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SSR (simple sequence repeat) markers, linked with LRPW (loss rate of panicle weight) related Aphelenchoides besseyi Christie resistant QTL (quantitative trait loci), on chromosome 5 and application thereof

A technology for resistance to A. rice nematode, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of limiting the effective use of resources for resistance to A. rice, and improve breeding efficiency , speed up the breeding process, simplify the effect of selection methods

Inactive Publication Date: 2014-08-13
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the quantitative trait locus (Quantitative Trait Locus, QTL) or gene of the resistance of rice varieties to A. sativa. The research on the resistance to A. sativa is only at the level of variety resistance identification This greatly limits the effective utilization of rice A. spp. resistance resources in resistance breeding

Method used

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  • SSR (simple sequence repeat) markers, linked with LRPW (loss rate of panicle weight) related Aphelenchoides besseyi Christie resistant QTL (quantitative trait loci), on chromosome 5 and application thereof
  • SSR (simple sequence repeat) markers, linked with LRPW (loss rate of panicle weight) related Aphelenchoides besseyi Christie resistant QTL (quantitative trait loci), on chromosome 5 and application thereof
  • SSR (simple sequence repeat) markers, linked with LRPW (loss rate of panicle weight) related Aphelenchoides besseyi Christie resistant QTL (quantitative trait loci), on chromosome 5 and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Example 1, Acquisition of Molecular Markers Linked to the Resistance QTL of D.

[0020] 1. Plant material

[0021] In 2008, Huaidao 5 and Tetep were planted in the field of Jiangsu Academy of Agricultural Sciences and crossed to obtain the hybrid F 1 , next year F 1 F 2 Seeds, propagated F in Hainan in 2009 2 group, as a mapping group. f 2 Individual plant number, parent and hybrid F at tillering stage 1 and F 2 Some leaves of each individual plant in the population were stored in a -70°C refrigerator for SSR analysis, F 2 Harvest the individual plants of the population for phenotypic identification.

[0022] 2. Cultivation and isolation of nematodes

[0023] Inoculate Botrytis cinerea (Botrytis cinerea) bacterial block on Potato Dextrose Agar (PDA) medium and cultivate at 25°C. After Botrytis cinerea overgrows the medium, use 3% hydrogen peroxide to sterilize the surface of rice dry acerbone nematode for 10min , after washing with sterilized ultrapure water f...

Embodiment 2

[0060] Example 2, the application of molecular markers on chromosome 5 linked to the resistance QTL of rice stem nematode resistance to rice 5 and Tetep in the offspring of the cross combination with Huaidao 5 and Tetep as parents

[0061] The SSR marker obtained on chromosome 5 linked to the rice stem nematode resistance QTL, the F 2∶3 Part of the individual plants of the family were predicted for the resistance of rice stem nematode, and the DNA of each individual plant was extracted, and then PCR amplification analysis was performed with the primers of SSR markers RM163, RM18620, RM440 and RM161, and the band type analysis was used to determine whether there were corresponding The presence of the marker indicates that the line has reached the level of resistance to A. sativa, and the absence of the marker indicates that it is susceptible to the disease. Subsequently, the actual resistance of the tested lines to rice A. spp. was determined by using the artificial inoculation...

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Abstract

The invention discloses molecular markers linked with Aphelenchoides besseyi Christie resistant QTL (quantitative trait loci). The essence of the molecular markers is that according to the linkage and segregation rules, the population of the hybrid generation F2 of the Aphelenchoides besseyi Christie resistant variety Tetep and the susceptible variety Huaidao 5 is taken as the mapping population, and the SSR (simple sequence repeat) markers RM163, RM18620, RM440 and RM161, linked with two LRGW (loss rate of panicle weight) related Aphelenchoides besseyi Christie resistant QTL, on the chromosome 5 are obtained through analysis of linkage between the identification results of Aphelenchoides besseyi Christie resistance and the SSR marker data. Whether a plant has Aphelenchoides besseyi Christie resistance can be judged by detecting the SSR marker banding pattern of the single rice plant of the hybrid combination generation of Tetep and derived varieties (lines) thereof and the Aphelenchoides besseyi Christie susceptible variety. After being applied to assistant selection breeding of the Tetep and derived varieties (lines) thereof / susceptible variety-Aphelenchoides besseyi Christie resistant variety, the SSR markers can overcome the defects of poor stability and repeatability of Aphelenchoides besseyi Christie resistance identification, time and labor waste and the like in conventional breeding and can simplify the selection method and improve the breeding efficiency so as to accelerate the breeding process of the Aphelenchoides besseyi Christie resistant variety.

Description

Technical field: [0001] The invention relates to molecular markers RM163, RM18620, RM440 and RM161 linked to two LRPW-related QTLs (qLRPW5a, qLRPWSb) for resistance to rice stem nematode resistance on rice chromosome 5, which can be applied to molecular marker-assisted breeding and belong to rice disease-resistant breeding and molecular biology. Background technique: [0002] Rice dry point nematode is a kind of rice ectoparasitic pathogen, and its typical symptom is that leaf tip twists into dry point, once this disease takes place, it will cause serious loss of grain yield (Journal of the faculty of Agriculture, Kyushu, University, 1950, 9 (3): 309-333; Nematologica, 1975, 21(3): 351-357; Journal of Agricultural Technology, 2011, 337-344). The traditional control methods of A. rice nematode are costly and cause serious environmental problems (Journal of Agricultural Science and Technology, 2011, 14(1): 195-203). Utilizing the resistance of rice varieties is the most econ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 周彤杜琳琳周益军高存义兰莹孙枫
Owner JIANGSU ACAD OF AGRI SCI
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