample, which comprises the following ingredients in parts by volume: 710-790 parts of ethanol, 20-40 parts of glacial acetic acid, 10-30 parts of acetone, 40-60 parts of polyhydric alcohol, 40-60 parts of trichloroacetic acid (TDA), 40-60 parts of surface active agent and 40-60 parts of solubilizing agent. The formaldehyde-free fixative is a nontoxic and innocuous chemical reagent; after being proved by cellular morphological experiments, immunohistochemical experiments, molecular pathological experiments and nucleic acid extraction experiments, the formaldehyde-free fixative has relatively comprehensive fixing effect, can replace the formaldehyde and be used in related pathological experiments, thereby fixing the tissue sample.
Description
technical field [0001] The invention relates to in vitro diagnostic reagents, in particular to an environment-friendly formaldehyde-free tissue specimen fixative and its application, belonging to the technical field of pathological experiments. Background technique [0002] The birth of histopathology has a history of more than 150 years. In all hospitals, the pathological specimens of surgical resection and various biopsy tissues are all histopathologically diagnosed by the hospital pathology department on the surgical tissue samples. [0003] Conventional paraffin-embedded HE staining is the most basic technique currently used to process tissue samples, it is the basis of pathological diagnostic methodology, and it is also the reference standard for evaluating the advantages and disadvantages of many new methods and technologies. The traditional method is to place the tissue sample in the tank of the treatment solution, pour or transfer in different treatment solutions in ...
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