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Preparation capable of inducing cucumber to resist meloidogyne and preparation method for preparation

A technology against root-knot nematodes and cucumbers, applied in the directions of nematicides, botanical equipment and methods, microorganism-based methods, etc., can solve the problem of low induction intensity and so on

Inactive Publication Date: 2013-01-16
HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with chemical insecticides, there is still a large gap in the control effect of existing chitosan oligosaccharides on plant parasitic nematodes, and there is a problem of low induction strength, which limits the use of chitosan oligosaccharides in the control of cucumber root-knot nematodes. Applications

Method used

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Embodiment Construction

[0012] The present invention will be further described below by embodiment.

[0013] Its preparation method is as follows:

[0014] 1. Preparation of chitosanase fermentation broth: prepare liquid medium with the following components and percentage by weight: chitosan: 15g, yeast extract: 0.5g, NH 4 Cl: 1.5g, Glucose: 1.0g, KH 2 PO 4 : 1.0g, MgSO 4 ·7H 2 O : 0.5g, MnSO 4 : 0.5g, water: 980g, natural pH, sterilized at 121°C for 30 minutes, inoculated with Paecilomyces fungi producing chitosanase ( Paecilomyces sp.), cultivated at 28°C for 2 days, filtered the fermentation broth with a plate frame, and the filtrate was chitosanase fermentation broth;

[0015] 2. Preparation of chitosan oligosaccharide solution: use 1.5% glacial acetic acid solution as solvent, prepare 100L of chitosan solution with a concentration of 4%, add 2L of chitosan enzyme fermentation broth under stirring, stir and react at 50°C for 3h, plate Frame filtration gets chitosan oligosaccharide solut...

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Abstract

A preparation capable of inducing cucumber to resist meloidogyne is characterized in that the preparation is prepared from a chitosan oligosaccharide solution and sodium salicylate, wherein the weight ratio of the chitosan oligosaccharide solution to the sodium salicylate is 10-100:1-10. The preparation method comprises the following steps of: cultivating fungus capable of producing chitosanase through fermentation by taking chitosan, yeast extract, NH4Cl, glucose, KH2PO4, MgSO4.7H2O, MnSO4 and water as a culture medium; preparing fermentation liquor containing chitosanase; and performing enzymolysis on the chitosan through the fermentation liquor to obtain the chitosan oligosaccharide solution, and mixing the chitosan oligosaccharide solution and the sodium salicylate to prepare the preparation. The preparation provided by the invention can be used for preventing and controlling the cucumber meloidogyne, has a control effect of 50 to 70 percent, can simultaneously promote growth of cucumber root and has a remarkable effect on ecological soil remediation.

Description

technical field [0001] The invention relates to a preparation for inducing cucumber resistance to root-knot nematodes and a preparation method thereof. Background technique [0002] Cucumber root-knot nematode disease caused by root-knot nematode (Meoidogyne spp.) is a worldwide disease. When the disease occurs, it generally causes economic losses of 10%-15% per year, and economic losses can reach 30%-40% when the disease is severe. %, even extinct. In recent years, the rapid development of cucumber in greenhouses and the widespread phenomenon of repeated cropping have led to the accumulation of root-knot nematodes in the soil, and the damage has increased year by year. The control of cucumber root-knot nematode mainly relies on chemical insecticides, but they can only control the number of nematodes in a short period of time. At the same time, there are still disadvantages such as environmental pollution, excessive residues of agricultural products, and drug resistance. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01N37/40A01P5/00A01P21/00C12P19/26C12R1/79
Inventor 史凤玉朱英波
Owner HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
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