Biological leaching restoring method for heavy metal polluted soil

A technology for polluted soil and biological leaching, applied in the field of environmental engineering, can solve problems such as secondary pollution, and achieve the effects of improving fertility, remediation efficiency, and improving soil physical and chemical properties.

Active Publication Date: 2012-07-04
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The remediation ideas of heavy metal-contaminated soil can be summarized into two types: one is that heavy metals still exist in the soil, and the toxicity of heavy metals can be reduced by adding chemical reagents or microorganisms to the soil, so as to achieve the purpose of treatment. A potential weakness of this method Because heavy metals still exist in the soil, if the soil environment changes, it may cause secondary pollution

Method used

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  • Biological leaching restoring method for heavy metal polluted soil
  • Biological leaching restoring method for heavy metal polluted soil
  • Biological leaching restoring method for heavy metal polluted soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Bioleaching restoration of compound polluted soil in a smelting waste dump of a smelter in Zhuzhou, Hunan

[0030] Pick the Penicillium chrysogenum F1 bacterium colony respectively, in Cha's solid medium (sucrose 30g, K 2 HPO 4 1g, NaNO 3 3g, MgSO 4 0.5g, KCl 0.5g, FeSO 4 0.01g, 20g agar, add H 2 0 to 1L; use 0.1mol L-1 HCl to adjust the pH value of the medium to 6.7) and cultivate to obtain the strain spores for inoculation. After 7 days, use a sterile pipette to draw sterile water to rinse the surface of Penicillium chrysogenum F1 colonies to prepare Penicillium chrysogenum F1 10 7 order of magnitude spore fluid.

[0031] The collected soil under the smelting waste pile in a smelter plant in Zhuzhou, Hunan was air-dried, ground, passed through a 2mm aperture sieve, and sterilized in a 150°C sterilizing pot for 30 minutes. Weigh glucose 90g, K 2 HPO 4 1g, NaNO 3 3g, MgSO 4 0.5g, KCl 0.5g, FeSO 4 0.01g dissolved in 1L H 2 O, use 0.1mol L ...

Embodiment 2

[0033] Example 2 Comparison of effects of remediating heavy metal complex pollution using different leaching methods Penicillium Chrysogenum F1

[0034]Take 49mL of modified Cha's liquid medium and put it into a 250mL Erlenmeyer flask for sterilization, and inoculate about 10 7 Add 2.5g of sterilized soil to 1mL of spore liquid. Place in a constant temperature shaking incubator (25°C, 120r / min) and shake for 15 days. The experiment was divided into 3 groups. The first group was a one-step method, that is, soil was added while inoculating the spore liquid. The second group was a two-step method, that is, the bacteria were cultured for 7 days before adding soil. The third group was the control, and only soil was added. , not inoculated with spore fluid. Each experiment was repeated three times. Take out and filter after 15 days, and the filtrate is used to measure the concentration of heavy metals, which is the concentration after leaching, and the measurement of the concentr...

Embodiment 3

[0035] Example 3 Penicillium Chrysogenum F1 remediation of unsterilized heavy metal compound polluted soil

[0036] Take 49mL of modified Cha's liquid medium and put it into a 250mL Erlenmeyer flask for sterilization, and inoculate about 10 7 Add 2.5g of unsterilized soil to 1mL of spore liquid of order of magnitude. Place in a constant temperature shaking incubator (25°C, 120r / min) and shake for 15 days. The experiment was divided into two groups. The first group cultured the bacteria for 7 days before adding non-sterilized soil. The second group was the control group. The bacteria were cultured for 7 days before adding sterilized soil. Each experiment was repeated three times. Take out and filter after 15 days, and the filtrate is used to measure the concentration of heavy metals, which is the concentration after leaching, and the measurement of the concentration of heavy metals is measured by ICP-AES. After testing, the removal rate of each heavy metal in the first group...

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Abstract

The invention discloses a biological leaching restoring method for heavy metal polluted soil. After Penicillium chrysogenum F1 spore liquid is cultured in an improved Czapek liquid medium, leaching fungus liquid is obtained, and then the heavy metal polluted soil is leached, dried and crushed. By restoring the heavy metal (Pb, Zn, Cd and Cu) compound polluted soil according to the biological leaching restoring method provided by the invention, the removing rate of Pb in the soil is 14%, the removing rate of Cd is 63%, the removing rate of Zn is 54% and the removing rate of Cu is 56%. The biological leaching restoring method for the heavy metal polluted soil provided by the invention has the advantages of high speed in taking effect, high efficiency in removing heavy metal, absence of secondary pollution, and the like.

Description

technical field [0001] The invention belongs to the field of environmental engineering, and relates to a bioleaching restoration method for heavy metal polluted soil, in particular to a bioleaching restoration method for treating heavy metal contaminated soil by using bacteria strains, in particular to a bioleaching restoration method for heavy metal contaminated soil in a lead-zinc smelting waste dump. Background technique [0002] With the intensification of industrial and urban pollution and the increase in the types and quantities of agricultural chemicals, soil heavy metal pollution is becoming more and more serious. At present, the world's average annual emission of Hg is about 15,000 tons, Cu is 3.4 million tons, Pb is 5 million tons, Mn is 15 million tons, and Ni is 1 million tons. According to the national survey of sewage irrigation areas conducted by the Ministry of Agriculture of my country, in about 1.4 million hm 2 Among the sewage-irrigated districts in China...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
Inventor 柴立元杨志辉邓新辉杨卫春唐崇俭
Owner CENT SOUTH UNIV
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