Thioredoxin-like genes, primers and applications for controlling rice stem nematode

A technology of thioredoxin-like nematode of rice, applied in the direction of chemical, application and nematicide for biological control, can solve the problem of less research on plant parasitic nematode Trx, and achieve the effect of avoiding environmental pollution

Inactive Publication Date: 2020-03-17
NORTHEAST FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
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  • Thioredoxin-like genes, primers and applications for controlling rice stem nematode
  • Thioredoxin-like genes, primers and applications for controlling rice stem nematode
  • Thioredoxin-like genes, primers and applications for controlling rice stem nematode

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Embodiment 1

[0025] Acquisition and functional verification of thioredoxin-like genes for the control of Oryza sativa.

[0026] (1) RNA extraction and cDNA synthesis:

[0027] Nematodes were washed with DEPC-treated water (female: male: larva = 4:2:1), centrifuged to remove the supernatant, and ground under liquid nitrogen treatment. Take the ground powder, apply the TRIzol method (Invitrogen, cat. No. 15596-026) to extract the total RNA of A. sativa, and dissolve it in DEPC-treated water. Apply AMV reverse transcription system (Promega, cat.No.A3500), with Oligo(dT) 18 The first-strand cDNA was reverse-transcribed with primers, and the second-strand cDNA was synthesized by random primer method. The reaction process was carried out according to the test manual.

[0028] (2) Cloning of the complete reading frame of the thioredoxin-like gene of the rice stem nematode

[0029] According to the results of transcriptome sequencing, a primer set of thioredoxin-like gene was synthesized from ...

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Abstract

Thioredoxin-like gene for preventing and treating Aphelenchoides besseyi has a sequence shown as in sequence table SEQ No. 1. The sequence of the thioredoxin-like gene is shown as in the sequence table SEQ No. 1. A primer for constructing the thioredoxin-like gene for Aphelenchoides besseyi is provided, upstream primer Ab-Trxl-F is shown as in sequence table SEQ No. 2, and downstream primer Ab-Trxl-R is shown as in sequence table SEQ No. 3. The thioredoxin-like gene is applied to prevent and treat Aphelenchoides besseyi. The thioredoxin-like gene experiences up-regulated expression after Aphelenchoides besseyi is stressed by high osmotic pressure, it is indicated that the thioredoxin-like gene plays a role in the process of Aphelenchoides besseyi resisting the stress of high osmotic pressure. Studies on the thioredoxin-like gene show that the thioredoxin-like gene has important biological significance and potential applicable value in preventing and treating Aphelenchoides besseyi.

Description

technical field [0001] The invention relates to a thioredoxin-like gene, a primer and an application thereof for preventing and treating the rice stem nematode. Background technique [0002] Rice (Oryza sativa L.) is one of the main food crops in the world and is the staple food of one-third of the human beings in the world. Rice Aphelenchoides besseyi Christie caused by Aphelenchoides besseyi Christie reduces rice production by 10% to 70% every year worldwide, causing huge economic losses. At present, the prevention and control measures for rice Apical nematode mainly include strengthening quarantine and soaking seeds with warm soup or chemicals before sowing, but the germination potential of rice often tends to decrease after soaking. The study found that in the process of rice cultivation, fertilization and pesticide application have an important impact on the osmotic pressure of the soil environment. Most organisms cannot survive under high osmotic pressure, but rice s...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12N15/12C12N15/11C12Q1/6895A01N37/46A01P5/00
CPCA01N37/46C07K14/4354C12Q1/6888C12Q2600/124C12Q2600/158
Inventor 王峰陈俏丽李丹蕾零雅茗
Owner NORTHEAST FORESTRY UNIVERSITY
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