ATP synthetase gene for prevention and control of aphelenchoides besseyi christie, and primers and application thereof

A technology of rice nematode and synthase, which is applied in the direction of chemical, application, hydrolase for biological control, etc., can solve the problem that ATP synthase gene regulates the function of nematode anti-hypoxic stress and other problems.

Inactive Publication Date: 2017-04-26
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are relatively many studies on plant ATP synthase at home and abroad, but there are few studies on the function of ATP synthase gene regulating nematode resistance to hypoxic stress

Method used

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  • ATP synthetase gene for prevention and control of aphelenchoides besseyi christie, and primers and application thereof
  • ATP synthetase gene for prevention and control of aphelenchoides besseyi christie, and primers and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Obtaining and functional verification of the ATP synthase gene for the control of A.

[0025] (1) RNA extraction and cDNA synthesis:

[0026] The DEPC-treated water was used to wash the rice dry acerolae (female: male: larva = 4:2:1), centrifuged to remove water, and ground under liquid nitrogen. Take the ground powder and use TRIzol method (Invitrogen, cat. No. 15596-026) to extract total RNA. After DEPC treated water to dissolve RNA, apply AMV reverse transcription system (Promega, cat.No.A3500) to Oligo(dT) 18 As primers, first-strand cDNA is synthesized. According to the test manual, the second-strand cDNA was synthesized using the random primer method.

[0027] (2) Cloning of the Complete Reading Frame of the ATP Synthase Gene of Oryza sativa

[0028] According to the results of transcriptome sequencing, the primer set of ATP synthase gene of A.

[0029] Ab-ATPs-F:5`-TGT GAT GAT GCC GTC GAT TC-3`,

[0030] Ab-ATPs-R: 5`-CCT TAG CGT TCT TGG CTG TT-3`.

[0031...

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Abstract

The invention relates to an ATP synthetase gene for prevention and control of aphelenchoides besseyi christie, and primers and an application thereof. The gene has the sequence shown in a sequence table SEQ No.1. A primer group for construction of the aphelenchoides besseyi christie ATP synthase gene comprises an upstream primer Ab-ATPs-F shown in the sequence table SEQ No.2 and a downstream primer Ab-ATPs-R shown in the sequence table SEQ No.3. The application of the gene in prevention and control of the aphelenchoides besseyi christie is provided. The ATP synthetase gene has the expression up-regulated after the aphelenchoides besseyi christie is subjected to hypoxia stress, so the gene is indicated to play a role in the hypoxia stress resisting process of the aphelenchoides besseyi christie. Through the study of the gene, the gene has important biological significance and potential application value in the prevention and control of the aphelenchoides besseyi christie.

Description

technical field [0001] The invention relates to an ATP synthetase gene, a primer and application thereof for preventing and treating the rice stem nematode. Background technique [0002] Rice (Oryza sativa L.) is one of the most important food crops in my country and even in the world. Rice is infested by Aphelenchoides besseyi Christie, which causes huge economic losses. The world's annual output is reduced by 10-20%, and in severe cases, the output is reduced by more than 30%. At present, the prevention and control measures for rice A. achinensis are mainly to strengthen quarantine and soak seeds before sowing, but the control effect of soaking seeds is limited, and it affects the germination potential of rice. The rice stem nematode overwinters as adults and larvae hidden between the chaff and rice grains of the grain. When the seeds are soaked to accelerate germination, the nematodes in the seeds start to move. After sowing diseased seeds, most of the nematodes are fre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/55C12N15/11C12Q1/68A01N61/00A01P5/00
CPCA01N61/00C12N9/14C12N15/11C12N2310/10C12Q1/6876C12Q2600/158C12Y306/01003
Inventor 李丹蕾陈俏丽王峰零雅茗
Owner NORTHEAST FORESTRY UNIVERSITY
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