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Primer group, system, kit and method for detecting aphelenchoides besseyi

A rice stem tip nematode and primer set technology, applied in the field of pathogen detection, can solve the problems of inability to realize early detection and 100% determination of negative and positive samples, so as to prevent rice stem tip disease, reduce synthesis cost, and reduce detection cost Effect

Active Publication Date: 2021-10-29
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, fluorescent quantitative PCR is a more effective method for detecting A. sativa. However, for samples with extremely low nucleic acid content, that is, with a Ct value of 30-40, it is impossible to be 100% sure that the sample is negative or positive.
It is impossible to realize early detection of rice seeds with very little amount of rice Apical nematode

Method used

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  • Primer group, system, kit and method for detecting aphelenchoides besseyi
  • Primer group, system, kit and method for detecting aphelenchoides besseyi
  • Primer group, system, kit and method for detecting aphelenchoides besseyi

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Embodiment 1

[0032] On the one hand, the embodiment of the present invention provides a primer set for detecting A. sativa. The primer set includes: a fifth crRNA, and the sequence of the fifth crRNA is shown as SEQ ID NO: 5 in the sequence listing.

[0033] Specifically, the primer set also includes: a first upstream primer and a first downstream primer, the sequence of the first upstream primer is as shown in SEQ ID NO: 9 in the sequence listing, and the sequence of the first downstream primer is as shown in SEQ ID NO in the sequence listing: 10 shown.

[0034] Specifically, the primer set further includes: a reporter group, and the sequence of the reporter group is shown in SEQ ID NO: 11 in the sequence listing.

[0035] On the other hand, an embodiment of the present invention provides a kit for detecting A. sativa. The kit includes the above-mentioned primer set.

[0036] In yet another aspect, the embodiment of the present invention provides a method for detecting A. sativa. The met...

Embodiment 2

[0054] The embodiment of the present invention provides a primer set for detecting the rice stem acerola. The primer set includes: the first crRNA, the second crRNA, the third crRNA, the fourth crRNA, the fifth crRNA, the sixth crRNA, the seventh crRNA and the first crRNA. Eight crRNAs, the sequence of the first crRNA is shown in SEQ ID NO: 1 in the sequence listing, the sequence of the second crRNA is shown in SEQ ID NO: 2 in the sequence listing, and the sequence of the third crRNA is shown in SEQ ID NO in the sequence listing: 3, the sequence of the fourth crRNA is shown in SEQ ID NO: 4 in the sequence listing, the sequence of the fifth crRNA is shown in SEQ ID NO: 5 in the sequence listing, and the sequence of the sixth crRNA is shown in SEQ ID NO in the sequence listing: As shown in 6, the sequence of the seventh crRNA is shown in SEQ ID NO: 7 in the sequence listing, and the sequence of the eighth crRNA is shown in SEQ ID NO: 8 in the sequence listing.

[0055] Specifica...

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Abstract

The invention provides a primer group, a system, a kit and a method for detecting aphelenchoides besseyi. The primer group comprises at least one of a first crRNA, a second crRNA, a third crRNA, a fourth crRNA, a fifth crRNA, a sixth crRNA, a seventh crRNA and an eighth crRNA. The embodiment of the invention provides the primer group, the system, the kit and the method for detecting the aphelenchoides besseyi, and the primer group can accurately and quickly detect the aphelenchoides besseyi and has high sensitivity and specificity. According to the method, a sample with the minimum concentration of 1 copy / mu L can be efficiently detected, and the fluorescence value can be increased by increasing the reaction time, so that the accuracy of the detection result is improved, and the detection of the amount of the aphelenchoides besseyi at the early stage can be realized, thereby preventing the aphelenchoides besseyi disease.

Description

technical field [0001] The invention relates to the field of pathogen detection, and more specifically, to a primer set, a system, a kit and a method for detecting the rice stem nematode. Background technique [0002] Rice stem-point nematode (Aphelenchoides besseyi Christie, 1942) belongs to the genus Aphelenchoides. Rice stem-point nematode is a slender worm-shaped, translucent, about 620-880 μm in length. The rice stem-point nematode mainly parasitizes the above-ground parts of plants, which will harm the growth and development of plants. Rice is the most important host of rice dry point nematode. When the disease occurs, the rice will appear "dry point" disease or "white point" disease, and reduce the yield of rice. In severe cases, the rice yield will be reduced by more than 50%. China is the largest rice producing country in the world, and the rice stem apical nematode is widely distributed in the mainland of China, and the damage of the disease has become more and mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6844C12N15/11
CPCC12Q1/6888C12Q1/6844C12Q2521/507C12Q2522/101C12Q2531/119C12Q2563/107Y02A50/30
Inventor 张安鹏曹黎明储黄伟程灿牛付安周继华孙滨
Owner SHANGHAI ACAD OF AGRI SCI
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