The invention relates to promoters of the genes 
glutamate dehydrogenase, beta-acetylhexosaminidase and gamma-
actin and their use in systems of expression, 
secretion and anti-sense of filamentary fungi. The invention also relates to the use of the promoters of the genes which code: (I) 
glutamate dehydrogenase NADP depending (EC.1.4.1.4) of 
Penicillium chrysogenum, (II) gamma-N-actylhexosaminidase (EC.3.2.1.52) of 
Penicillium chrysogenum and (III) gamma-
actin of 
Penicillium chrysogenum and Acrimonium chrysogenum, which can be used for the construction of potent vectors of expression and 
secretion useful both for P. chrysogenum and for A. chrysogenum and related species. These promoters can also be used for blocking the genic expression through anti-sense construction. Under the control of the above mentioned promoters, it is possible to conduct the expression of other genes in filamentary fungi, thereby increasing the production of 
antibiotics and / or proteins inherent to the same.