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Method for improving salt resistance of turf by adopting reinforced garbage compost microbial agent

A technology of microbial agent and composite microbial agent, which is applied in the field of strengthening municipal solid waste composting microbial agent and improving the salt resistance of carpet and turf

Inactive Publication Date: 2015-07-22
TIANJIN NORMAL UNIVERSITY
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  • Abstract
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  • Method for improving salt resistance of turf by adopting reinforced garbage compost microbial agent
  • Method for improving salt resistance of turf by adopting reinforced garbage compost microbial agent

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Embodiment 1

[0091] Preparation method of strengthening salt-tolerant microbial bacterial agent

[0092]（1） Prepare the concentration of salt (w / w): prepare the concentration of NaCl respectively: 0.5%, 1%, 1.5%, 2%, 2.5% and 3% for later use;

[0093] (2) During the domestication process, depending on the OD 600 Gradually increase the salt concentration, the concentration of NaCl is increased to 30g / L with a gradient of 5g / L, every time the concentration of NaCl is increased, after the growth of the bacteria is stable, the concentration of NaCl will continue to be increased, and the salt-tolerant mixture will be acclimated step by step. Microflora; Bacillus subtilis, Bacillus lysinus and Penicillium chrysogenum;

[0094] (3) Enhanced culture of salt-tolerant strains: expand the screened enhanced salt-tolerant microorganisms on the corresponding liquid medium, culture Bacillus subtilis and Bacillus lysine at 30°C, 180r / min for 1 day, and produce yellow and green Mold was cultured at 28°C...

Embodiment 2

[0097] Method of Improving Salt Resistance of Turf by Strengthening Garbage Composting Microbial Inoculum :

[0098] (1) Experimental materials: Lawn grasses were selected to select tall fescue with full grains and uniformity ( Festuca arundinacea L.) Seeds are the test materials;

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Abstract

The invention discloses a method for improving the salt resistance of turf by adopting a reinforced garbage compost microbial agent. The reinforced salt resistant microbial agent comprises the following components: reinforced bacillus subtilis, reinforced lysine bacillus and reinforced penicillium chrysogenum, with the weight ratio of 1: 1: 1. The method disclosed by the invention adopts the following steps: gradually increasing the concentration of NaCl to reinforce microorganisms in urban garbage compost, and finally performing the screening so as to obtain the prepared reinforced salt resistant microbial agent. The obtained reinforced salt resistant microorganisms of the reinforced bacillus subtilis, the reinforced lysine bacillus and the reinforced penicillium chrysogenum are compounded into composite microbial agents in different concentrations, the composite microbial agents are inoculated to a planting system of the turf, so that the optimal concentration of the composite reinforced salt resistant microbial agent is screened out, the salt resistance of the turf is improved, and the technical support is provided for planting the carpet turf on the substrate of saline-alkali soil.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to a method for strengthening municipal solid waste composting microbial bacterial agents and improving the salt resistance of carpet turf. Background technique [0002] Microbial agents, especially compound microbial agents composed of a variety of microorganisms, are biotechnology developed in recent years with broad application prospects. Microbial agents can not only repair oil-contaminated soil and degrade organic pollutants in water bodies, but also prevent and control pests and diseases, promote crop production and increase production and income. Some microbial agents can improve the stress resistance of plants. Since the 1970s, European and American Japan and other countries or regions have successfully developed some composite bacterial agents, many of which have begun large-scale production and formed serialized products. Among them, EM (Effective Microorga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A01G1/00A01G7/06C05F17/00C12R1/125C12R1/82C12R1/07
CPCA01G7/06A01G22/00C05F11/08C12N1/14C12N1/20Y02W30/40
Inventor 多立安赵树兰高星星
Owner TIANJIN NORMAL UNIVERSITY
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